- Offers over 200 key, readily reproducible protocols that ensure robust, successful results
- Includes many new techniques for the study of post-translational modifications
- Demonstrates the range of techniques available and their strengths and limitations
- Contains numerous time-saving techniques for even the most highly skilled researchers
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- About this book
-
Since the publication of the bestselling second edition of John Walker's widely acclaimed Protein Protocols Handbook, there have been continual methodological developments in the field of protein chemistry. This greatly enhanced third edition introduces 57 critically important new chapters, as well as significantly updating the previous edition's tried-and-true methods. Although the timely new chapters are spread throughout all of the book, the vital section on post-translational modifications has been expanded most to reflect the increasing importance of these modifications in the understanding of protein function.
Each readily reproducible method follows the highly praised format of the Methods in Molecular Biology™ series, offering a concise summary of its basic theory, a complete materials list, a step-by-step protocol for its successful execution, and extensive notes on avoiding pitfalls, or on modifying the method to function within your own experimental circumstances. The expert authors of each chapter have demonstrated a hands-on mastery of the methods described, fine-tuned here for optimal productivity.
Comprehensive, cutting-edge, and highly practical, The Protein Protocols Handbook, Third Edition is today's indispensable benchtop manual and guide, not only for all those new to the protein chemistry laboratory, but also for those established workers seeking to broaden their armametarium of techniques in the urgent search for rapid and robust results.
- Table of contents (208 chapters)
-
-
Protein Determination by UV Absorption
Pages 3-6
Aitken, Alastair (et al.)
-
The Lowry Method for Protein Quantitation
Pages 7-10
Waterborg, Jakob H.
-
The Bicinchoninic Acid (BCA) Assay for Protein Quantitation
Pages 11-15
Walker, John M.
-
The Bradford Method For Protein Quantitation
Pages 17-24
Kruger, Nicholas J.
-
Ultrafast Protein Determinations Using Microwave Enhancement
Pages 25-34
Akins, Robert E. (et al.)
-
The Nitric Acid Method for Protein Estimation in Biological Samples
Pages 35-45
Boerner, Scott A. (et al.)
-
Quantitation of Tryptophan in Proteins
Pages 47-50
Aitken, Alastair (et al.)
-
Kinetic Silver Staining of Proteins
Pages 51-54
Root, Douglas D. (et al.)
-
Quantitation of Cellular Proteins by Flow Cytometry
Pages 55-61
Friedrich, Thomas D. (et al.)
-
Quantitation of Cellular Proteins by Laser Scanning Cytometry
Pages 63-69
Friedrich, Thomas D. (et al.)
-
Protein Solubility in Two-Dimensional Electrophoresis
Pages 73-84
Rabilloud, Thierry
-
Mouse and Human Tissue Sample Preparation for 2-D Electrophoresis
Pages 85-107
Zabel, Claus (et al.)
-
Plant Protein Sample Preparation for 2-DE
Pages 109-119
Carpentier, Sebastien Christian (et al.)
-
Preparation of Bacterial Samples for 2-D PAGE
Pages 121-130
Vandahl, Brian B. (et al.)
-
Preparation of Bodily Fluids for 2-D PAGE
Pages 131-138
Sullivan, Anthony G. (et al.)
-
Immunoaffinity Depletion of High Abundance Plasma and Serum Proteins
Pages 139-153
Echan, Lynn A. (et al.)
-
Preparation of Yeast Samples for 2-D PAGE
Pages 155-158
Norbeck, Joakim
-
Membrane Protein Preparation Using Aqueous Polymer Two-Phase Systems
Pages 159-164
Schindler, Jens (et al.)
-
Subcellular Fractionation of Small Sample Amounts
Pages 165-170
Nothwang, Hans Gerd (et al.)
-
Nondenaturing Polyacrylamide Gel Electrophoresis of Proteins
Pages 171-176
Walker, John M.
-
SDS Polyacrylamide Gel Electrophoresis of Proteins
Pages 177-185
Walker, John M.
-
Gradient SDS Polyacrylamide Gel Electrophoresis of Proteins
Pages 187-191
Walker, John M.
-
SDS-Polyacrylamide Gel Electrophoresis of Peptides
Pages 193-200
Judd, Ralph C.
