Three Dimensional Human Organotypic Models for Biomedical Research

1. Front Matter

   Pages i-viii

   PDF

2. Three Dimensional Human Organotypic Models for Studying Infectious Diseases

   1. Front Matter

      Pages 1-1

      PDF

   2. Human Three-Dimensional Models for Studying Skin Pathogens

      • Elena Boero, Malgorzata Ewa Mnich, Andrea Guido Oreste Manetti, Elisabetta Soldaini, Luca Grimaldi, Fabio Bagnoli

      Pages 3-27

   3. Human Organotypic Respiratory Models

      • Mattias Svensson, Puran Chen

      Pages 29-54

   4. Human Gastrointestinal Organoid Models for Studying Microbial Disease and Cancer

      • Jayati Chakrabarti, Martha B. Dua-Awereh, Loryn Holokai, Yana Zavros

      Pages 55-75

   5. Human Organotypic Models for Anti-infective Research

      • Astrid Hendriks, Ana Rita Cruz, Elisabetta Soldaini, Andrea Guido Oreste Manetti, Fabio Bagnoli

      Pages 77-99Open Access

3. Three Dimensional Human Organotypic Models for Studying Non-infectious Diseases

   1. Front Matter

      Pages 101-101

      PDF

   2. Progress on Reconstructed Human Skin Models for Allergy Research and Identifying Contact Sensitizers

      • Charlotte Rodrigues Neves, Susan Gibbs

      Pages 103-129Open Access

   3. Cancer Explant Models

      • Christian T. Stackhouse, George Yancey Gillespie, Christopher D. Willey

      Pages 131-160

   4. Organotypic Models of Lung Cancer

      • Anna Pomerenke

      Pages 161-181

   5. Pancreatic Cancer Organotypic Models

      • Abigail Coetzee, Richard Grose, Hemant Kocher

      Pages 183-198

   6. Breast Organotypic Cancer Models

      • Pilar Carranza-Rosales, Nancy Elena Guzmán-Delgado, Irma Edith Carranza-Torres, Ezequiel Viveros-Valdez, Javier Morán-Martínez

      Pages 199-223

   7. Preclinical Organotypic Models for the Assessment of Novel Cancer Therapeutics and Treatment

      • Carol Ward, James Meehan, Mark Gray, Ian H. Kunkler, Simon P. Langdon, Alan Murray et al.

      Pages 225-246

   8. Overview and Comparison of Intestinal Organotypic Models, Intestinal Cells, and Intestinal Explants Used for Toxicity Studies

      • Marc Maresca, Philippe Pinton, El Hassan Ajandouz, Sandrine Menard, Laurent Ferrier, Isabelle P. Oswald

      Pages 247-264

   9. Correction to: Human Organotypic Models for Anti-infective Research

      • Astrid Hendriks, Ana Rita Cruz, Elisabetta Soldaini, Andrea Guido Oreste Manetti, Fabio Bagnoli

      Pages 265-265

This edited volume discusses the application of very diverse human organotypic models in major areas of biomedical research. The authors lay a main focus on infectious diseases, cancer, allergies, as well as drug/vaccine discovery and toxicology studies. Representing a valid alternative to laboratory animals, these models are relevant for most areas of translational research. As the contemporary research shows, many human tissues can today be cultivated in vitro and used for several research objectives.
This book provides an unprecedented overview of recent developments in an exciting field of research methodology. It is a reference guide for scientists in both academia and industry. Readers can update their knowledge and get hands-on recommendations on how to set up an organotypic model in their lab.

Chapters 'Progress on Reconstructed Human Skin Models for Allergy Research and Identifying Contact Sensitizers' and 'Human Organotypic Models for Anti-infective Research' of this book are available open access under a CC BY 4.0 license at link.springer.com.

• cutaneous infections
• skin equivalents
• human skin model
• skin animal model
• in vitro skin model
• in vivo skin model
• Cell culture

• GSK Vaccines Research, Siena, Italy

  Fabio Bagnoli, Rino Rappuoli

Fabio Bagnoli is discovery project leader of research & development programs at GSK. Research conducted by Fabio led to the filing of several patents as well as publications on various human pathogens published in important peer-reviewed journals. He has served as editor of several publications and is the coordinator of an industrial-academic effort on human organotypic models. He holds a PhD in Molecular and Cellular Microbiology from the University of Padova and conducted post-doctoral studies at Stanford University under the guidance of Dr. Stanley Falkow and Dr. Manuel Amieva.



Rino Rappuoli is Chief Scientist and Head External R&D at GSK Vaccines, based in Siena, Italy and Professor of Vaccinology at Imperial College, London. Prior positions: head of Vaccine R&D at Novartis, CSO of Chiron Corporation, head for R&D at Sclavo.

He earned his PhD in Biological Sciences at the University of Siena, Italy, and was visiting scientist at Rockefeller University and Harvard Medical School.

He is elected member of US National Academy of Sciences (NAS), the European Molecular Biology Organization (EMBO), and the Royal Society of London. Awards received: Gold Medal by the Italian President, Albert B Sabin Gold Medal, Canada Gairdner International Award, European Inventor Award for Lifetime Achievement, and Robert Koch Award. He was nominated third most influential person worldwide in the field of vaccines (Terrapin).   He has published more than 700 works in peer-reviewed journals.

He introduced novel scientific concepts: genetic detoxification; cellular microbiology; reverse vaccinology; pangenome. Developed licensed vaccines: acellular pertussis containing a non-toxic mutant of pertussis toxin; first conjugate vaccine against meningococcus C; MF59, the first vaccine adjuvant after aluminium salts; meningococcus B; CRM 197 used as carrier in many conjugate vaccines.

Dr. Rappuoli is among theworld scientific leaders dedicated to the sustainability of global health.

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