Methods in Molecular Biology

Human Cell Culture Protocols

Editors: Mitry, Ragai R., Hughes, Robin D. (Eds.)

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  • Supplies techniques that can be adapted to obtain cell cultures from diseased cells to gain understanding of the pathogenic mechanisms involved
  • Features cutting-edge methods ready for the laboratory
  • Includes expert tips and implementation advice to ensure successful results
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eBook 118,99 €
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  • ISBN 978-1-61779-367-7
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  • Immediate eBook download after purchase
Hardcover 150,79 €
price for Spain (gross)
  • ISBN 978-1-61779-366-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
Softcover 150,79 €
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  • ISBN 978-1-4939-6208-2
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  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
About this book

Human cell culture is not a new topic, but the development of new molecular techniques and reagents which can be used to investigate cell function and the responsible intracellular mechanisms make it a continuing requirement.  This third edition of Human Cell Culture Protocols expands upon the previous editions with current, detailed protocols for the isolation and culture of a range of primary cells from human tissues.  With new chapters on pancreatic cells needed for basic studies on the pathogenesis of diabetes and for their application for islet transplantation, the book also delves into protocols for hepatocytes, skin cells, lung cells, parathyroid cells, gastric cells, renal cells, adipocytes, ovarian cells, bone cells, vascular smooth muscle cells, vascular endothelial cells, regulatory T cells, blood mononuclear cells, as well as new techniques being applied to human cell culture, particularly the use of biocompatible scaffolds to grow cells, the in vitro use of laser microdissection to isolate cells from culture, and automated cell culture. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Human Cell Culture Protocols, Third Edition makes it possible for a worker with basic cell culture training, whether in the fields of cell biology, gene therapy, and cell transplantation, to prepare cell cultures of the specific cell type necessary to forward their vital research.

Table of contents (27 chapters)

  • Introduction to Cell Culture

    Philippeos, Christina (et al.)

    Pages 1-13

    Preview Buy Chapter 48,34 €
  • Isolation and Cultivation of Dermal Stem Cells that Differentiate into Functional Epidermal Melanocytes

    Li, Ling (et al.)

    Pages 15-29

    Preview Buy Chapter 48,34 €
  • Isolation, Cultivation, and Application of Human Alveolar Epithelial Cells

    Daum, Nicole (et al.)

    Pages 31-42

    Preview Buy Chapter 48,34 €
  • Culture of Parathyroid Cells

    Björklund, Peyman (et al.)

    Pages 43-53

    Preview Buy Chapter 48,34 €
  • Functional Analysis of Human Islets of Langerhans Maintained in Culture

    Persaud, Shanta J. (et al.)

    Pages 55-71

    Preview Buy Chapter 48,34 €

Buy this book

eBook 118,99 €
price for Spain (gross)
  • ISBN 978-1-61779-367-7
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 150,79 €
price for Spain (gross)
  • ISBN 978-1-61779-366-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
Softcover 150,79 €
price for Spain (gross)
  • ISBN 978-1-4939-6208-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
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Bibliographic Information

Bibliographic Information
Book Title
Human Cell Culture Protocols
Editors
  • Ragai R. Mitry
  • Robin D. Hughes
Series Title
Methods in Molecular Biology
Series Volume
806
Copyright
2012
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
Distribution Rights
Distribution rights In India: Panima Educational Book Agency, New Delhi, India
eBook ISBN
978-1-61779-367-7
DOI
10.1007/978-1-61779-367-7
Hardcover ISBN
978-1-61779-366-0
Softcover ISBN
978-1-4939-6208-2
Series ISSN
1064-3745
Edition Number
3
Number of Pages
XIV, 435
Topics