Methods in Molecular Biology

Phospho-Proteomics

Methods and Protocols

Editors: de Graauw, Marjo (Ed.)

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  • Comprehensive phospho-proteomics laboratory manual and research guide for both novice and experienced researchers
  • Provides step-by-step protocols written by leading researchers in the field that are fully adaptable to any specific phospho-proteome analysis task
  • Includes two detailed critical reviews on qualitative and quantitative mass-spectrometry-based phospho-proteomic analysis
  • Covers both basic and state-of-the-art practical procedures as well as bio-informatics approaches to explore the phospho-proteome
  • A compendium of cutting-edge protocols for the identification of phospho-proteins
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eBook 117,69 €
price for Spain (gross)
  • ISBN 978-1-60327-834-8
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  • Included format: EPUB, PDF
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  • Immediate eBook download after purchase
Hardcover 150,79 €
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Softcover 150,79 €
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About this book

Protein phosphorylation controls many basic cellular processes, such as cell growth, differentiation, migration, metabolism, and cell death, and its study can provide key insights into the signal transduction pathways that are activated in cells in response to different stimuli, such as growth factor stimulation or exposure to toxicants. In Phospho-Proteomics: Methods and Protocols, expert researchers contribute both well-established protocols and some of the newest strategies for the identification and evaluation of protein phosphorylation on Tyr, Ser, and Thr residues, including topics such as 2-dimensional gel electrophoresis and protein phosphorylation, enrichment of phospho-proteins and peptides, quantitative analysis of phosphorylation by labeling and MS analysis, and antibody and kinase arrays. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Phospho-Proteomics: Methods and Protocols is an ideal reference for both new and experienced scientists who wish to gain insight into the current developments in the field and to find inspiration to pursue its future.

Table of contents (23 chapters)

Table of contents (23 chapters)
  • A High-Resolution Two Dimensional Gel- and Pro-Q DPS-Based Proteomics Workflow for Phosphoprotein Identification and Quantitative Profiling

    Pages 3-19

    Agrawal, Ganesh K. (et al.)

  • Differential Phosphoprotein Labelling (DIPPL) Using 32P and 33P

    Pages 21-29

    Tolkovsky, Aviva M. (et al.)

  • Identification of Oxidative Stress-Induced Tyrosine Phosphorylated Proteins by Immunoprecipitation and Mass Spectrometry

    Pages 33-45

    Duran, Ma Carmen (et al.)

  • Enrichment and Characterization of Phosphopeptides by Immobilized Metal Affinity Chromatography (IMAC) and Mass Spectrometry

    Pages 47-56

    Thingholm, Tine E. (et al.)

  • The Use of Titanium Dioxide Micro-Columns to Selectively Isolate Phosphopeptides from Proteolytic Digests

    Pages 57-66

    Thingholm, Tine E. (et al.)

Buy this book

eBook 117,69 €
price for Spain (gross)
  • ISBN 978-1-60327-834-8
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 150,79 €
price for Spain (gross)
Softcover 150,79 €
price for Spain (gross)
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Bibliographic Information

Bibliographic Information
Book Title
Phospho-Proteomics
Book Subtitle
Methods and Protocols
Editors
  • Marjo de Graauw
Series Title
Methods in Molecular Biology
Series Volume
527
Copyright
2009
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-60327-834-8
DOI
10.1007/978-1-60327-834-8
Hardcover ISBN
978-1-60327-833-1
Softcover ISBN
978-1-61737-917-8
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 332
Number of Illustrations
69 b/w illustrations
Topics