Skip to main content
SpringerLink
Log in
Book cover

Affinity Chromatography

Methods and Protocols

  • Book
  • © 2008

Overview

Editors:
  1. Michael Zachariou

    1. BioMarin Pharmaceutical Inc.

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Contains state-of-the-art, brand new protocols developed for this second edition
  • Provides step-by-step laboratory instructions for readily reproducible results

Part of the book series: Methods in Molecular Biology (MIMB, volume 421)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (21 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Affinity Chromatography

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Affinity Chromatography

      • Ana Cecília A. Roque, Christopher R. Lowe
      Pages 1-23

  3. Various Modes of Affinity Chromatography

    1. Front Matter

      Pages 23-23
      Download chapter PDF

    2. Immobilized Metal Ion Affinity Chromatography of Native Proteins

      • Adam Charlton, Michael Zachariou
      Pages 25-36

    3. Affinity Precipitation of Proteins Using Metal Chelates

      • Ashok Kumar, Igor Yu. Galaev, Bo Mattiasson
      Pages 37-52

    4. Immunoaffinity Chromatography

      • Stuart R. Gallant, Vish Koppaka, Nick Zecherle
      Pages 53-60

    5. Dye Ligand Chromatography

      • Stuart R. Gallant, Vish Koppaka, Nick Zecherle
      Pages 61-70

    6. Purification of Proteins Using Displacement Chromatography

      • Nihal Tugcu
      Pages 71-90

  4. Affinity Chromatography Using Purification Tags

    1. Front Matter

      Pages 91-91
      Download chapter PDF

    2. Rationally Designed Ligands for Use in Affinity Chromatography

      • Ana Cecília A. Roque, Christopher R. Lowe
      Pages 93-110

    3. Phage Display of Peptides in Ligand Selection for Use in Affinity Chromatography

      • Joanne L. Casey, Andrew M. Coley, Michael Foley
      Pages 111-124

    4. Preparation, Analysis and Use of an Affinity Adsorbent for the Purification of GST Fusion Protein

      • Gareth M. Forde
      Pages 125-136

    5. Immobilized Metal Ion Affinity Chromatography of Histidine-Tagged Fusion Proteins

      • Adam Charlton, Michael Zachariou
      Pages 137-150

    6. Methods for the Purification of HQ-Tagged Proteins

      • Becky Godat, Laurie Engel, Natalie A. Betz, Tonny M. Johnson
      Pages 151-168

    7. Amylose Affinity Chromatography of Maltose-Binding Protein

      • Leonard K Pattenden, Walter G Thomas
      Pages 169-190

    8. Methods for Detection of Protein–Proteinnl and Protein–DNA Interactions Using HaloTag ™

      • Marjeta Urh, Danette Hartzell, Jacqui Mendez, Dieter H. Klaubert, Keith Wood
      Pages 191-210

    9. Site-Specific Cleavage of Fusion Proteins

      • Adam Charlton
      Pages 211-228

    10. The Use of TAGZyme for the Efficient Removal of N-Terminal His-Tags

      • José Arnau, Conni Lauritzen, Gitte Ebert Petersen, John Pedersen
      Pages 229-243

  5. Various Applications of Affinity Chromatography

    1. Front Matter

      Pages 245-245
      Download chapter PDF
Back to top

Keywords

About this book

Forty years after the term “affinity chromatography” was introduced, this mode of chromatography remains a key tool in the armory of separation techniques that are available to separation and interaction scientists. Affinity chromat- raphy is favored because of its high selectivity, speed, and ease of use. The rapid and selective isolation of molecules using affinity chromatography has allowed a better understanding of biological processes, accelerated the identi- cation of target molecules, and spawned new process areas such as immobilized enzyme reactors. It has had ubiquitous application in most areas of science ranging from small molecule isolation to biopolymers such as DNA, proteins, polysaccharides, and even whole cells. The number of applications of affinity chromatography continues to expand at a rapid rate. For example, more than 60% of purification protocols include some sort of affinity chromatography step, while a database search of PubMed reveals more than 36,000 publications making use of the term “affinity chromatography,” more than 3000 of which refer to it in their title. The US patent office reports more than 16,000 ref- ences to the term “affinity chromatography”, while there are more than 270 references to the same term in the patent title. The aim of this edition of Methods in Molecular Biology, Affinity Chromatography: Methods and Protocols, Second Edition is to provide the beginner with the practical knowledge to develop affinity separations suitable for various applications relevant to the post-genomic era.

Reviews

From the reviews of the second edition:

"The book consists of three parts including 21 chapters that have been written by a large group of authors from many countries. … recommends this book as a manual for many specialists involved in purification of biomolecules in various fields of biochemistry, molecular biology, biotechnology, and bioorganic chemistry. The book can also be recommended as a handbook for university students and their teachers as well as for other schools preparing specialists in the above-mentioned fields." (G. Ya. Wiederschain, Biochemistry, November, 2008)

Editors and Affiliations

  • BioMarin Pharmaceutical Inc.

    Michael Zachariou

Bibliographic Information

  • Book Title: Affinity Chromatography

  • Book Subtitle: Methods and Protocols

  • Editors: Michael Zachariou

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-582-4

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2008

  • Hardcover ISBN: 978-1-58829-659-7Published: 30 November 2007

  • Softcover ISBN: 978-1-61737-701-3Published: 19 November 2010

  • eBook ISBN: 978-1-59745-582-4Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XII, 348

  • Number of Illustrations: 47 b/w illustrations

  • Topics: Cell Biology, Human Genetics, Biotechnology, Pharmacology/Toxicology

Publish with us

Policies and ethics

Back to top

Search

Navigation