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Methods in Molecular Biology

Macrophage Migration Inhibitory Factor

Methods and Protocols

Editors: Harris, James, Morand, Eric F. (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook 117,69 €
price for Spain (gross)
  • ISBN 978-1-4939-9936-1
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Hardcover 145,59 €
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  • ISBN 978-1-4939-9935-4
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About this book

This volume looks at--and discusses the techniques of--a range of areas in biology in which macrophage migration inhibitory factor (MIF) is studied. Topics covered in this book include methods to quantify MIF in cell supernatants and human samples, using ELISA and western blot; detection and analysis of intracellular MIF using flow cytometry; genotyping of human MIF polymorphisms, detection of MIF expression in a marine invertebrate using rapid amplification of cDNA RACE; studying the MIF receptor CD74 in tissues using immunohistochemistry; and studying MIF in models of disease such as asthma, Plasmodium infection, and gout. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Comprehensive and practical, Macrophage Migration Inhibitory Factor: Methods and Protocols is a valuable resource that will help researchers gain a new understanding of MIF biology in health and disease. 


Table of contents (22 chapters)

Table of contents (22 chapters)

Buy this book

eBook 117,69 €
price for Spain (gross)
  • ISBN 978-1-4939-9936-1
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 145,59 €
price for Spain (gross)
  • ISBN 978-1-4939-9935-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
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Bibliographic Information

Bibliographic Information
Book Title
Macrophage Migration Inhibitory Factor
Book Subtitle
Methods and Protocols
Editors
  • James Harris
  • Eric F. Morand
Series Title
Methods in Molecular Biology
Series Volume
2080
Copyright
2020
Publisher
Springer US
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9936-1
DOI
10.1007/978-1-4939-9936-1
Hardcover ISBN
978-1-4939-9935-4
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 265
Number of Illustrations
9 b/w illustrations, 34 illustrations in colour
Topics