Gene Expression Profiling
Methods and Protocols
Editors: O'Driscoll, Lorraine (Ed.)
Free Preview- -Presents a broad range of available and developing techniques for the analysis of gene expression at the DNA, RNA, and protein levels
- -Features easy to follow, step-by-step procedures, ready for the lab
- -Includes vital tips and expert implementation advice
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- About this book
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Understanding gene expression and how it changes under normal and pathological conditions is essential to our understanding of the fundamentals of cell biology through to the targeted treatment of disease. In Gene Expression Profiling: Methods and Protocols, Second Edition, experts in their particular fields compile detailed protocols for a broad range of techniques, currently available and being further developed, for the analysis of gene expression at the DNA, RNA, and protein levels. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and easy-to-use, Gene Expression Profiling: Methods and Protocols, Second Edition presents a collection of clearly described and illustrated chapters, certain to be helpful to researchers in academia, in hospitals, and in industry who are interested in applying techniques, whether basic or advanced, for the analysis of gene expression. - Reviews
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From the reviews of the second edition:
“This beautifully illustrated book on gene expression profiling provides detailed protocols for a broad range of techniques … for the analysis of gene expression at the DNA, RNA, and protein levels. … Although written for researchers, the book will be helpful for graduate students and others interested in learning about gene expression. … This is a valuable resource on methods and protocols necessary to understand the changes in gene expression in normal and pathological states in pursuit of better treatments.” (Omer Iqbal, Doody’s Book Reviews, April, 2012)
- Table of contents (14 chapters)
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Real-Time PCR and Multiplex Approaches
Pages 1-13
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Reverse-Transcriptase Polymerase Chain Reaction to Detect Extracellular mRNAs
Pages 15-25
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Microarray Analysis of mRNAs: Experimental Design and Data Analysis Fundamentals
Pages 27-40
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Software and Tools for Microarray Data Analysis
Pages 41-53
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Analysis of Gene Expression as Relevant to Cancer Cells and Circulating Tumour Cells
Pages 55-75
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Table of contents (14 chapters)
- Download Preface 1 PDF (35.6 KB)
- Download Sample pages 1 PDF (238.4 KB)
- Download Table of contents PDF (34 KB)
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Bibliographic Information
- Bibliographic Information
-
- Book Title
- Gene Expression Profiling
- Book Subtitle
- Methods and Protocols
- Editors
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- Lorraine O'Driscoll
- Series Title
- Methods in Molecular Biology
- Series Volume
- 784
- Copyright
- 2011
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC
- eBook ISBN
- 978-1-61779-289-2
- DOI
- 10.1007/978-1-61779-289-2
- Hardcover ISBN
- 978-1-61779-288-5
- Softcover ISBN
- 978-1-4939-6173-3
- Series ISSN
- 1064-3745
- Edition Number
- 2
- Number of Pages
- X, 211
- Number of Illustrations
- 22 b/w illustrations, 15 illustrations in colour
- Topics
