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Biological Aging

Methods and Protocols

  • Book
  • © 2013

Overview

Editors:
  1. Trygve O. Tollefsbol

    1. Dept. Biology, University of Alabama, Birmingham Dept. Biology, Birmingham, USA

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  • Focuses on contemporary approaches and established protocols to study aging cell culture
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1048)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Cell Senescence Culturing Methods

    • Huaping Chen, Yuanyuan Li, Trygve O. Tollefsbol
    Pages 1-10

  3. Digital Image Analysis of Cells Stained with the Senescence-Associated β-Galactosidase Assay

    • Liran I. Shlush, Sara Selig
    Pages 11-18

  4. Analysis of Biomarkers of Caloric Restriction in Aging Cells

    • Yuanyuan Li, Trygve O. Tollefsbol
    Pages 19-29

  5. Cell Sorting of Young and Senescent Cells

    • Graeme Hewitt, Thomas von Zglinicki, João F. Passos
    Pages 31-47

  6. Studying the Replicative Life Span of Yeast Cells

    • David A. Sinclair
    Pages 49-63

  7. Methods for Creating Mutations in C. elegans That Extend Lifespan

    • Dayong Wang, Min Cao, Jessica Dinh, Yuqing Dong
    Pages 65-75

  8. Aging Studies in Drosophila Melanogaster

    • Yaning Sun, Jason Yolitz, Cecilia Wang, Edward Spangler, Ming Zhan, Sige Zou
    Pages 77-93

  9. The Use of Calorie Restriction Mimetics to Study Aging

    • Munehiro Kitada, Daisuke Koya
    Pages 95-107

  10. Using Somatic-Cell Nuclear Transfer to Study Aging

    • Satoshi Kishigami, Ah Reum Lee, Teruhiko Wakayama
    Pages 109-126

  11. Induction of Cellular Senescence by Oncogenic RAS

    • Romi Gupta, Narendra Wajapeyee
    Pages 127-133

  12. Methods of Cellular Senescence Induction Using Oxidative Stress

    • Zhe Wang, Dandan Wei, Hengyi Xiao
    Pages 135-144

  13. Methods of Testing Pharmacological Drugs Effects on Aging and Life Span in Mice

    • Vladimir N. Anisimov, Irina G. Popovich, Mark A. Zabezhinski
    Pages 145-160

  14. Mitochondria-Targeted Antiaging Gene Therapy with Adeno-associated Viral Vectors

    • Dejia Li, Dongsheng Duan
    Pages 161-180

  15. Real-Time Bioluminescence Functional Imaging for Monitoring Tissue Formation and Regeneration

    • Nadav Bleich Kimelman, Ilan Kallai, Dmitriy Sheyn, Wafa Tawackoli, Zulma Gazit, Gadi Pelled et al.
    Pages 181-193

  16. Exometabolomic Mapping of Caenorhabditis elegans: A Tool to Noninvasively Investigate Aging

    • Robert J. Mishur, Jeffrey A. Butler, Shane L. Rea
    Pages 195-213

  17. Detecting Polymorphisms in Human Longevity Studies: HLA Typing and SNP Genotyping by Amplicon Sequencing

    • Gilberto Vargas-Alarcón, Carmina Flores-Domínguez
    Pages 215-228

  18. Gel Electrophoresis-Based Proteomics of Senescent Tissues

    • Steven Carberry, Kay Ohlendieck
    Pages 229-246

  19. Single-Cell Semiconductor Sequencing

    • Andrea B. Kohn, Tatiana P. Moroz, Jeffrey P. Barnes, Mandy Netherton, Leonid L. Moroz
    Pages 247-284

  20. Application of DNA Microarray Technology to Gerontological Studies

    • Kiyoshi Masuda, Yuki Kuwano, Kensei Nishida, Kazuhito Rokutan
    Pages 285-308
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Keywords

About this book

The second edition of Biological Aging:  Methods and Protocols expands upon the previous edition with current, detailed, useful and promising methods currently available to study aging. With new chapters on protocols that detail aging cell culture as well as many more contemporary approaches such as nuclear transfer, microarray and proteomics technologies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

 

Authoritative and practical, Biological Aging:  Methods and Protocols, Second Edition details the most recent breakthroughs in technology that have served to advance the study of aging.

Editors and Affiliations

  • Dept. Biology, University of Alabama, Birmingham Dept. Biology, Birmingham, USA

    Trygve O. Tollefsbol

Bibliographic Information

  • Book Title: Biological Aging

  • Book Subtitle: Methods and Protocols

  • Editors: Trygve O. Tollefsbol

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-556-9

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, New York 2013

  • Hardcover ISBN: 978-1-62703-555-2Published: 09 August 2013

  • Softcover ISBN: 978-1-4939-6313-3Published: 30 April 2017

  • eBook ISBN: 978-1-62703-556-9Published: 13 August 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 354

  • Number of Illustrations: 19 b/w illustrations, 35 illustrations in colour

  • Topics: Cell Biology, Cell Cycle Analysis, Apoptosis

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