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Advances in Experimental Medicine and Biology

Advanced Technologies for Protein Complex Production and Characterization

Editors: Vega, M. Cristina (Ed.)

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  • Provides in-depth coverage of expression systems tailored for the production of multisubunit complexesGives extensive practical advice on how to design and conduct protein complex production experiments
  • Offers insightful discussions on characterization of purified protein complexes
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eBook 142,79 €
price for Spain (gross)
  • ISBN 978-3-319-27216-0
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 176,79 €
price for Spain (gross)
  • ISBN 978-3-319-27214-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
Softcover 176,79 €
price for Spain (gross)
  • ISBN 978-3-319-80094-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
About this book

This book presents advanced expression technologies for the production of protein complexes. Since complexes lie at the heart of modern biology, the expression, purification, and characterization of large amounts of high-quality protein complexes is crucial for the fields of biomedicine, biotechnology, and structural biology. From co-expression in E. coli, yeast, mammalian and insect cells to complex reconstitution from individual subunits, this book offers useful insights and guidance for successful protein expressionists.

Across several sections readers will discover existing opportunities for the production of protein complexes in bacterial systems (including membrane proteins and cell-free co-expression), methylotrophic and non-methylotrophic yeasts, protozoa (Leishmania terantolae and Dictyostelium discoideum), baculovirus-infected insect cells, mammalian cells, plants and algae. Complex reconstitution from individually purified subunits or subcomplexes is discussed as a complementary strategy. A last section introduces briefly some of the biophysical and structural characterization techniques for macromolecular complexes using state-of-the-art solution scattering and nuclear magnetic resonance.

This work is a guided tour over some of the most powerful and successful protein expression technologies, with a focus on co-expression and high-throughput applications. It is addressed to everyone interested in the production and characterization of macromolecular complexes, from university students who want an accessible description of the major co-expression systems to researchers in biomedicine and the life sciences seeking for an up-to-date survey of available technologies.

About the authors

Dr. M. Cristina Vega is a group leader at the Chemical and Physical Biology Department of the Center for Biological Research (CIB-CSIC) in Madrid, Spain. With substantial experience in macromolecular X-ray crystallography of protein complexes with biomedical relevance, she designs eukaryotic, yeast-based expression platforms tailored to protein complexes with a focus on cost-efficiency and improved performance. 

Table of contents (22 chapters)

Table of contents (22 chapters)
  • Protein Complex Production from the Drug Discovery Standpoint

    Moarefi, Ismail

    Pages 3-13

  • Choose a Suitable Expression Host: A Survey of Available Protein Production Platforms

    Fernández, Francisco J. (et al.)

    Pages 15-24

  • ACEMBL Tool-Kits for High-Throughput Multigene Delivery and Expression in Prokaryotic and Eukaryotic Hosts

    Nie, Yan (et al.)

    Pages 27-42

  • Complex Reconstitution and Characterization by Combining Co-expression Techniques in Escherichia coli with High-Throughput

    Vincentelli, Renaud (et al.)

    Pages 43-58

  • Membrane Protein Production in E. coli for Applications in Drug Discovery

    Snijder, Harm Jan (Arjan) (et al.)

    Pages 59-77

Buy this book

eBook 142,79 €
price for Spain (gross)
  • ISBN 978-3-319-27216-0
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 176,79 €
price for Spain (gross)
  • ISBN 978-3-319-27214-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
Softcover 176,79 €
price for Spain (gross)
  • ISBN 978-3-319-80094-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
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Bibliographic Information

Bibliographic Information
Book Title
Advanced Technologies for Protein Complex Production and Characterization
Editors
  • M. Cristina Vega
Series Title
Advances in Experimental Medicine and Biology
Series Volume
896
Copyright
2016
Publisher
Springer International Publishing
Copyright Holder
Springer International Publishing Switzerland
eBook ISBN
978-3-319-27216-0
DOI
10.1007/978-3-319-27216-0
Hardcover ISBN
978-3-319-27214-6
Softcover ISBN
978-3-319-80094-3
Series ISSN
0065-2598
Edition Number
1
Number of Pages
XIII, 382
Number of Illustrations
16 b/w illustrations, 71 illustrations in colour
Topics