-
Separation of Proteins by Blue Native Electrophoresis (BN-PAGE)
Pages 201-210
Dian, Elke A. (et al.)
-
Separation of Proteins by Gel Electrophoresis in the Tris-Taurine-HCl System
Pages 211-219
Luche, Sylvie (et al.)
-
Cetyltrimethylammonium Bromide Discontinuous Gel Electrophoresis of Proteins
Pages 221-238
Akins, Robert E. (et al.)
-
Acetic Acid—Urea Polyacrylamide Gel Electrophoresis of Basic Proteins
Pages 239-249
Waterborg, Jakob H.
-
Acid—Urea—Triton Polyacrylamide Gel Electrophoresis of Histones
Pages 251-262
Waterborg, Jakob H.
-
Isoelectric Focusing of Proteins in Ultra-Thin Polyacrylamide Gels
Pages 263-268
Walker, John M.
-
Serial Immobilized pH Gradient Isoelectric Focusing over pH 4–9
Pages 269-277
Poznanović, Slobodan (et al.)
-
Radiolabeling of Eukaryotic Cells and Subsequent Preparation for 2-Dimensional Electrophoresis
Pages 279-283
Bizios, Nick
-
Two-Dimensional Polyacrylamide Gel Electrophoresis of Proteins Using Carrier Ampholyte pH Gradients in the First Dimension
Pages 285-291
Gravel, Patricia
-
Vertical Agarose Electrophoresis and Electroblotting of High-Molecular-Weight Proteins
Pages 293-298
Greaser, Marion L. (et al.)
-
Two-Dimensional PAGE of High-Molecular-Mass Proteins
Pages 299-304
Oh-Ishi, Masamichi (et al.)
-
Casting Immobilized pH Gradients
Pages 305-322
Gianazza, Elisabetta
-
A Modified Nonequilibrium pH Gradient Electrophoresis (NEPHGE) Technique for Protein Separation
Pages 323-328
Carroll, James A.
-
Microchip Capillary Electrophoresis
Pages 329-359
Fogarty, Barbara A. (et al.)
-
Protein Separations in Microfluidic Chips
Pages 361-378
Chow, Andrea W. (et al.)
-
Difference Gel Electrophoresis (DIGE)
Pages 379-408
Friedman, David B. (et al.)
-
Comparing 2D Electrophoretic Gels Across Internet Databases: An Open Source Application
Pages 409-441
Lemkin, Peter F. (et al.)
-
Quantification of Radiolabeled Proteins in Polyacrylamide Gels
Pages 443-448
Springer, Wayne R.
-
Differential ProteoTope Radioactive Quantification of Protein Abundance Ratios
Pages 449-478
Wozny, Wojciech (et al.)
-
Quantification of Proteins on Polyacrylamide Gels
Pages 479-486
Smith, Bryan John
-
Using SDS-PAGE and Scanning Laser Densitometry to Measure Yield and Degradation of Proteins
Pages 487-496
Miles, Aaron P. (et al.)
-
Rapid and Sensitive Staining of Unfixed Proteins in Polyacrylamide Gels with Nile Red
Pages 497-504
Daban, Joan-Ramon (et al.)
-
Zn2+-Reverse Staining Technique
Pages 505-513
Fernandez-Patron, Carlos
-
Protein Staining with Calconcarboxylic Acid in Polyacrylamide Gels
Pages 515-520
Cong, Wei-Tao (et al.)
-
Detection of Proteins in Polyacrylamide Gels by Silver Staining
Pages 521-528
Dunn, Michael J.
-
Background-Free Protein Detection in Polyacrylamide Gels and on Electroblots Using Transition Metal Chelate Stains
Pages 529-545
Patton, Wayne F.
-
Detection of Proteins in Polyacrylamide Gels by Fluorescent Staining
Pages 547-554
Dunn, Michael J.
-
Detection of Glycoproteins in Gels and Blots
Pages 555-568
Packer, Nicolle H. (et al.)
-
Staining of Glycoproteins/Proteoglycans on SDS-Gels
Pages 569-574
Møller, Holger J. (et al.)
-
Detection of Proteins and Sialoglycoproteins in Polyacrylamide Gels Using Eosin Y Stain
Pages 575-578
Lin, Fan (et al.)
