Methods in Molecular Biology

MicroRNAs and the Immune System

Methods and Protocols

Editors: Monticelli, Silvia (Ed.)

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  • Offers cutting edge methods to analyse microRNA expression, function and post-transcriptional modifications
  • Includes methods to isolate and manipulate cells of the immune system
  • Provides a description of bioinformatics databases on miRNAs and their targets, as well as instructions to navigate them
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eBook 117,69 €
price for Spain (gross)
  • ISBN 978-1-60761-811-9
  • Digitally watermarked, DRM-free
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  • Immediate eBook download after purchase and usable on all devices
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Hardcover 176,79 €
price for Spain (gross)
Softcover 150,79 €
price for Spain (gross)
About this book

In recent years, the critical role of microRNAs has been revealed within the biology of cells that constitute the immune system. In MicroRNAs and the Immune System: Methods and Protocols, expert researchers explore the latest techniques for studying miRNA expression, including the most up-to-date data on splinted ligation and qRT-PCR assays, as well as high-throughput profiling through cloning, deep sequencing, and microarrays. Chapters outline methods to study miRNA functions in various cell types from a single cell type level to entire model organisms, and present studies of miRNAs in the context of viruses and the immune response. Tools are also provided to help navigate bioinformatics databases on miRNAs and their targets. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Contemporary and innovative, MicroRNAs and the Immune System: Methods and Protocols is an essential handbook for immunologists, biochemists, and molecular biologists.

Reviews

From the reviews:

“This book is useful to scientists seeking to study the role of miRNAs in the immune system. … helpful to immunologists, biochemists, and molecular biologists interested in miRNA effects. The detailed protocols are invaluable to students and post-docs in particular. … The figures of the types of data one may obtain and the notes on troubleshooting are invaluable to both seasoned investigators and novice students.” (Elizabeth S. Raveche, Doody’s Review Service, February, 2011)

“Serves primarily as a recipe book, detailing all the latest methods one would need to further investigate the roles of microRNAs in this area of biology. It will appeal to both students who are new to the field … and molecular biologists who can keep it close as a handy reference material for planning and carrying out their experiments. … appealing to anyone from beginners to experts. … an attractive book for those interested in identifying and characterising particular miRNAs in the immune system.” (Daniel Peters, Immunology News, February, 2011)


Table of contents (21 chapters)

Table of contents (21 chapters)
  • A Rapid, Quantitative Assay for Direct Detection of MicroRNAs and Other Small RNAs Using Splinted Ligation

    Pages 3-17

    Chamnongpol, Sangpen (et al.)

  • Normalization of MicroRNA Quantitative RT-PCR Data in Reduced Scale Experimental Designs

    Pages 19-31

    Latham, Gary J.

  • MicroRNA Detection in Bone Marrow Cells by LNA-FISH

    Pages 33-45

    Debernardi, Silvana (et al.)

  • Measuring MicroRNA Expression in Size-Limited FACS-Sorted and Microdissected Samples

    Pages 47-63

    Hoefig, Kai P. (et al.)

  • MicroRNA Cloning from Cells of the Immune System

    Pages 67-77

    Wu, Haoquan (et al.)

Buy this book

eBook 117,69 €
price for Spain (gross)
  • ISBN 978-1-60761-811-9
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • Immediate eBook download after purchase and usable on all devices
  • Bulk discounts available
Hardcover 176,79 €
price for Spain (gross)
Softcover 150,79 €
price for Spain (gross)
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Bibliographic Information

Bibliographic Information
Book Title
MicroRNAs and the Immune System
Book Subtitle
Methods and Protocols
Editors
  • Silvia Monticelli
Series Title
Methods in Molecular Biology
Series Volume
667
Copyright
2010
Publisher
Humana Press
Copyright Holder
Springer Science + Business Media, LLC
eBook ISBN
978-1-60761-811-9
DOI
10.1007/978-1-60761-811-9
Hardcover ISBN
978-1-60761-810-2
Softcover ISBN
978-1-4939-6150-4
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIII, 330
Topics