Methods in Molecular Biology

Transgenic Plants

Methods and Protocols

Editors: Peña, Leandro (Ed.)

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About this book

Transgenic plants serve as essential tools for research in plant biology and are the basis of modern agricultural biotechnology. In Transgenic Plants: Methods and Protocols, leading scientists describe readily reproducible techniques they have perfected for the genetic transformation and regeneration of plants. The authors cover the most commonly used transformation systems, including Agrobacterium tumefaciens, Agrobacterium rhizogenes, particle bombardment, electroporation, floral-dip, and chloroplast transformation, as well as the regeneration of whole transgenic plants by organogenesis and somatic embryogenesis from different explant cells/tissues and from such diverse plant species as tomato, cassava, conifers, and citrus. Also described are the use of selectable and reporter markers, positive selection (for maize transformation), and the controlled excision and removal of marker genes from both nuclei and plastids once transgenic shoots have been efficiently generated. Additional techniques employ quantitative real-time PCR, reverse transcription PCR, fluorescence in situ hybridization, and thermal asymmetric interlaced PCR. Risk assessment methods offer the opportunity to study Agrobacterium persistence in plant tissues and to investigate the possibility of transgene dispersal. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and easy to use, Transgenic Plants: Methods and Protocols offers both novice and experienced researchers a cutting-edge laboratory manual covering all aspects of plant genetic transformation and transgenic crops.

Table of contents (27 chapters)

Table of contents (27 chapters)
  • Transgenic Plants: An Historical Perspective

    Pages 3-31

    Herrera-Estrella, Luis (et al.)

  • Plant Transformation: Agrobacterium-Mediated Gene Transfer

    Pages 35-46

    Dandekar, Abhaya M. (et al.)

  • Production of Hairy Root Cultures and Transgenic Plants by Agrobacterium rhizogenes-Mediated Transformation

    Pages 47-60

    Christey, Mary C. (et al.)

  • Stable Transformation of Plant Cells by Particle Bombardment/Biolistics

    Pages 61-78

    Kikkert, Julie R. (et al.)

  • Electroporation: Introduction and Expression of Transgenes in Plant Protoplasts

    Pages 79-90

    Fisk, Henry J. (et al.)

Buy this book

eBook 99,99 €
price for Spain (gross)
  • ISBN 978-1-59259-827-4
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Softcover 122,67 €
price for Spain (gross)
  • ISBN 978-1-61737-461-6
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
  • The final prices may differ from the prices shown due to specifics of VAT rules
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Bibliographic Information

Bibliographic Information
Book Title
Transgenic Plants
Book Subtitle
Methods and Protocols
Editors
  • Leandro Peña
Series Title
Methods in Molecular Biology
Series Volume
286
Copyright
2004
Publisher
Humana Press
Copyright Holder
Humana Press
Distribution Rights
Distribution rights for India: CBS Publishers, New Delhi, India
eBook ISBN
978-1-59259-827-4
DOI
10.1385/1592598277
Softcover ISBN
978-1-61737-461-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 437
Topics