-
A Modified Pro-Q Diamond Staining Protocol for Phosphoprotein Detection in Polyacrylamide Gels
Pages 579-585
Agrawal, Ganesh Kumar (et al.)
-
Electroelution of Proteins from Polyacrylamide Gels
Pages 587-594
Jenö, Paul (et al.)
-
Autoradiography and Fluorography of Acrylamide Gels
Pages 595-603
Circolo, Antonella (et al.)
-
Proteolytic Activity Detection by Two-Dimensional Zymography
Pages 605-613
Wilkesman, Jeff
-
Protein Blotting by Electroblotting
Pages 617-619
Page, Mark (et al.)
-
Protein Blotting by the Semidry Method
Pages 621-629
Gravel, Patricia
-
Protein Blotting by the Capillary Method
Pages 631-633
Walker, John M.
-
Western Blotting of Basic Proteins Electrophoretically Resolved on Acid-Urea-Triton-Polyacrylamide Gels
Pages 635-639
Delcuve, Geneviève P. (et al.)
-
Immunoblotting of 2-DE Separated Proteins
Pages 641-662
Magi, Barbara (et al.)
-
High-Efficiency Blotting of High-Molecular Weight Proteins
Pages 663-671
Greaser, Marion L. (et al.)
-
Alkaline Phosphatase Labeling of IgG Antibody
Pages 673-675
Wisdom, G. Brian
-
β-Galactosidase Labeling of IgG Antibody
Pages 677-679
Wisdom, G. Brian
-
Horseradish Peroxidase Labeling of IgG Antibody
Pages 681-683
Wisdom, G. Brian
-
Digoxigenin Labeling of IgG Antibody
Pages 685-686
Wisdom, G. Brian
-
Conjugation of Fluorochromes to Antibodies
Pages 687-691
Mao, Su-Yau
-
Coupling of Antibodies with Biotin
Pages 693-703
Haugland, Rosaria P. (et al.)
-
Preparation of Avidin Conjugates
Pages 705-716
Haugland, Rosaria P. (et al.)
-
MDPF Staining of Proteins on Western Blots
Pages 717-721
Alba, F. Javier (et al.)
-
Copper Iodide Staining of Proteins and Its Silver Enhancement
Pages 723-728
Root, Douglas D. (et al.)
-
Detection of Proteins on Blots Using Direct Blue 71
Pages 729-735
Cong, Wei-Tao (et al.)
-
Detection of Proteins on Western Blots Using Colorimetric and Radiometric Visualization of Secondary Ligands
Pages 737-753
Kruger, Nicholas J.
-
Identification of Glycoproteins on Nitrocellulose Membranes Using Lectin Blotting
Pages 755-770
Gravel, Patricia
-
A Sensitive Method to Quantitatively Detect Total Protein on Membranes after Electrophoretic Transfer Using Avidin- or Streptavidin-Biotin
Pages 771-777
LaRochelle, William J.
-
Detection and Quantification of Proteins on Immunoblots using Enhanced Chemiluminescence
Pages 779-788
Young, Jennifer J.
-
Reutilization of Western Blots After Chemiluminescent or Autoradiographic Detection
Pages 789-806
Kaufmann, Scott H.
-
The Use of Quantum Dot Luminescent Probes for Western Blot Analysis
Pages 807-818
Makrides, Savvas C. (et al.)
-
The Use of Infrared Fluorescent Dyes in Quantitative Immunoblotting
Pages 819-826
Yang, Ching-Hui (et al.)
-
The Use of Infrared Fluorescent Dyes in Immunofluorescence Microscopy
Pages 827-833
Kasbek, Christopher (et al.)
-
Carboxymethylation of Cysteine Using lodoacetamide/lodoacetic Acid
Pages 837-839
Aitken, Alastair (et al.)
-
Performic Acid Oxidation
Pages 841-843
Aitken, Alastair (et al.)
-
Succinylation of Proteins
Pages 845-846
Aitken, Alastair (et al.)
-
Pyridylethylation of Cysteine Residues
Pages 847-850
Ward, Malcolm
-
Side Chain Selective Chemical Modifications of Proteins
Pages 851-854
Tawfik, Dan S.
-
Nitration of Tyrosines
Pages 855-858
Tawfik, Dan S.
-
Ethoxyformylation of Histidine
Pages 859-861
Tawfik, Dan S.
