Editors:
- Detailed step-by-step protocols, including troubleshooting sections and many hints and comments from the authors
- The technology described is ideally suited to cope with the flood of data derived from the Human Genome Project
- The protocols are well established and are provided by authors with plenty of experience with this technology
Part of the book series: Springer Lab Manuals (SLM)
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Table of contents (11 chapters)
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Front Matter
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Back Matter
About this book
The technique of oligopeptide synthesis on a cellulose membrane and the subsequent binding assays allow the investigation of protein interactions. A particular advantage of these peptide arrays (SPOT - technology) is the high number of oligopeptide probes that can be tested in parallel. Detailed protocols for peptide synthesis, and the analysis of protein-protein, protein-DNA interactions as well as epitope mapping are presented in this manual. It is ideally suited not only for basic research laboratories but also for diagnostic and therapeutic applications since many diseases are related to dysfunctions in protein recognition and binding.
Reviews
From the reviews:
"In this book the authors provide a detailed overview of the technology, including insights into basic chemistry and established applications. All protocols have been written by experienced researchers in SPOT technology and have therefore been optimized for practical use. … It is ideally suited not only for basic research laboratories but also for diagnostic and therapeutic applications since many diseases are related to dysfunctions in protein recognition and binding." (New Drugs, Issue 7, October, 2002)
"Detailed protocols for peptide synthesis and the analysis of protein-protein, protein-DNA interactions as well as epitope mapping are presented in this manual. It is ideally suited not only for basic research laboratories but potentially also for diagnostic and therapeutic applications … . This book provides a detailed overview of the technology, including insights into the basic chemistry and established applications. ... The book is suitable for researchers in basic research and in therapeutic applications as well as for advanced students." (Biotech International, Vol. 15 (2), 2003)
Editors and Affiliations
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Forschungsstelle Hantaviren, Heidelberger Akademie der Wissenschaften, Germany
Joachim Koch
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Institut für Molekulare Genetik, Universität Heidelberg, Germany
Michael Mahler
Bibliographic Information
Book Title: Peptide Arrays on Membrane Supports
Book Subtitle: Synthesis and Applications
Editors: Joachim Koch, Michael Mahler
Series Title: Springer Lab Manuals
DOI: https://doi.org/10.1007/978-3-662-09229-3
Publisher: Springer Berlin, Heidelberg
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eBook Packages: Springer Book Archive
Copyright Information: Springer-Verlag Berlin Heidelberg 2002
Softcover ISBN: 978-3-540-42532-8Published: 28 February 2002
eBook ISBN: 978-3-662-09229-3Published: 09 March 2013
Edition Number: 1
Number of Pages: X, 173
Number of Illustrations: 23 b/w illustrations
Topics: Biochemistry, general, Molecular Medicine, Pharmacology/Toxicology