Electron Microscopy Methods and Protocols
Editors: Hajibagheri, M. A. Nasser (Ed.)
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- About this book
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In Electron Microscopy Methods and Protocols, well-practiced experts describe in detail the key electron microscopy techniques used for examining cells, tissue, biological macromolecules, molecular structure, and their interactions. With emphasis on cryotechniques for quantitative biological X-ray microanalysis, the book also includes those methods that use antibodies to locate proteins within cells and that prepare and analyze nucleic acids, proteins, and protein-nucleic acid complexes. Numerous immunogold labeling techniques for precise ultrastructural localization, distribution, and quantitation of macromolecules in cryo-fixed or chemically-fixed cells are described in sufficient detail to provide practical insight into their advantages and limitations.
Electron Microscopy Methods and Protocols offers both newcomers and established researchers wanting to expand their repertoire of cutting-edge electron microscopy techniques-each optimized for reproducibility and robust results-today's gold-standard laboratory manual.
- Reviews
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"Chapter after chapter, methods are described in detail following a step-by-step schedule giving sufficient details to lead the researcher to precise and reproducible work. . .One of the great features of this book is a series of notes concluding every chapter. These notes pool a lot of important recommendations. If I can give a piece of good advice, just for these notes, buy this book and read all this valuable information."-Biochemie
"This ring-bound volume provides the reader with a useful source of comprehensive and accurate protocols for electron microscopic specimen preparation with an emphasis on the subcellular level..."-Microbiology Today
- Table of contents (15 chapters)
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General Preparation of Material and Staining of Sections
Pages 1-11
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Negative Staining of Thinly Spread Biological Particulates
Pages 13-30
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Preparation of Thin-Film Frozen-Hydrated/ Vitrified Biological Specimens for Cryoelectron Microscopy
Pages 31-48
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The Production of Cryosections Through Fixed and Cryoprotected Biological Material and Their Use in Immunocytochemistry
Pages 49-76
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High-Pressure Freezing for Preservation of High Resolution Fine Structure and Antigenicity for Immunolabeling
Pages 77-97
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Table of contents (15 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Electron Microscopy Methods and Protocols
- Editors
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- M. A. Nasser Hajibagheri
- Series Title
- Methods in Molecular Biology
- Series Volume
- 117
- Copyright
- 1999
- Publisher
- Humana Press
- Copyright Holder
- Humana Press
- eBook ISBN
- 978-1-59259-201-2
- DOI
- 10.1385/1592592015
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XI, 283
- Topics