-
Modification of Arginine Side Chains with p-Hydroxyphenylglyoxal
Pages 863-864
Tawfik, Dan S.
-
Amidation of Carboxyl Groups
Pages 865-866
Tawfik, Dan S.
-
Amidination of Lysine Side Chains
Pages 867-869
Tawfik, Dan S.
-
Modification of Tryptophan with 2-Hydroxy-5-Nitrobenzylbromide
Pages 871-872
Tawfik, Dan S.
-
Modification of Sulfhydryl Groups with DTNB
Pages 873-874
Tawfik, Dan S.
-
Chemical Cleavage of Proteins at Methionyl-X Peptide Bonds
Pages 875-882
Smith, Bryan John
-
Chemical Cleavage of Proteins at Tryptophanyl-X Peptide Bonds
Pages 883-890
Smith, Bryan John
-
Chemical Cleavage of Proteins at Aspartyl-X Peptide Bonds
Pages 891-894
Smith, Bryan John
-
Chemical Cleavage of Proteins at Cysteinyl-X Peptide Bonds
Pages 895-898
Smith, Bryan John
-
Chemical Cleavage of Proteins at Asparaginyl-Glycyl Peptide Bonds
Pages 899-903
Smith, Bryan John
-
Enzymatic Digestion of Proteins in Solution and in SDS Polyacrylamide Gels
Pages 905-917
Stone, Kathryn L. (et al.)
-
On-PVDF Protein Digestions for N-terminal Sequencing and Peptide Mass Fingerprinting
Pages 919-925
Pham, Victoria (et al.)
-
Enzymatic Digestion of Membrane-Bound Proteins for Peptide Mapping and Internal Sequence Analysis
Pages 927-940
Fernandez, Joseph (et al.)
-
Reverse-Phase HPLC Separation of Enzymatic Digests of Proteins
Pages 941-950
Stone, Kathryn L. (et al.)
-
Peptide Mapping by Two-Dimensional Thin-Layer Electrophoresis-Thin-Layer Chromatography
Pages 951-962
Judd, Ralph C.
-
Peptide Mapping by Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis
Pages 963-968
Judd, Ralph C.
-
Peptide Mapping for Protein Characterization
Pages 969-988
Højrup, Peter
-
Production of Protein Hydrolysates Using Enzymes
Pages 989-993
Walker, John M. (et al.)
-
Amino Acid Analysis by Precolumn Derivatization with 1-Fluoro-2,4-Dinitrophenyl-5-I-Alanine Amide (Marfey’s Reagent)
Pages 995-1000
Kochhar, Sunil (et al.)
-
Amino Acid Analysis of Protein Hydrolysates Using Anion Exchange Chromatography and IPAD Detection
Pages 1001-1014
Jandik, Petr (et al.)
-
Validation of Amino Acid Analysis Methods
Pages 1015-1028
Reason, Andrew J.
-
Molecular Weight Estimation for Native Proteins Using High-Performance Size-Exclusion Chromatography
Pages 1029-1037
Irvine, G. Brent
-
Detection of Disulfide-Linked Peptides by HPLC
Pages 1039-1041
Aitken, Alastair (et al.)
-
Detection of Disulfide-Linked Peptides by Mass Spectrometry
Pages 1043-1046
Aitken, Alastair (et al.)
-
Diagonal Electrophoresis for Detecting Disulfide Bridges
Pages 1047-1051
Aitken, Alastair (et al.)
-
Estimation of Disulfide Bonds Using Ellman’s Reagent
Pages 1053-1055
Aitken, Alastair (et al.)
-
Quantitation of Cysteine Residues and Disulfide Bonds by Electrophoresis
Pages 1057-1062
Aitken, Alastair (et al.)
-
N-Terminal Sequencing of N-Terminally Modified Proteins
Pages 1063-1074
Kamp, Roza Maria (et al.)
-
Deblocking of Proteins Containing N-Terminal Pyroglutamic Acid
Pages 1075-1080
Mozdzanowski, Jacek
-
Detection and Characterization of Protein Mutations by Mass Spectrometry
Pages 1081-1094
Wada, Yoshinao
-
Peptide Sequencing by Nanoelectrospray Tandem Mass Spectrometry
Pages 1095-1115
Jensen, Ole Nýrregaard (et al.)
