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Stem Cells for Myocardial Regeneration

Methods and Protocols

  • Book
  • © 2010

Overview

Editors:
  1. Randall J. Lee

    1. , Division of Cardiology, University of California, San Francisco, San Francisco, USA

    View editor publications

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  • Provides comprehensive methods and reviews to help investigators assess the potential of stem cells for myocardial repair and regeneration
  • Presents guidelines for the reliable isolation of commonly assessed stem cells for myocardial repair
  • Includes cutting-edge methods to determine engraftment, efficacy, lectrical integration, and safety
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 660)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Isolation and Characterization of Cells for Myocardial Regeneration

    1. Front Matter

      Pages 7-7
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  3. Stem Cells for Myocardial Repair and Regeneration: Where Are We Today?

    1. Stem Cells for Myocardial Repair and Regeneration: Where Are We Today?

      • Randall J. Lee
      Pages 1-6

  4. Isolation and Characterization of Cells for Myocardial Regeneration

    1. Front Matter

      Pages 7-7
      Download chapter PDF

    2. Bone Marrow-Derived Endothelial Progenitor Cells: Isolation and Characterization for Myocardial Repair

      • Masaaki Ii
      Pages 9-27

    3. Umbilical Cord Blood Stem Cells for Myocardial Repair and Regeneration

      • Nicholas Greco, Mary J. Laughlin
      Pages 29-52

    4. Isolation of Resident Cardiac Progenitor Cells by Hoechst 33342 Staining

      • Otmar Pfister, Angelos Oikonomopoulos, Konstantina-Ioanna Sereti, Ronglih Liao
      Pages 53-63

    5. Mesenchymal Stem Cell Therapy for Cardiac Repair

      • Andrew J. Boyle, Ian K. McNiece, Joshua M. Hare
      Pages 65-84

    6. Methods for Human Embryonic Stem Cells Derived Cardiomyocytes Cultivation, Genetic Manipulation, and Transplantation

      • Gil Arbel, Oren Caspi, Irit Huber, Amira Gepstein, Michal Weiler-Sagie, Lior Gepstein
      Pages 85-95

  5. Animal Models for Assessing Stem Cells for Myocardial Regeneration

    1. Front Matter

      Pages 97-97
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    2. Experimental Cell Transplantation Therapy in Rat Myocardial Infarction Model Including Nude Rat Preparation

      • Wangde Dai, Robert A. Kloner
      Pages 99-109

    3. Large Animal Model of Heart Failure for Assessment of Stem Cells

      • Sharad Rastogi
      Pages 111-121

  6. Histological Assessment of Myocardial Regeneration

    1. Front Matter

      Pages 123-123
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    2. Histopathologic Assessment of Myocardial Regeneration

      • Naima Carter-Monroe, Elena Ladich, Renu Virmani, Frank D. Kolodgie
      Pages 125-148

    3. Assessment of Myocardial Angiogenesis and Vascularity in Small Animal Models

      • Matthew L. Springer
      Pages 149-167

  7. Measures of Cell Trafficking

    1. Front Matter

      Pages 169-169
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    2. Superparamagnetic Iron Oxide Labeling of Stem Cells for MRI Tracking and Delivery in Cardiovascular Disease

      • Dorota A. Kedziorek, Dara L. Kraitchman
      Pages 171-183

    3. Embryonic Stem Cell Biology: Insights from Molecular Imaging

      • Karim Sallam, Joseph C. Wu
      Pages 185-199

    4. Genetic Fate-Mapping for Studying Adult Cardiomyocyte Replenishment After Myocardial Injury

      • Sunny S.-K. Chan, Ying-Zhang Shueh, Nirma Bustamante, Shih-Jung Tsai, Hua-Lin Wu, Jyh-Hong Chen et al.
      Pages 201-211

  8. Electrophysiology

    1. Front Matter

      Pages 213-213
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Keywords

About this book

The feld of regenerative medicine is in its infancy state. Enthusiasm for the potential of organ regeneration lies with the potential pluripotency of stem cells to differentiate into various tissue types. This volume of Methods in Molecular Biology will focus on the use of stem cells for myocardial repair and regeneration. The emphasis of this issue will be to provide basic scientists, translational investigators, and cardiologists a means to evaluate the effcacy and safety of stem cells in a standardized fashion for myocardial regeneration. Many different cell types have been considered for myocardial repair. Adult card- myocytes are unable to survive even when transplanted into normal myocardium. The use of fetal or neonatal cardiomyocytes is not a feasible source of cells due to ethical concerns and donor availability. Therefore, the use of pluripotent stem cells has become the focus of a cell source for myocardial repair and regeneration. A variety of stem cell types have been suggested to participate in myocardial repair. This has led the investigators to search for the “optimal cell type for myocardial repair”. Reliable isolation of the cell source with the ability to expand the cell population is a prerequisite. In the frst section of this book, methods for isolation of commonly used stem cells being investigated for myocardial regeneration are presented. Once a stem cell source has been selected, the stem cell needs to be tested in an app- priate animal model before being translated into clinical practice.

Editors and Affiliations

  • , Division of Cardiology, University of California, San Francisco, San Francisco, USA

    Randall J. Lee

Bibliographic Information

  • Book Title: Stem Cells for Myocardial Regeneration

  • Book Subtitle: Methods and Protocols

  • Editors: Randall J. Lee

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-60761-705-1

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science + Business Media 2010

  • Hardcover ISBN: 978-1-60761-704-4Published: 09 August 2010

  • Softcover ISBN: 978-1-4939-6126-9Published: 23 August 2016

  • eBook ISBN: 978-1-60761-705-1Published: 03 August 2010

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 267

  • Topics: Cardiology, Cell Biology, Stem Cells, Gene Therapy

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