Springer Lab Manuals

Differential-Display Reverse Transcription-PCR (DDRT-PCR)

Authors: Colonna-Romano, Sergio, Leone, Antonella, Maresca, Bruno

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About this book

Identification of differentially expressed genes is one of the major challenges in molecular biology. Several techniques allow the cloning of such sequences. However, methods such as RNA subtraction or differential hybridization are time-consuming and require large amounts of mRNA.
Recently, a new approach has successfully been developed: Differential-Display Reverse Transcription-PCR (DDRT-PCR). This technique has been proven to be highly effective in identifying sequences that are differentially expressed in various cell types. The most striking advantage is, however, that only nanograms of total RNA are sufficient. Thus every mRNA species expressed in the cell system can be investigated, even those present at very low levels.

Table of contents (11 chapters)

Table of contents (11 chapters)

Buy this book

eBook 107,09 €
price for Spain (gross)
  • ISBN 978-3-642-80454-0
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Softcover 135,19 €
price for Spain (gross)
  • ISBN 978-3-540-63297-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
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Bibliographic Information

Bibliographic Information
Book Title
Differential-Display Reverse Transcription-PCR (DDRT-PCR)
Authors
Series Title
Springer Lab Manuals
Copyright
1998
Publisher
Springer-Verlag Berlin Heidelberg
Copyright Holder
Springer-Verlag Berlin Heidelberg
eBook ISBN
978-3-642-80454-0
DOI
10.1007/978-3-642-80454-0
Softcover ISBN
978-3-540-63297-9
Edition Number
1
Number of Pages
X, 124
Number of Illustrations
74 b/w illustrations
Topics