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Methods in Molecular Biology

Therapeutic Oligonucleotides

Methods and Protocols

Editors: Goodchild, John (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook 117,69 €
price for Spain (gross)
  • ISBN 978-1-61779-188-8
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 176,79 €
price for Spain (gross)
Softcover 150,79 €
price for Spain (gross)
About this book

The field of oligonucleotide therapeutics research is ripe with the prospect of new discoveries. In Therapeutic Oligonucleotides: Methods and Protocols, a selection of established and emerging methods for the application of oligonucleotides as therapeutics are presented, all providing the tools needed to inspire great changes in the field. Divided into twenty-one chapters, this detailed volume meticulously describes vital protocols for optimizing and improving cell uptake, such as photochemical internalization, modified cell penetrating peptides, antibody conjugates, and nanoparticles. Other chapters address quantitation of RNA therapeutics in cells, assaying gene knockdown, selecting the best target site and synthesis of various modified oligonucleotides. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Therapeutic Oligonucleotides: Methods and Protocols serves as a timely resource for both professionals and novices pursuing research in this exciting and pioneering field.

Reviews

From the reviews:

“The book is written for both professionals and novices interested in the field of oligonucleotide therapeutics. It also would be appropriate for graduate students and research scientists interested in this area. … This is an extremely useful resource that provides different ways that oligonucleotides may be applied as therapeutics. Very few books cover the field as thoroughly and as clearly as this one.” (Omer Iqbal, Doody’s Book Reviews, November, 2011)

Table of contents (21 chapters)

Table of contents (21 chapters)

Buy this book

eBook 117,69 €
price for Spain (gross)
  • ISBN 978-1-61779-188-8
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 176,79 €
price for Spain (gross)
Softcover 150,79 €
price for Spain (gross)
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Bibliographic Information

Bibliographic Information
Book Title
Therapeutic Oligonucleotides
Book Subtitle
Methods and Protocols
Editors
  • John Goodchild
Series Title
Methods in Molecular Biology
Series Volume
764
Copyright
2011
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-188-8
DOI
10.1007/978-1-61779-188-8
Hardcover ISBN
978-1-61779-187-1
Softcover ISBN
978-1-4939-5822-1
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 340
Number of Illustrations
70 b/w illustrations
Topics