-
Protein Identification by Peptide Mass Fingerprinting using MALDI-TOF Mass Spectrometry
Pages 1117-1129
Webster, Judith (et al.)
-
Protein Ladder Sequencing
Pages 1131-1139
Wang, Rong (et al.)
-
Sequence Analysis with WinGene/WinPep
Pages 1141-1147
Hennig, Lars
-
HPLC and Mass Spectrometry of Integral Membrane Proteins
Pages 1149-1166
Whitelegge, Julian P.
-
Enrichment of Serum Peptides and Analysis by MALDI-TOF Mass Spectrometry
Pages 1167-1174
An, Yanming (et al.)
-
Computational Methods for Analysis of MALDI-TOF Spectra to Discover Peptide Serum Biomarkers
Pages 1175-1183
Ressom, Habtom W. (et al.)
-
Simple Tools for Complex N-Glycan Analysis
Pages 1187-1204
Fitchette, Anne-Catherine (et al.)
-
A Lectin-Binding Assay for the Rapid Characterization of the Glycosylation of Purified Glycoproteins
Pages 1205-1214
Goodarzi, Mohammad T. (et al.)
-
Chemical Methods of Analysis of Glycoproteins
Pages 1215-1217
Hounsell, Elizabeth F. (et al.)
-
Monosaccharide Analysis by HPAEC
Pages 1219-1221
Hounsell, Elizabeth F. (et al.)
-
Monosaccharide Analysis by Gas Chromatography (GC)
Pages 1223-1225
Hounsell, Elizabeth F. (et al.)
-
Determination of Monosaccharide Linkage and Substitution Patterns by GC-MS Methylation Analysis
Pages 1227-1229
Hounsell, Elizabeth F. (et al.)
-
Sialic Acid Analysis by HPAEC-PAD
Pages 1231-1232
Hounsell, Elizabeth F. (et al.)
-
Chemical Release of O-Linked Oligosaccharide Chains
Pages 1233-1234
Hounsell, Elizabeth F. (et al.)
-
O-Linked Oligosaccharide Profiling by HPLC
Pages 1235-1236
Hounsell, Elizabeth F. (et al.)
-
O-Linked Oligosaccharide Profiling by HPAEC-PAD
Pages 1237-1238
Hounsell, Elizabeth F. (et al.)
-
Release of N-Linked Oligosaccharide Chains by Hydrazinolysis
Pages 1239-1242
Mizuochi, Tsuguo (et al.)
-
Enzymatic Release of O-and N-Linked Oligosaccharide Chains
Pages 1243-1245
Hounsell, Elizabeth F. (et al.)
-
N-Linked Oligosaccharide Profiling by HPLC on Porous Graphitized Carbon (PGC)
Pages 1247-1248
Hounsell, Elizabeth F. (et al.)
-
N-Linked Oligosaccharide Profiling by HPAEC-PAD
Pages 1249-1251
Hounsell, Elizabeth F. (et al.)
-
HPAEC-PAD Analysis of Monosaccharides Released by Exoglycosidase Digestion Using the CarboPac MA1 Column
Pages 1253-1260
Weitzhandler, Michael (et al.)
-
Microassay Analyses of Protein Glycosylation
Pages 1261-1272
Wong, Nicky K. C. (et al.)
-
Polyacrylamide Gel Electrophoresis of Fluorophore-Labeled Carbohydrates from Glycoproteins
Pages 1273-1288
Brandley, Brian K. (et al.)
-
HPLC Analysis of Fluorescently Labeled Glycans
Pages 1289-1312
Merry, Tony (et al.)
-
Glycoprofiling Purified Glycoproteins Using Surface Plasmon Resonance
Pages 1313-1320
Fotinopoulou, Angeliki (et al.)
-
Sequencing Heparan Sulfate Saccharides
Pages 1321-1334
Turnbull, Jeremy E.
-
Analysis of Glycoprotein Heterogeneity by Capillary Electrophoresis and Mass Spectrometry
Pages 1335-1346
Hooker, Andrew D. (et al.)
-
Affinity Chromatography of Oligosaccharides and Glycopeptides with Immobilized Lectins
Pages 1347-1362
Yamamoto, Kazuo
-
In-Gel Enzymatic Release of N-Glycans
Pages 1363-1370
Harvey, David J.
