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Methods in Molecular Biology

Membrane Protein Protocols

Expression, Purification, and Characterization

Editors: Selinsky, Barry S. (Ed.)

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eBook 118,99 €
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  • ISBN 978-1-59259-400-9
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Hardcover 176,79 €
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  • ISBN 978-1-58829-124-0
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Softcover 155,99 €
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  • ISBN 978-1-61737-376-3
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About this book

Recent developments in molecular biology and biochemistry have made it possible to determine the three-dimensional structure of membrane proteins and so come to a full understanding of their function. In Membrane Protein Protocols: Expression, Purification, and Characterization, researchers at major universities and research centers around the world describe in detail the key techniques that have proven successful in the study of receptors and transport proteins. The book provides examples of how different membrane proteins can be overexpressed in both prokaryotic and eukaryotic expression systems, how natural and overexpressed proteins can be solubilized from their host membranes, and how the solubilized protein can be purified in active form. Each protocol contains step-by-step instructions to ensure success, troubleshooting advice, lists of reagents, and tips on avoiding pitfalls.
Timely and highly practical, Membrane Protein Protocols: Expression, Purification, and Characterization offers investigators proven methods to determine the three-dimensional structure of membrane receptors and transport proteins, as well as proven practical help in the discovery of new clinical targets for the design of novel pharmaceutical agents useful in treating cancer, bacterial and viral infection, and many metabolic diseases.

Reviews

"Investigators working with membrane proteins will want to acquire this book. This is a useful compilation of protocols. . .there is much of utility in this book"-Doody's Health Sciences Book Review Journal

"...a useful reference work for researchers investigating the structure and function of membrane proteins." -- Immunology News

"...provides good food for thought for new and advanced membrane protein researchers." - ChemBioChem

"...highly recommendable to people working in proteomics..." - Proteomics Journal

Table of contents (25 chapters)

Table of contents (25 chapters)

Buy this book

eBook 118,99 €
price for Spain (gross)
  • ISBN 978-1-59259-400-9
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 176,79 €
price for Spain (gross)
  • ISBN 978-1-58829-124-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
Softcover 155,99 €
price for Spain (gross)
  • ISBN 978-1-61737-376-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
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Bibliographic Information

Bibliographic Information
Book Title
Membrane Protein Protocols
Book Subtitle
Expression, Purification, and Characterization
Editors
  • Barry S. Selinsky
Series Title
Methods in Molecular Biology
Series Volume
228
Copyright
2003
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59259-400-9
DOI
10.1385/159259400X
Hardcover ISBN
978-1-58829-124-0
Softcover ISBN
978-1-61737-376-3
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XXII, 334
Topics