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Methods in Molecular Biology

Electron Microscopy Methods and Protocols

Editors: Hajibagheri, M. A. Nasser (Ed.)

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  • ISBN 978-1-59259-201-2
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About this book

In Electron Microscopy Methods and Protocols, well-practiced experts describe in detail the key electron microscopy techniques used for examining cells, tissue, biological macromolecules, molecular structure, and their interactions. With emphasis on cryotechniques for quantitative biological X-ray microanalysis, the book also includes those methods that use antibodies to locate proteins within cells and that prepare and analyze nucleic acids, proteins, and protein-nucleic acid complexes. Numerous immunogold labeling techniques for precise ultrastructural localization, distribution, and quantitation of macromolecules in cryo-fixed or chemically-fixed cells are described in sufficient detail to provide practical insight into their advantages and limitations.
Electron Microscopy Methods and Protocols offers both newcomers and established researchers wanting to expand their repertoire of cutting-edge electron microscopy techniques-each optimized for reproducibility and robust results-today's gold-standard laboratory manual.


Reviews

"Chapter after chapter, methods are described in detail following a step-by-step schedule giving sufficient details to lead the researcher to precise and reproducible work. . .One of the great features of this book is a series of notes concluding every chapter. These notes pool a lot of important recommendations. If I can give a piece of good advice, just for these notes, buy this book and read all this valuable information."-Biochemie

"This ring-bound volume provides the reader with a useful source of comprehensive and accurate protocols for electron microscopic specimen preparation with an emphasis on the subcellular level..."-Microbiology Today

Table of contents (15 chapters)

Table of contents (15 chapters)
  • General Preparation of Material and Staining of Sections

    Pages 1-11

    Davies, Heather A.

  • Negative Staining of Thinly Spread Biological Particulates

    Pages 13-30

    Robin Harris, J.

  • Preparation of Thin-Film Frozen-Hydrated/ Vitrified Biological Specimens for Cryoelectron Microscopy

    Pages 31-48

    Robin Harris, J. (et al.)

  • The Production of Cryosections Through Fixed and Cryoprotected Biological Material and Their Use in Immunocytochemistry

    Pages 49-76

    Webster, Paul

  • High-Pressure Freezing for Preservation of High Resolution Fine Structure and Antigenicity for Immunolabeling

    Pages 77-97

    McDonald, Kent

Buy this book

eBook 67,82 €
price for Spain (gross)
  • ISBN 978-1-59259-201-2
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
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Bibliographic Information

Bibliographic Information
Book Title
Electron Microscopy Methods and Protocols
Editors
  • M. A. Nasser Hajibagheri
Series Title
Methods in Molecular Biology
Series Volume
117
Copyright
1999
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59259-201-2
DOI
10.1385/1592592015
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 283
Topics