-
Analysis of N-Linked Glycans by Mass Spectrometry
Pages 1371-1386
Harvey, David J.
-
MS Analysis of Protein Glycosylation
Pages 1387-1394
Takemori, Nobuaki (et al.)
-
Mapping protein N-Glycosylation by COFRADIC
Pages 1395-1402
Ghesquière, Bart (et al.)
-
Mass Spectrometric Analysis of O-Linked Glycans Released Directly from Glycoproteins in Gels Using β-Elimination
Pages 1403-1418
Taylor, Adrian M. (et al.)
-
Glycopeptide Analysis Using LC/MS and LC/MSn Site-Specific Glycosylation Analysis of a Glycoprotein
Pages 1419-1430
Itoh, Satsuki (et al.)
-
Identification of Vitamin K-Dependent Proteins Using a Gla-Specific Monoclonal Antibody
Pages 1431-1449
Hansson, Karin
-
The Identification of Protein S-Nitrosocysteine
Pages 1451-1465
Greco, Todd M. (et al.)
-
Detection of Nitrotyrosine-Containing Proteins
Pages 1467-1490
Zhan, Xianquan (et al.)
-
Mass Spectrometric Determination of Protein Ubiquitination
Pages 1491-1518
Parker, Carol E. (et al.)
-
Detection of Sumoylated Proteins
Pages 1519-1529
Park-Sarge, Ok-Kyong (et al.)
-
Efficient Enrichment of Intact Phosphorylated Proteins by Modified Immobilized Metal-Affinity Chromatography
Pages 1531-1545
Dubrovska, Anna
-
Analyzing Protein Phosphorylation
Pages 1547-1553
Colyer, John
-
Mass Spectrometric Analysis of Protein Phosphorylation
Pages 1555-1566
Gander, Stefan (et al.)
-
Protein Microarrays for Phosphorylation Studies
Pages 1567-1578
Kersten, Birgit (et al.)
-
Two-Dimensional Phosphopeptide Mapping
Pages 1579-1587
Nagahara, Hikaru (et al.)
-
Identification of Proteins Modified by Protein (D-Aspartyl/L-Isoaspartyl) Carboxyl Methyltransferase
Pages 1589-1599
Weber, Darin J. (et al.)
-
Analysis of Tyrosine-O-Sulfation
Pages 1601-1621
Bundgaard, Jens R. (et al.)
-
Analysis of Protein Palmitoylation by Metabolic Radiolabeling Methods
Pages 1623-1636
Pedone, Katherine H. (et al.)
-
Incorporation of Radiolabeled Prenyl Alcohols and Their Analogs into Mammalian Cell Proteins
Pages 1637-1655
Corsini, Alberto (et al.)
-
The Metabolic Labeling and Analysis of Isoprenylated Proteins
Pages 1657-1676
Andres, Douglas A. (et al.)
-
Antibody Production
Pages 1679-1686
Burns, Robert
-
Production of Antibodies Using Proteins in Gel Bands
Pages 1687-1691
Amero, Sally Ann (et al.)
-
Raising Highly Specific Polyclonal Antibodies Using Biocompatible Support-Bound Antigens
Pages 1693-1703
Diano, Monique (et al.)
-
Production of Antisera Using Peptide Conjugates
Pages 1705-1714
Adrian, Thomas E.
-
Small-Molecule—Protein Conjugation Procedures
Pages 1715-1726
Thompson, Stephen
-
The Chloramine T Method for Radiolabeling Protein
Pages 1727-1729
Bailey, Graham S.
-
The Lactoperoxidase Method for Radiolabeling Protein
Pages 1731-1732
Bailey, Graham S.
-
The Bolton and Hunter Method for Radiolabeling Protein
Pages 1733-1734
Bailey, Graham S.
-
Preparation of 125I-Labeled Peptides and Proteins with High Specific Activity Using IODO-GEN
Pages 1735-1742
Conlon, J. Michael
-
Purification and Assessment of Quality of Radioiodinated Protein
Pages 1743-1747
Bailey, Graham S.
-
Purification of IgG by Precipitation with Sodium Sulfate or Ammonium Sulfate
Pages 1749-1751
Page, Mark (et al.)
