Mammalian Oocyte Regulation
Methods and Protocols
Editors: Homer, Hayden A. (Ed.)
Free Preview- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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- About this book
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Mammalian oocytes occupy a critical nexus in reproduction as they not only contribute half the genetic makeup of the embryo but also provide virtually all of the cytoplasmic building blocks required for sustaining embryogenesis. The journey that transforms a primordial germ cell into a mature oocyte (or egg) capable of fertilisation and embryonic development is of unrivalled complexity. The end-product of this remarkable developmental process is the “mature” egg, now replete with macromolecules and organelles, and poised to support embryonic development if fertilisation should occur. Given the importance of the oocyte for embryonic development, it is of no surprise that the oocyte holds centre stage in fertility clinics and greatly influences the outcome of assisted reproductive treatments (ARTs). Mammalian Oocyte Regulation: Methods and Protocols provides a highly diverse compendium of detailed methodologies - primarily focusing on the murine model but also including chapters on human oocytes - for enabling researchers to interrogate every aspect of mammalian oocyte development including recombination, meiotic maturation, oocyte substrate uptake, chromosomal segregation and fertilisation. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Mammalian Oocyte Regulation: Methods and Protocols serves as an ideal guide to novice oocyte researchers, experts who wish to add another dimension to their established portfolio, as well as the wider research community.
- Table of contents (21 chapters)
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Studying Recombination in Mouse Oocytes
Pages 1-18
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Analysis of Recombinants in Female Mouse Meiosis
Pages 19-45
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Studying Meiosis-Specific Cohesins in Mouse Embryonic Oocytes
Pages 47-57
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Immunohistochemical Approaches to the Study of Human Fetal Ovarian Development
Pages 59-75
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Protein Kinase Assays for Measuring MPF and MAPK Activities in Mouse and Rat Oocytes and Early Embryos
Pages 77-89
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Table of contents (21 chapters)
- Download Preface 1 PDF (91.8 KB)
- Download Sample pages 1 PDF (720.3 KB)
- Download Table of contents PDF (91.9 KB)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Mammalian Oocyte Regulation
- Book Subtitle
- Methods and Protocols
- Editors
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- Hayden A. Homer
- Series Title
- Methods in Molecular Biology
- Series Volume
- 957
- Copyright
- 2013
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC
- eBook ISBN
- 978-1-62703-191-2
- DOI
- 10.1007/978-1-62703-191-2
- Hardcover ISBN
- 978-1-62703-190-5
- Softcover ISBN
- 978-1-4939-5940-2
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XI, 311
- Topics
