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Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols

Cutting-Edge Technologies and Advancements

  • Book
  • © 2020

Overview

Editors:
  1. Yinduo Ji

    1. Department of Veterinary Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, Saint Paul, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2069)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Clinical, Epidemiologic, and Laboratory Aspects of Methicillin-Resistant Staphylococcus aureus Infections

    • Elizabeth L. Palavecino
    Pages 1-28

  3. Rapid Methods for Detection of MRSA in Clinical Specimens

    • Elizabeth L. Palavecino
    Pages 29-45

  4. Immunofluorescence Microscopy for the Detection of Surface Antigens in Methicillin Resistant Staphylococcus aureus (MRSA)

    • Yekaterina Timofeyeva, Ingrid L. Scully, Annaliesa S. Anderson
    Pages 47-58

  5. Staphylococcal Cassette Chromosome mec (SCCmec) Analysis of MRSA

    • Tetsuo Yamaguchi, Daisuke Ono, Ayami Sato
    Pages 59-78

  6. Pulsed-Field Gel Electrophoresis Typing of Staphylococcus aureus Strains

    • Yiping He, Sue Reed
    Pages 79-88

  7. Spa Typing of Staphylococcus aureus Isolates

    • Xin Wang
    Pages 89-94

  8. Multilocus Sequence Typing of Staphylococcus aureus

    • Ying Liu, Yinduo Ji
    Pages 95-102

  9. Genetic Manipulations of Staphylococcal Chromosomal DNA

    • Crystal M. Austin, Jeffrey L. Bose
    Pages 103-111

  10. Genetic Manipulation of MRSA Using CRISPR/Cas9 Technology

    • Weizhong Chen, Quanjiang Ji
    Pages 113-124

  11. Identification of Target Genes Mediated by Two-Component Regulators of Staphylococcus aureus Using RNA-seq Technology

    • Ting Lei, Junshu Yang, Aaron Becker, Yinduo Ji
    Pages 125-138

  12. Application of Two-Dimensional Difference Gel Electrophoresis in Identification of Factors Responsible for Virulence of Staphylococcus aureus

    • Emilia Bonar, Kinga Chlebicka, Grzegorz Dubin, Benedykt Wladyka
    Pages 139-154

  13. Identification of Virulence Determinants During Host-Pathogen Interaction Using Tn-Seq Technology

    • Christopher T. Peek, Carolyn B. Ibberson, James E. Cassat
    Pages 155-175

  14. Metabolomic Profiling of Staphylococcus aureus

    • Ting Lei, Qingqing Mao, Chi Chen, Yinduo Ji
    Pages 177-186

  15. Determining Impact of Growth Phases on Capacity of Staphylococcus aureus to Adhere to and Invade Host Cells

    • Nansea Ji, Junshu Yang, Yinduo Ji
    Pages 187-195

  16. Preclinical Models and Methodologies for Monitoring Staphylococcus aureus Infections Using Noninvasive Optical Imaging

    • Nathan K. Archer, Yu Wang, Roger V. Ortines, Haiyun Liu, Sabrina J. Nolan, Qi Liu et al.
    Pages 197-228

  17. Methicillin-Resistant Staphylococcus aureus Infection and Treatment Options

    • Sarah J. Chalmers, Mark E. Wylam
    Pages 229-251

  18. Animal Models for Drug Development for MRSA

    • Andrea Marra
    Pages 253-266

  19. Back Matter

    Pages 267-268
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Keywords

About this book

This third edition volume expands on the previous editions with an update on the latest techniques used for the detection, genotyping, and investigating pathogenesis of Staphylococcus aureus in vitro and in vivo. The methods covered in this book mostly focus on routine clinical diagnosis, surveillance, research, and practice for treatment of patients infected by multi-drug resistant S. aureus. The book also covers the epidemiology of MRSA, molecular typing approaches, clinical treatment of MRSA infections, and animal models of drug discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Informative and cutting-edge, Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols: Cutting-Edge Technologies and Advancements, Third Edition is a valuable resource for researchers looking to set up new methods to study S. aureus, and will also be very useful for technicians and scientists working on other bacterial pathogens. 


Editors and Affiliations

  • Department of Veterinary Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, Saint Paul, USA

    Yinduo Ji

Bibliographic Information

  • Book Title: Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols

  • Book Subtitle: Cutting-Edge Technologies and Advancements

  • Editors: Yinduo Ji

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9849-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-4939-9848-7Published: 16 September 2019

  • Softcover ISBN: 978-1-4939-9851-7Published: 16 September 2020

  • eBook ISBN: 978-1-4939-9849-4Published: 15 September 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XI, 268

  • Number of Illustrations: 8 b/w illustrations, 27 illustrations in colour

  • Topics: Bacteriology, Infectious Diseases

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