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  • © 2013

Retinal Degeneration

Methods and Protocols

  • Features a comprehensive overview and detailed lab protocols relevant to retinal homeostasis and disease
  • Provides step-by-sep detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 935)

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-xiv
  2. Human Genetics

    1. Front Matter

      Pages 1-1
  3. Human Genetics

    1. Identification and Analysis of Inherited Retinal Disease Genes

      • Kornelia Neveling, Anneke I. den Hollander, Frans P. M. Cremers, Rob W. J. Collin
      Pages 3-23
  4. Mouse Models

    1. Front Matter

      Pages 25-25
  5. Mouse Models

    1. Retinal Fundus Imaging in Mouse Models of Retinal Diseases

      • Anne F. Alex, Peter Heiduschka, Nicole Eter
      Pages 41-67
    2. Functional Phenotyping of Mouse Models with ERG

      • Naoyuki Tanimoto, Vithiyanjali Sothilingam, Mathias W. Seeliger
      Pages 69-78
    3. Phenotyping of Mouse Models with OCT

      • M. Dominik Fischer, Ahmad Zhour, Christoph J. Kernstock
      Pages 79-85
    4. Light Damage as a Model of Retinal Degeneration

      • Christian Grimm, Charlotte E. Remé
      Pages 87-97
  6. Other Animal Models

    1. Front Matter

      Pages 111-111
  7. Other Animal Models

    1. Generation of Transgenic X. laevis Models of Retinal Degeneration

      • Beatrice M. Tam, Christine C.-L. Lai, Zusheng Zong, Orson L. Moritz
      Pages 113-125
    2. Analysis of Photoreceptor Degeneration in the Zebrafish Danio rerio

      • Holger Dill, Bastian Linder, Anja Hirmer, Utz Fischer
      Pages 127-137
    3. Analysis of Optokinetic Response in Zebrafish by Computer-Based Eye Tracking

      • Sabina P. Huber-Reggi, Kaspar P. Mueller, Stephan C. F. Neuhauss
      Pages 139-160
  8. In Situ Analyses

    1. Front Matter

      Pages 183-183
    2. Cell-Specific Markers for the Identification of Retinal Cells by Immunofluorescence Microscopy

      • Christiana L. Cheng, Hidayat Djajadi, Robert S. Molday
      Pages 185-199
    3. A Method of Horizontally Sliced Preparation of the Retina

      • Ryosuke Enoki, Amane Koizumi
      Pages 201-205
    4. Detection of DNA Fragmentation in Retinal Apoptosis by TUNEL

      • Francesca Doonan, Thomas G. Cotter
      Pages 207-213

About this book

Over the past decades, knowledge about the cellular and molecular basis underlying the visual process has remarkably increased. In Retinal Degeneration: Methods and Protocols, expert researchers in the field provide a guide of relevant and state-of-the-art methods for studying retinal homeostasis and disease. These include methods and techniques for addressing cell culture systems and animal models of disease, their generation, their phenotypic and molecular characterization as well as their use in therapeutic approaches to the retina. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Retinal Degeneration: Methods and Protocols aids scientists in continuing to study the  cutting-edge techniques of retinal cell biology in health and disease.

Reviews

From the book reviews:

“This step-by-step guide to studying retinal cell biology in health and disease covers a large spectrum of techniques and animal model species. … Certainly, this book is for basic scientists. It is a great tool for those based in laboratories, and researchers at all levels can benefit from the basics to key tips on troubleshooting cutting-edge techniques and avoiding pitfalls. … This book is great in simplifying new cutting-edge techniques in studying retinal degeneration.” (Ayham Skaf, Doody’s Book Reviews, May, 2013)

Editors and Affiliations

  • , Institute of Human Genetics, University of Regensburg, Regensburg, Germany

    Bernhard H.F. Weber

  • INSTITUTE OF HUMAN GENETICS, INSTITUTE OF HUMAN GENETICS, 93053 REGENSBURG, Germany

    THOMAS LANGMANN

Bibliographic Information

  • Book Title: Retinal Degeneration

  • Book Subtitle: Methods and Protocols

  • Editors: Bernhard H.F. Weber, THOMAS LANGMANN

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-080-9

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2013

  • Hardcover ISBN: 978-1-62703-079-3Published: 13 November 2012

  • Softcover ISBN: 978-1-4939-5969-3Published: 23 August 2016

  • eBook ISBN: 978-1-62703-080-9Published: 13 November 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 384

  • Number of Illustrations: 50 b/w illustrations, 48 illustrations in colour

  • Topics: Molecular Medicine, Ophthalmology

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access