Springer Theses

15N Tracing of Microbial Assimilation, Partitioning and Transport of Fertilisers in Grassland Soils

Authors: Charteris, Alice Fiona

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  • Nominated as an outstanding PhD thesis by the University of Bristol, UK
  • Presents an extremely thorough and well-referenced introduction to sustainable soil nitrogen management
  • Provides detailed information on the compound-specific 15N amino acid stable isotope probing method
  • Describes the first experimental demonstrations of differential processing of N-fertilisers using soil microbes
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eBook 83,29 €
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  • ISBN 978-3-030-31057-8
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Hardcover 145,59 €
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  • ISBN 978-3-030-31056-1
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About this book

This book presents innovative research on soil nitrogen cycling and nitrate leaching with a view to improving soil management and fertiliser nitrogen use efficiency and reducing nitrogen leaching losses. In this regard, nitrogen-15 (15N)-labelled fertiliser was used as a biochemical and physical stable isotope tracer in laboratory and field experiments. The major outcome of the research was the development, validation and application of a new compound-specific amino acid 15N stable isotope probing method for assessing the assimilation of fertiliser nitrogen by soil microbial biomass. 

The novelty of the method lies in its tracing of incorporated nitrogen into newly biosynthesised microbial protein in time-course experiments using gas chromatography-combustion-isotope ratio mass spectrometry. The approach provides previously unattainable insights into the microbial processing of different nitrogen fertilisers in different soils. Further, it identifies the mechanistic link between molecular-scale processes and observations of field-scale fertiliser nitrogen immobilisation studies. The method and the results presented here will have far-reaching implications for the development of enhanced recommendations concerning farm-based soil management practices for increasing soil productivity and reducing nitrogen losses, which is essential to minimising environmental impacts.


About the authors

Alice received her B.Sc. in Chemistry from the University of Bristol. In the latter years of her degree she became fascinated by how organic chemistry and stable isotopes could be combined to probe environmental processes. This led her to pursue a Ph.D., in collaboration with Wessex Water, the goal of which was to help mitigate nitrogen fertiliser pollution problems in drinking water supplies. After completing her Ph.D., Alice pursued postdoctoral research at Rothamsted Research, North Wyke, where she was part of a NERC-funded project investigating nitrogen cycling and gaseous nitrogen losses from sheep urine patches on upland soils.

Table of contents (7 chapters)

Table of contents (7 chapters)
  • Introduction

    Pages 1-33

    Charteris, Alice Fiona

  • Sites, Sampling, Materials and Methods

    Pages 35-56

    Charteris, Alice Fiona

  • Compound-Specific Amino Acid 15N Stable Isotope Probing of Nitrogen Assimilation by the Soil Microbial Biomass Using Gas Chromatography-Combustion-Isotope Ratio Mass Spectrometry

    Pages 57-78

    Charteris, Alice Fiona

  • Biosynthetic Routing, Rates and Extents of Microbial Fertiliser Nitrogen Assimilation in Two Grazed Grassland Soils

    Pages 79-151

    Charteris, Alice Fiona

  • Microbial Fertiliser Nitrogen Assimilation in the Field as Compared with the Laboratory Incubation Experiments

    Pages 153-188

    Charteris, Alice Fiona

Buy this book

eBook 83,29 €
price for Spain (gross)
  • ISBN 978-3-030-31057-8
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 145,59 €
price for Spain (gross)
  • ISBN 978-3-030-31056-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
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Bibliographic Information

Bibliographic Information
Book Title
15N Tracing of Microbial Assimilation, Partitioning and Transport of Fertilisers in Grassland Soils
Authors
Series Title
Springer Theses
Copyright
2019
Publisher
Springer International Publishing
Copyright Holder
Springer Nature Switzerland AG
eBook ISBN
978-3-030-31057-8
DOI
10.1007/978-3-030-31057-8
Hardcover ISBN
978-3-030-31056-1
Series ISSN
2190-5053
Edition Number
1
Number of Pages
XXXV, 261
Topics