- Offers over 200 key, readily reproducible protocols that ensure robust, successful results
- Includes many new techniques for the study of post-translational modifications
- Demonstrates the range of techniques available and their strengths and limitations
- Contains numerous time-saving techniques for even the most highly skilled researchers
Buy this book
- About this book
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Since the publication of the bestselling second edition of John Walker's widely acclaimed Protein Protocols Handbook, there have been continual methodological developments in the field of protein chemistry. This greatly enhanced third edition introduces 57 critically important new chapters, as well as significantly updating the previous edition's tried-and-true methods. Although the timely new chapters are spread throughout all of the book, the vital section on post-translational modifications has been expanded most to reflect the increasing importance of these modifications in the understanding of protein function.
Each readily reproducible method follows the highly praised format of the Methods in Molecular Biology™ series, offering a concise summary of its basic theory, a complete materials list, a step-by-step protocol for its successful execution, and extensive notes on avoiding pitfalls, or on modifying the method to function within your own experimental circumstances. The expert authors of each chapter have demonstrated a hands-on mastery of the methods described, fine-tuned here for optimal productivity.
Comprehensive, cutting-edge, and highly practical, The Protein Protocols Handbook, Third Edition is today's indispensable benchtop manual and guide, not only for all those new to the protein chemistry laboratory, but also for those established workers seeking to broaden their armametarium of techniques in the urgent search for rapid and robust results.
- Table of contents (49 chapters)
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Protein Determination by UV Absorption
Pages 3-6
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The Lowry Method for Protein Quantitation
Pages 7-10
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The Bicinchoninic Acid (BCA) Assay for Protein Quantitation
Pages 11-15
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The Bradford Method For Protein Quantitation
Pages 17-24
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Ultrafast Protein Determinations Using Microwave Enhancement
Pages 25-34
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Table of contents (49 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- The Protein Protocols Handbook
- Editors
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- John M. Walker
- Series Title
- Springer Protocols Handbooks
- Copyright
- 2009
- Publisher
- Humana Press
- Copyright Holder
- Humana Press
- eBook ISBN
- 978-1-59745-198-7
- DOI
- 10.1007/978-1-59745-198-7
- Hardcover ISBN
- 978-1-58829-880-5
- Softcover ISBN
- 978-1-60327-474-6
- Series ISSN
- 1949-2448
- Edition Number
- 3
- Number of Pages
- LXX, 1984
- Topics
