- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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- About this book
-
This volume looks at key methodologies that are commonly used across antibody drug conjugates (ADCs) programs. The chapters in this book cover topics such as conjugations to endogenous cysteine residues; click chemistry conjugations; antibody conjugations via glycosyl remodeling; analysis of ADCs by native mass spectrometry; characterization of ADCs by capillary electrophoresis; LC/MS methods for studying lysosomal ADC catabolism; and determination of ADC concentration by ligand-binding assays. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Antibody-Drug Conjugates: Methods and Protocols is a valuable resource that aims to lower the “activation barrier” when undertaking a new discipline, and provides a “toolbox” for the next generation of ADC scientists.
- Table of contents (26 chapters)
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An Overview of the Current ADC Discovery Landscape
Pages 1-22
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Pushing the Envelope: Advancement of ADCs Outside of Oncology
Pages 23-36
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Conjugations to Endogenous Cysteine Residues
Pages 37-49
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Site-Specific Conjugation to Cys-Engineered THIOMAB™ Antibodies
Pages 51-69
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Transglutaminase-Mediated Conjugations
Pages 71-82
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Table of contents (26 chapters)
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Bibliographic Information
- Bibliographic Information
-
- Book Title
- Antibody-Drug Conjugates
- Book Subtitle
- Methods and Protocols
- Editors
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- L. Nathan Tumey
- Series Title
- Methods in Molecular Biology
- Series Volume
- 2078
- Copyright
- 2020
- Publisher
- Springer US
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-4939-9929-3
- DOI
- 10.1007/978-1-4939-9929-3
- Hardcover ISBN
- 978-1-4939-9928-6
- Softcover ISBN
- 978-1-4939-9931-6
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XI, 373
- Number of Illustrations
- 29 b/w illustrations, 109 illustrations in colour
- Topics
