Methods in Molecular Biology

Oligonucleotide Synthesis

Methods and Applications

Editors: Herdewijn, Piet (Ed.)

Free Preview

Buy this book

eBook 130,89 €
price for Spain (gross)
  • ISBN 978-1-59259-823-6
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 166,39 €
price for Spain (gross)
  • ISBN 978-1-58829-233-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
Softcover 166,39 €
price for Spain (gross)
  • ISBN 978-1-61737-441-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
About this book

Nucleic acids chemistry has progressed rapidly in the last 10 years with the introduction of many new techniques and the development of many novel therapeutic entities. In Oligonucleotide Synthesis: Methods and Applications, laboratory experts describe in step-by-step detail the powerful new techniques they have developed for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. Among the protocol highlights are the synthesis of phosphorothioates via new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, triple helix formation, and a novel two-step process that yields a high purity DNA that is less costly. Novel applications include the use of molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules to DNA, nucleic acid libraries, optimization of RNA synthesis for siRNA applications, and the use of small interference RNA (siRNA) as an inhibitor of gene expression. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and highly practical, Oligonucleotide Synthesis: Methods and Applications offers today's investigators not only insight into important new biotechnologies, but also a full range of the detailed protocols needed to work successfully in DNA synthesis and its applications today.

Table of contents (25 chapters)

Table of contents (25 chapters)

Buy this book

eBook 130,89 €
price for Spain (gross)
  • ISBN 978-1-59259-823-6
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 166,39 €
price for Spain (gross)
  • ISBN 978-1-58829-233-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
Softcover 166,39 €
price for Spain (gross)
  • ISBN 978-1-61737-441-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
Oligonucleotide Synthesis
Book Subtitle
Methods and Applications
Editors
  • Piet Herdewijn
Series Title
Methods in Molecular Biology
Series Volume
288
Copyright
2005
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59259-823-6
DOI
10.1385/1592598234
Hardcover ISBN
978-1-58829-233-9
Softcover ISBN
978-1-61737-441-8
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XVI, 436
Number of Illustrations
142 b/w illustrations
Topics