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PCR Protocols

Current Methods and Applications

  • Book
  • © 1993

Overview

Editors:
  1. Bruce A. White

    1. University of Connecticut Health Center, Farmington

    View editor publications

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Part of the book series: Methods in Molecular Biology (MIMB, volume 15)

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Table of contents (35 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Polymerase Chain Reaction

    • Beverly C. Delidow, John P. Lynch, John J. Peluso, Bruce A. White
    Pages 1-29

  3. Selection of Primers for Polymerase Chain Reaction

    • Wojciech Rychlik
    Pages 31-40

  4. Direct Radioactive Labeling of Polymerase Chain Reaction Products

    • Tim McDaniel, Stephen J. Meltzer
    Pages 41-43

  5. Use of Arithmetic Polymerase Chain Reaction for Synthesis of Single-Stranded Probes for S1 Nuclease Assays

    • Puja Agarwal, Bruce A. White
    Pages 45-50

  6. Nonradioactive Labeling of Polymerase Chain Reaction Products

    • Udo Reischl, Rüdiger Rüger, Christoph Kessler
    Pages 51-62

  7. Quantitation and Purification of Polymerase Chain Reaction Products by High-Performance Liquid Chromatography

    • Elena D. Katz
    Pages 63-73

  8. Use of Polymerase Chain Reaction for Screening Transgenic Mice

    • Shizhong Chen, Glen A. Evans
    Pages 75-80

  9. Polymerase Chain Reaction Analysis of DNA from Paraffin-Embedded Tissue

    • Matthias Volkenandt, Adam P. Dicker, Renato Fanin, Debabrata Banerjee, Anthony Albino, Joseph R. Bertino
    Pages 81-88

  10. The Use of Polymerase Chain Reaction for Chromosome Assignment

    • Michael Jaye, Craig A. Dionne
    Pages 89-94

  11. Mapping MHC Class II Genes and Disease-Susceptibility

    • Stephen C. Bain, John A. Todd
    Pages 95-112

  12. The Use of the Polymerase Chain Reaction and the Detection of Amplified Products

    • Robert C. Allen, Bruce Budowle
    Pages 113-128

  13. Determination of Loss of Heterozygosity Using Polymerase Chain Reaction

    • Stephen J. Meltzer
    Pages 129-136

  14. Direct Sequencing of Polymerase Chain Reaction Products

    • Stephen J. Meltzer
    Pages 137-141

  15. Manual and Automated Direct Sequencing of Product Generated by the Polymerase Chain Reaction

    • Adam P. Dicker, Matthias Volkenandt, Joseph R. Bertino
    Pages 143-152

  16. Genomic Footprinting by Ligation Mediated Polymerase Chain Reaction

    • Gerd P. Pfeifer, Arthur D. Riggs
    Pages 153-168

  17. RNA Template-Specific Polymerase Chain Reaction (RS-PCR)

    • Alan R. Shuldiner, Riccardo Perfetti, Jesse Roth
    Pages 169-176

  18. Quantitative Measurement of Relative Gene Expression in Human Tumors

    • Tetsuro Horikoshi, Kathleen Danenberg, Matthias Volkenandt, Thomas Stadlbauer, Peter V. Danenberg
    Pages 177-188

  19. Identification of Alternatively Spliced mRNAs and Localization of 5' Ends by Polymerase Chain Reaction Amplification

    • Cheng-Ming Chiang, Louise T. Chow, Thomas R. Broker
    Pages 189-198

  20. Utilization of Polymerase Chain Reaction for Clonal Analysis of Gene Expression

    • Alice L. Witsell, Lawrence B. Schook
    Pages 199-204
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About this book

PCR has been successfully utilized in every facet of basic, cli- cal, and applied studies of the life sciences, and the impact that PCR has had on life science research is already staggering. C- comitant with the essentially universal use of PCR has been the creative and explosive development of a wide range of PCR-based techniques and applications. These increasingly numerous pro- cols have each had the general effect of facilitating and acceler- ing research. Because PCR technology is relatively easy and inexpensive, PCR applications are well within the reach of every research lab. In this sense, PCR has become the "equalizer" between "small" and "big" labs, since its use makes certain projects, especially those related to molecular cloning, now far more feasible for the small lab with a modest budget. This new volume on PCR Protocols does not attempt the impossible task of representing all PCR-based protocols. Rather, it presents a range of protocols, both analytical and preparative, that provide a solid base of knowledge on the use of PCR in many c- mon research problems. The first six chapters provide some basic information on how to get started. Chapters 7-19 represent primarily analytical uses of PCR, both for simple DNA and RNA detection, as well as for more complex analyses of nucleic acid (e. g. , DNA footprin ting, RNA splice site localization). The remaining chapters represent "synthetic," or preparative, uses of PCR.

Reviews

...affords many new insights and contributions of the first order...an excellent book of the highest interest.-Cellular and Molecular Biology

Editors and Affiliations

  • University of Connecticut Health Center, Farmington

    Bruce A. White

Bibliographic Information

  • Book Title: PCR Protocols

  • Book Subtitle: Current Methods and Applications

  • Editors: Bruce A. White

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/0896032442

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 1993

  • eBook ISBN: 978-1-59259-502-0Published: 02 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 392

  • Topics: Cell Biology

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