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Amyloid Proteins

Methods and Protocols

  • Book
  • © 2012

Overview

Editors:
  1. Einar M. Sigurdsson

    1. School of Medicine, Dept. Psychiatry, New York University, New York, USA

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  2. Miguel Calero

    1. Centro Nacional de Microbiología, Instituto de Salud Carlos III, Madrid, Spain

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    You can also search for this editor in PubMed   Google Scholar

  3. María Gasset

    1. Inst. Química Física, Rocasolano, CSIC Madrid, Madrid, Spain

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    You can also search for this editor in PubMed   Google Scholar

  • Detailed protocols for performing a particular procedure
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 849)

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Table of contents (35 protocols)

  1. Front Matter

    Pages i-xv
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  2. In Vitro Models and Assays

    1. Front Matter

      Pages 1-1
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  3. IN VITRO MODELS AND ASSAYS

    1. Rapid Generation of Dityrosine Cross-linked Aβ Oligomers via Cu-Redox Cycling

      • Adam P. Gunn, Blaine R. Roberts, Ashley I. Bush
      Pages 3-10

    2. Application of Photochemical Cross-linking to the Study of Oligomerization of Amyloidogenic Proteins

      • Dahabada H. J. Lopes, Sharmistha Sinha, Clark Rosensweig, Gal Bitan
      Pages 11-21

    3. Preparation of Stable Amyloid β-Protein Oligomers of Defined Assembly Order

      • Clark Rosensweig, Kenjiro Ono, Kazuma Murakami, Devin K. Lowenstein, Gal Bitan, David B. Teplow
      Pages 23-31

    4. Purification and Fibrillation of Full-Length Recombinant PrP

      • Natallia Makarava, Ilia V. Baskakov
      Pages 33-52

    5. Featuring Amyloids with Fourier Transform Infrared and Circular Dichroism Spectroscopies

      • Miguel Calero, María Gasset
      Pages 53-68

    6. Quasielastic Light Scattering Study of Amyloid β-Protein Fibrillogenesis

      • Aleksey Lomakin, David B. Teplow
      Pages 69-83

    7. Conformations of Microtubule-Associated Protein Tau Mapped by Fluorescence Resonance Energy Transfer

      • Sadasivam Jeganathan, Subashchandrabose Chinnathambi, Eva-Maria Mandelkow, Eckhard Mandelkow
      Pages 85-99

    8. Measuring the Kinetics of Amyloid Fibril Elongation Using Quartz Crystal Microbalances

      • Alexander K. Buell, Christopher M. Dobson, Mark E. Welland
      Pages 101-119

    9. X-Ray Fibre Diffraction Studies of Amyloid Fibrils

      • Kyle L. Morris, Louise C. Serpell
      Pages 121-135

    10. Structural Characterization of Prefibrillar Intermediates and Amyloid Fibrils by Small-Angle X-Ray Scattering

      • Annette Eva Langkilde, Bente Vestergaard
      Pages 137-155

    11. Atomic Force Fluorescence Microscopy in the Characterization of Amyloid Fibril Assembly and Oligomeric Intermediates

      • Valeriy Ostapchenko, Maria Gasset, Ilia V. Baskakov
      Pages 157-167

    12. Investigating Fibrillar Aggregates of Tau Protein by Atomic Force Microscopy

      • Susanne Wegmann, Daniel J. Muller, Eckhard Mandelkow
      Pages 169-183

    13. Structural Studies of Amyloids by Quenched Hydrogen–Deuterium Exchange by NMR

      • Marçal Vilar, Lei Wang, Roland Riek
      Pages 185-198

    14. Cyclic Amplification of Prion Protein Misfolding

      • Marcelo A. Barria, Dennisse Gonzalez-Romero, Claudio Soto
      Pages 199-212

    15. Search for Amyloid-Binding Proteins by Affinity Chromatography

      • Miguel Calero, Agueda Rostagno, Jorge Ghiso
      Pages 213-223

  4. Cell Culture Models and Assays

    1. Front Matter

      Pages 225-225
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  5. CELL CULTURE MODELS AND ASSAYS

    1. Establishing the Links Between Aβ Aggregation and Cytotoxicity In Vitro Using Biophysical Approaches

      • Asad Jan, Hilal A. Lashuel
      Pages 227-243

    2. Preparation of Cultured Human Vascular Cells

      • Ingvar H. Olafsson, Dadi Th. Vilhjalmsson, Finnbogi R. Thormodsson
      Pages 245-259
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Keywords

About this book

Amyloid diseases are characterized by the deposition of insoluble fibrous amyloid proteins. The word “amyloid” indicates a starch-like compound, and though a misnomer, continues to be the accepted term for this group of protein conformational disorders. The second edition  of Amyloid Proteins expands upon the previous edition with current, detailed protocols for the preparation of amyloid and its precursors, specific analytical methods for studying these proteins, cell culture models and assays for production of amyloid proteins, and protocols for amyloid extraction from tissue, its detection in vitro and in vivo, as well as nontransgenic methods for developing amyloid mouse models. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Amyloid Proteins, Second Edition seeks to aid scientists in the amyloid field to establish new techniques in their laboratories.

Authoritative and practical, Amyloid Proteins, Second Edition seeks to aid scientists in the amyloid field to establish new techniques in their laboratories.

Editors and Affiliations

  • School of Medicine, Dept. Psychiatry, New York University, New York, USA

    Einar M. Sigurdsson

  • Centro Nacional de Microbiología, Instituto de Salud Carlos III, Madrid, Spain

    Miguel Calero

  • Inst. Química Física, Rocasolano, CSIC Madrid, Madrid, Spain

    María Gasset

Bibliographic Information

  • Book Title: Amyloid Proteins

  • Book Subtitle: Methods and Protocols

  • Editors: Einar M. Sigurdsson, Miguel Calero, María Gasset

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-551-0

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-550-3Published: 24 February 2012

  • Softcover ISBN: 978-1-4939-5623-4Published: 23 August 2016

  • eBook ISBN: 978-1-61779-551-0Published: 23 April 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XV, 548

  • Number of Illustrations: 69 b/w illustrations, 46 illustrations in colour

  • Topics: Protein Science

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