-
Purification of IgG Using Caprylic Acid
Pages 1753-1754
Page, Mark (et al.)
-
Purification of IgG Using DEAE-Sepharose Chromatography
Pages 1755-1756
Page, Mark (et al.)
-
Purification of IgG Using Ion-Exchange HPLC
Pages 1757-1758
Dolman, Carl (et al.)
-
Purification of IgG by Precipitation with Polyethylene Glycol (PEG)
Pages 1759-1760
Page, Mark (et al.)
-
Purification of IgG Using Protein A or Protein G
Pages 1761-1763
Page, Mark (et al.)
-
Analysis and Purification of IgG Using Size-Exclusion High Performance Liquid Chromatography (SE-HPLC)
Pages 1765-1767
Dolman, Carl (et al.)
-
Purification of IgG Using Affinity Chromatography on Antigen-Ligand Columns
Pages 1769-1772
Page, Mark (et al.)
-
Purification of IgG Using Thiophilic Chromatography
Pages 1773-1774
Page, Mark (et al.)
-
Analysis of IgG Fractions by Electrophoresis
Pages 1775-1777
Page, Mark (et al.)
-
Purification of Immunoglobulin Y (IgY) from Chicken Eggs
Pages 1779-1781
Bird, Christopher R. (et al.)
-
Affinity Purification of Immunoglobulins Using Protein A Mimetic (PAM)
Pages 1783-1797
Fassina, Giorgio (et al.)
-
Detection of Serological Cross-Reactions by Western Cross-Blotting
Pages 1799-1809
Hammerl, Peter (et al.)
-
Enzymatic Digestion of Monoclonal Antibodies
Pages 1811-1817
Andrew, Sarah M.
-
How to Make Bispecific Antibodies
Pages 1819-1826
French, Ruth R.
-
Antigen Measurement Using ELISA
Pages 1827-1833
Jordan, William
-
Enhanced Chemiluminescence Immunoassay
Pages 1835-1843
Stott, Richard A. W.
-
Immunoprecipitation and Blotting
Pages 1845-1858
Thompson, Stephen
-
Determination of Epitopes by Mass Spectrometry
Pages 1859-1872
Hager-Braun, Christine (et al.)
-
Immunogen Preparation and Immunization Procedures for Rats and Mice
Pages 1873-1875
Page, Mark (et al.)
-
Making Hybridomas
Pages 1877-1885
Burns, Robert
-
Growing Hybridomas
Pages 1887-1899
Entrican, Gary (et al.)
-
Mouse Hybridomas as an Entryway to Monoclonal Antibody Design and Production
Pages 1901-1909
Mechetner, Eugene
-
Potential Pitfalls in Monoclonal Antibody Development and Applications
Pages 1911-1927
Mechetner, Eugene
-
Recombinant Antibody Expression and Purification
Pages 1929-1943
Knappik, Achim (et al.)
-
Screening Hybridoma Culture Supernatants Using Solid-Phase Radiobinding Assay
Pages 1945-1946
Page, Mark (et al.)
-
Screening Hybridoma Culture Supernatants Using ELISA
Pages 1947-1948
Page, Mark (et al.)
-
Growth and Purification of Murine Monoclonal Antibodies
Pages 1949-1950
Page, Mark (et al.)
-
Affinity Purification Techniques for Monoclonal Antibodies
Pages 1951-1959
Schwarz, Alexander
-
A Rapid Method for Generating Large Numbers of High-Affinity Monoclonal Antibodies from a Single Mouse
Pages 1961-1974
Man, Nguyen Thi (et al.)
-
Table of contents (208 chapters)
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Bibliographic Information
- Bibliographic Information
-
- Book Title
- The Protein Protocols Handbook
- Editors
-
- John M. Walker
- Series Title
- Springer Protocols Handbooks
- Copyright
- 2009
- Publisher
- Humana Press
- Copyright Holder
- Humana Press
- eBook ISBN
- 978-1-59745-198-7
- DOI
- 10.1007/978-1-59745-198-7
- Hardcover ISBN
- 978-1-58829-880-5
- Softcover ISBN
- 978-1-60327-474-6
- Series ISSN
- 1949-2448
- Edition Number
- 3
- Number of Pages
- LXX, 1984
- Topics
