Nitric Oxide
Methods and Protocols
Editors: McCarthy, Helen O., Coulter, Jonathan A. (Eds.)
Free Preview- Addresses the challenges of accurate quantification of nitric oxide in either in vitro or in vivo models with practical, step-by-step protocols
- Includes readily reproducible techniques crafted by leading experts
- Provides detailed notes sections, documenting specific information on particularly challenging aspects of the methodologies
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- About this book
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The past two decades have seen an explosion in the number of research articles relating to both the physiological and pathological responses evoked by nitric oxide generation. Despite this, accurate quantification of nitric oxide in either in vitro or in vivo models remains challenging, due to the relatively unstable nature of the molecule. In Nitric Oxide: Methods and Protocols, experts in the field provide a detailed reference manual packed with step-by-step approaches to reproduce various complex protocols within your own laboratory. Divided into two parts, the volume first covers various disparate protocols relating to the direct detection and quantification of nitric oxide, including techniques which detail how to image real time in vivo generation of nitric oxide, quantify nitric oxide production in the rat brain, and the detect ultralow levels of nitric oxide in the pM range, and the book then continues with a section on techniques designed to either inhibit or enhance nitric oxide, with an aim to achieve therapeutic gain, featuring the inhibition of the nitric oxide synthase enzymes using viral, shRNA delivery systems to prevent cardiovascular dysfunction, peripheral neuropathy, and graft rejection. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Nitric Oxide: Methods and Protocols examines numerous complex methodologies detailed in each chapter and proves to be an invaluable resource for anyone with an interest in nitric oxide research.
- Table of contents (15 chapters)
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Nitric Oxide Physiology and Pathology
Pages 1-13
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Ionizing Radiation-Induced DNA Strand Breaks and γ-H2AX Foci in Cells Exposed to Nitric Oxide
Pages 17-25
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Determination of S-Nitrosothiols in Biological Fluids by Chemiluminescence
Pages 27-37
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Measurement of Nitrite in Blood Samples Using the Ferricyanide-Based Hemoglobin Oxidation Assay
Pages 39-56
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Selective Fluorescent Activation for Bioimaging the Expression of Nitric Oxide in Cellular and In Vivo Systems
Pages 57-71
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Table of contents (15 chapters)
- Download Preface 1 PDF (34.7 KB)
- Download Sample pages 1 PDF (278.4 KB)
- Download Table of contents PDF (48.1 KB)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Nitric Oxide
- Book Subtitle
- Methods and Protocols
- Editors
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- Helen O. McCarthy
- Jonathan A. Coulter
- Series Title
- Methods in Molecular Biology
- Series Volume
- 704
- Copyright
- 2011
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC
- eBook ISBN
- 978-1-61737-964-2
- DOI
- 10.1007/978-1-61737-964-2
- Hardcover ISBN
- 978-1-61737-963-5
- Softcover ISBN
- 978-1-4939-5791-0
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XI, 226
- Topics
