Methods in Molecular Biology

Amyloid Proteins

Methods and Protocols

Editors: Sigurdsson, Einar M., Calero, Miguel, Gasset, María (Eds.)

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About this book

Amyloid diseases are characterized by the deposition of insoluble fibrous amyloid proteins. The word “amyloid” indicates a starch-like compound, and though a misnomer, continues to be the accepted term for this group of protein conformational disorders. The second edition  of Amyloid Proteins expands upon the previous edition with current, detailed protocols for the preparation of amyloid and its precursors, specific analytical methods for studying these proteins, cell culture models and assays for production of amyloid proteins, and protocols for amyloid extraction from tissue, its detection in vitro and in vivo, as well as nontransgenic methods for developing amyloid mouse models. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Amyloid Proteins, Second Edition seeks to aid scientists in the amyloid field to establish new techniques in their laboratories.

Authoritative and practical, Amyloid Proteins, Second Edition seeks to aid scientists in the amyloid field to establish new techniques in their laboratories.

Table of contents (35 chapters)

Table of contents (35 chapters)
  • Rapid Generation of Dityrosine Cross-linked Aβ Oligomers via Cu-Redox Cycling

    Pages 3-10

    Gunn, Adam P. (et al.)

  • Application of Photochemical Cross-linking to the Study of Oligomerization of Amyloidogenic Proteins

    Pages 11-21

    Lopes, Dahabada H. J. (et al.)

  • Preparation of Stable Amyloid β-Protein Oligomers of Defined Assembly Order

    Pages 23-31

    Rosensweig, Clark (et al.)

  • Purification and Fibrillation of Full-Length Recombinant PrP

    Pages 33-52

    Makarava, Natallia (et al.)

  • Featuring Amyloids with Fourier Transform Infrared and Circular Dichroism Spectroscopies

    Pages 53-68

    Calero, Miguel (et al.)

Buy this book

eBook 139,09 €
price for Spain (gross)
  • ISBN 978-1-61779-551-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 176,79 €
price for Spain (gross)
  • ISBN 978-1-61779-550-3
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
  • The final prices may differ from the prices shown due to specifics of VAT rules
Softcover 171,59 €
price for Spain (gross)
  • ISBN 978-1-4939-5623-4
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
  • The final prices may differ from the prices shown due to specifics of VAT rules
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Bibliographic Information

Bibliographic Information
Book Title
Amyloid Proteins
Book Subtitle
Methods and Protocols
Editors
  • Einar M. Sigurdsson
  • Miguel Calero
  • María Gasset
Series Title
Methods in Molecular Biology
Series Volume
849
Copyright
2012
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-551-0
DOI
10.1007/978-1-61779-551-0
Hardcover ISBN
978-1-61779-550-3
Softcover ISBN
978-1-4939-5623-4
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XV, 548
Number of Illustrations
69 b/w illustrations, 46 illustrations in colour
Topics