Enzyme Stabilization and Immobilization
Methods and Protocols
Editors: Minteer, Shelley D. (Ed.)
Free Preview- -Provides protocols for enzyme stabilization that can be used for any enzyme of interest to the reader
- -Includes up-to-date protocols on the latest enzyme immobilization techniques
- -Provides a thorough review of methods for accurately determining enzyme activity and stability
- -Procedures are scaleable from microscale molecular biology assays to industrial bioprocessing
Buy this book
- About this book
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In the past decade, researchers have made tremendous progress in the field of enzyme stabilization, opening up new opportunities for enzymes in molecular biology and for industrial applications. In Enzyme Stabilization and Immobilization: Methods and Protocols, expert researchers explore the latest developments through detailed laboratory protocols, which address many different theories and techniques in enzyme stabilization. Chapters outline protocols for enzyme stabilization in solutions, including: liposome formation, micelle introduction, crosslinking, and additives. Secondly, the book contain protocols for enzyme stabilization via enzyme immobilization, such as sol-gel encapsulation, polymer encapsulation, and single enzyme nanoparticle formation. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and current, Enzyme Stabilization and Immobilization: Methods and Protocols is an essential handbook for all molecular biologists, biochemists, and biomedical and biochemical engineers.
- Table of contents (17 chapters)
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Introduction to the Field of Enzyme Immobilization and Stabilization
Pages 1-7
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Stabilization of Enzymes Through Encapsulation in Liposomes
Pages 9-18
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Micellar Enzymology for Thermal, pH, and Solvent Stability
Pages 19-24
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Lipase Activation and Stabilization in Room-Temperature Ionic Liquids
Pages 25-35
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Nanoporous Silica Glass for the Immobilization of Interactive Enzyme Systems
Pages 37-48
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Table of contents (17 chapters)
- Download Preface 1 PDF (31.5 KB)
- Download Sample pages 1 PDF (111 KB)
- Download Table of contents PDF (43.3 KB)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Enzyme Stabilization and Immobilization
- Book Subtitle
- Methods and Protocols
- Editors
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- Shelley D. Minteer
- Series Title
- Methods in Molecular Biology
- Series Volume
- 679
- Copyright
- 2011
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC
- eBook ISBN
- 978-1-60761-895-9
- DOI
- 10.1007/978-1-60761-895-9
- Hardcover ISBN
- 978-1-60761-894-2
- Softcover ISBN
- 978-1-4939-5792-7
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- X, 229
- Topics
