Formalin-Fixed Paraffin-Embedded Tissues
Methods and Protocols
Editors: Al-Mulla, Fahd (Ed.)
Free Preview- Includes well-established and tested methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains well-organized themes and key implementation advice from the experts
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- About this book
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Presenting an area of research that intersects with and integrates diverse disciplines, including genomics, epigenetics, proteomics, and cellular biology, among others, Formalin-Fixed Paraffin-Embedded Tissues: Methods and Protocols collects contributions from expert researchers in order to provide practical guidelines to this complex study. Compiled in order to provide researchers with up-to-date methodological information pertaining to the utilization of genomic, transcriptomic, and proteomic data in diagnosis, prognosis, and tailored therapy, the ultimate aim of this volume is to decipher diseases at a molecular level. Divided into multiple convenient chapters, this detailed book covers various techniques to construct and utilize tissue arrays, it also provides detailed protocols in immunohistochemistry, immunofluorescence, fluorescent and chromogenic in situ hybridization, and ultimately introduces protocols for FFPET microdissection and nucleic acids extraction for their utilization in advanced techniques such as microarray CGH, DNA methylation and pyrosequencing. The volume also discusses FFPET research from an ethical standpoint and concludes with a chapter on novel tissue fixative. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and accessible, Formalin-Fixed Paraffin-Embedded Tissues: Methods and Protocols serves as a practical guide for scientists of all backgrounds and aims to convey the appropriate sense of fascination associated with this vital field of research.
- Reviews
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From the reviews:
“This text can be considered an introductory compendium of current methods and protocols in Molecular Biology for the use of Fixed Paraffin-Embedded Tissues that will be particularly suited to new researchers in the field who are eager to develop expertise. It is an easily readable and technically comprehensive book that would be ideal for initial experimental planning which can be complemented by other bibliographic sources and scientific journals for in-depth discussions of particular techniques and their potential applications.” (Flavia Troglio and Manuela Vecchi, Molecular Biotechnology, 2012)
- Table of contents (19 chapters)
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Regulatory and Ethical Issues on the Utilization of FFPE Tissues in Research
Pages 1-21
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Tissue Microarrays: Construction and Uses
Pages 23-35
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Standardization in Immunohistology
Pages 37-68
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Multiple Immunofluorescence Labeling of Formalin-Fixed Paraffin-Embedded Tissue
Pages 69-77
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Microwaves for Chromogenic In Situ Hybridization
Pages 79-89
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Table of contents (19 chapters)
- Download Preface 1 PDF (163 KB)
- Download Sample pages 1 PDF (1.9 MB)
- Download Table of contents PDF (162.4 KB)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Formalin-Fixed Paraffin-Embedded Tissues
- Book Subtitle
- Methods and Protocols
- Editors
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- Fahd Al-Mulla
- Series Title
- Methods in Molecular Biology
- Series Volume
- 724
- Copyright
- 2011
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC
- eBook ISBN
- 978-1-61779-055-3
- DOI
- 10.1007/978-1-61779-055-3
- Hardcover ISBN
- 978-1-61779-054-6
- Softcover ISBN
- 978-1-4939-6171-9
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XII, 312
- Topics
