Methods in Molecular Biology

Imaging Gene Expression

Methods and Protocols

Editors: Shav-Tal, Yaron (Ed.)

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  • Includes cutting-edge methods and protocols exploring the imaging of cellular aspects, tissues, and complete living organisms 
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook 93,08 €
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  • ISBN 978-1-62703-526-2
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Hardcover 114,39 €
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  • ISBN 978-1-62703-525-5
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Softcover 114,39 €
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  • ISBN 978-1-4939-6312-6
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About this book

As imaging technologies and approaches have evolved, the scope of certain imaging techniques has moved far beyond the production of purely illustrative images or appealing time-lapse movies to providing the scientist with a rich range of ways to measure and quantify the biological process and outcome of gene expression.  In Imaging Gene Expression: Methods and Protocols, expert authors offer up-to-date approaches and protocols that scientists in the field have developed, which would benefit the broader scientific community. Divided in three convenient parts, this detailed book covers the output of a gene, namely the RNA molecules that are transcribed from the gene and the way by which these molecules can be tracked or quantified in fixed or living cells, protocols that focus on the gene, DNA, or chromatin, as well as a variety of ways by which nuclear processes intertwined with gene expression can be followed and quantified in living cells as well as approaches for studying several sub-nuclear structures found in eukaryotic cells.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective subjects, lists of materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and up-to-date, Imaging Gene Expression: Methods and Protocols will serve researchers working toward imaging in the context of complete organisms.

Table of contents (23 chapters)

Table of contents (23 chapters)
  • High-Throughput Fluorescence-Based Screen to Identify Factors Involved in Nuclear Receptor Recruitment to Response Elements

    Pages 3-12

    Miranda, Tina B. (et al.)

  • Live-Cell Imaging Combined with Immunofluorescence, RNA, or DNA FISH to Study the Nuclear Dynamics and Expression of the X-Inactivation Center

    Pages 13-31

    Pollex, Tim (et al.)

  • Single-Molecule Resolution Fluorescent In Situ Hybridization (smFISH) in the Yeast S. cerevisiae

    Pages 33-46

    Rahman, Samir (et al.)

  • Measuring Transcription Dynamics in Living Cells Using Fluctuation Analysis

    Pages 47-60

    Ferguson, Matthew L. (et al.)

  • Tracking Nuclear Poly(A) RNA Movement Within and Among Speckle Nuclear Bodies and the Surrounding Nucleoplasm

    Pages 61-71

    Politz, Joan C. Ritland (et al.)

Buy this book

eBook 93,08 €
price for Spain (gross)
  • ISBN 978-1-62703-526-2
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 114,39 €
price for Spain (gross)
  • ISBN 978-1-62703-525-5
  • with online files
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
  • The final prices may differ from the prices shown due to specifics of VAT rules
Softcover 114,39 €
price for Spain (gross)
  • ISBN 978-1-4939-6312-6
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
  • The final prices may differ from the prices shown due to specifics of VAT rules
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Bibliographic Information

Bibliographic Information
Book Title
Imaging Gene Expression
Book Subtitle
Methods and Protocols
Editors
  • Yaron Shav-Tal
Series Title
Methods in Molecular Biology
Series Volume
1042
Copyright
2013
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-62703-526-2
DOI
10.1007/978-1-62703-526-2
Hardcover ISBN
978-1-62703-525-5
Softcover ISBN
978-1-4939-6312-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIII, 368
Number of Illustrations
18 b/w illustrations, 36 illustrations in colour
Topics