Overview
- Covers isolation characterization of numerous human embryonic stem cells as well as essential protocols in HES characterization
- Features detailed, cutting-edge methods and protocols
- Includes expert tips and key implementation advice for successful implementation
Part of the book series: Methods in Molecular Biology (MIMB, volume 873)
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Table of contents (20 protocols)
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Front Matter
Keywords
About this book
Considerable advances have taken place since the initial isolation and characterization of human embryonic stem (HES) cells; however, significant challenges remain before their potential for restoration and regeneration processes in patients can be realized. Understanding the diversity amongst HES cell lines and realizing the ability to isolate lines with robust differentiation potential remain difficult. In the Human Embryonic Stem Cells Handbook, experts in the field provide an assortment of protocols that have been used by various laboratories around the world so as to allow both novices and experienced investigators to compare and contrast different approaches to HES cell isolation and characterization with the hope that, from these protocols, researchers might standardize approaches for HES cell biology. Written in the Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.
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Authoritative and accessible, Human Embryonic Stem Cells Handbook serves as a valuable reference for scientists pursuing this vital field and its enormous potential.Editors and Affiliations
Bibliographic Information
Book Title: Human Embryonic Stem Cells Handbook
Editors: Kursad Turksen
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-61779-794-1
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2012
Hardcover ISBN: 978-1-61779-793-4Published: 20 April 2012
Softcover ISBN: 978-1-4939-6262-4Published: 23 August 2016
eBook ISBN: 978-1-61779-794-1Published: 23 April 2012
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 313
Number of Illustrations: 36 b/w illustrations, 17 illustrations in colour
Topics: Cell Biology, Stem Cells