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Protein Nanotechnology

Protocols, Instrumentation, and Applications

  • Book
  • © 2020

Overview

Editors:
  1. Juliet A. Gerrard

    1. School of Biological Sciences, The MacDiarmid Institute for Advanced Materials and Nanotechnology, School of Chemical Sciences, University of Auckland, Auckland, New Zealand

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  2. Laura J. Domigan

    1. School of Biological Sciences, Department of Chemical and Materials Engineering, The MacDiarmid Institute for Advanced Materials and Nanotechnology, University of Auckland, Auckland, New Zealand

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2073)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Introduction to Protein Nanotechnology

    • Laura J. Domigan, Juliet A. Gerrard
    Pages 1-13

  3. Design and Production of Protein Building Blocks for Nanotechnology

    1. Front Matter

      Pages 15-15
      Download chapter PDF

    2. Protein Expression in the Baculovirus-Insect Cell Expression System

      • Adetayo A. Adeniyi, Linda Hwee-Lin Lua
      Pages 17-37

    3. Peroxiredoxin Proteins as Building Blocks for Nanotechnology

      • Frankie Conroy, N. Amy Yewdall
      Pages 39-54

    4. Formation of Amphipathic Amyloid Monolayers from Fungal Hydrophobin Proteins

      • Sarah R. Ball, Chi L. L. Pham, Victor Lo, Vanessa K. Morris, Ann H. Kwan, Margaret Sunde
      Pages 55-72

    5. ATP Synthase: Expression, Purification, and Function

      • Meghna Sobti, Robert Ishmukhametov, Alastair G. Stewart
      Pages 73-84

    6. Molecular Superglues: Discovery and Engineering Orthogonalization

      • Paul G. Young, Christopher J. Squire
      Pages 85-99

    7. Production of Multicomponent Protein Templates for the Positioning and Stabilization of Enzymes

      • Samuel Lim, Douglas S. Clark, Dominic J. Glover
      Pages 101-115

  4. Towards Protein Nano-Devices

    1. Front Matter

      Pages 117-117
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    2. Adding Function to Protein Scaffolds

      • Kyle Webster, Luigi Sasso, Laura J. Domigan
      Pages 119-147

    3. Virus-Derived Nanoparticles

      • Noor H. Dashti, Frank Sainsbury
      Pages 149-162

    4. Strategies for Increasing Protein Stability

      • Peter G. Chandler, Sebastian S. Broendum, Blake T. Riley, Matthew A. Spence, Colin J. Jackson, Sheena McGowan et al.
      Pages 163-181

    5. Generation of High-Affinity Molecularly Imprinted Nanoparticles for Protein Recognition via a Solid-Phase Synthesis Protocol

      • Francesco Canfarotta, Sergey A. Piletsky, Nicholas W. Turner
      Pages 183-194

    6. Nanotechnology with S-layer Proteins

      • Bernhard Schuster, Uwe B. Sleytr
      Pages 195-218

  5. Meaningful Measurements of Protein Nanocomponents

    1. Front Matter

      Pages 219-219
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    2. Preparation of Proteins and Macromolecular Assemblies for Cryo-electron Microscopy

      • Lou Brillault, Michael J. Landsberg
      Pages 221-246

    3. Atomic Force Microscopy of Proteins

      • Yiran An, Sesha Sarathchandra Manuguri, Jenny Malmström
      Pages 247-285

    4. Native Protein Mass Spectrometry

      • Timothy M. Allison, Mark T. Agasid
      Pages 287-299

    5. Nanoparticle Tracking Analysis of β-Casein Nanocarriers

      • Hikmat Masri, Jared K. Raynes
      Pages 301-309
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Keywords

About this book

This third edition volume expands on the previous editions with updated approaches and techniques used to study protein nanotechnology and the future of nanomaterial compositions. This book is organized into Three Parts: Part One looks at recombinant protein expression in insect cells, and methods to produce molecular motors, molecular superglues, and protein templates; Part Two explores functionalization strategies and ways to incorporate functional protein components into nanodevices; Part Three discusses various instrumental techniques used to study protein nanostructures. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 
Cutting-edge and thorough, Protein Nanotechnology: Protocols, Instrumentation,and Applications, Third Edition is a valuable resource for any researchers looking to expand their knowledge in this evolving field.
 


Editors and Affiliations

  • School of Biological Sciences, The MacDiarmid Institute for Advanced Materials and Nanotechnology, School of Chemical Sciences, University of Auckland, Auckland, New Zealand

    Juliet A. Gerrard

  • School of Biological Sciences, Department of Chemical and Materials Engineering, The MacDiarmid Institute for Advanced Materials and Nanotechnology, University of Auckland, Auckland, New Zealand

    Laura J. Domigan

Bibliographic Information

  • Book Title: Protein Nanotechnology

  • Book Subtitle: Protocols, Instrumentation, and Applications

  • Editors: Juliet A. Gerrard, Laura J. Domigan

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9869-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-4939-9868-5Published: 15 October 2019

  • Softcover ISBN: 978-1-4939-9871-5Published: 15 October 2020

  • eBook ISBN: 978-1-4939-9869-2Published: 14 October 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XIII, 331

  • Number of Illustrations: 20 b/w illustrations, 64 illustrations in colour

  • Topics: Protein Science, Biotechnology, Nanotechnology

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