- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Buy this book
- About this book
-
This volume explores the latest techniques and workflow for the analysis of single cells metabolism. The chapters in this book cover topics such as the development of mass spectrometry-based single cell approaches, Pico-ESI-MS for single-cell metabolomics analysis; laser capture microdissection; ambient single cell metabolite profile (DESI and LAESI); and MALDI-MS methodology, quantum dots for quantitative cytology to study metabolic heterogeneity of single cells. Written in the highly successful Methods in Molecular Biology series format, the chapters consist of introductions to the topic, lists of the necessary materials and reagents, step-by-step guidelines, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Single Cell Metabolism: Methods and Protocols is a valuable resource for any researcher and scientist interested in learning more about this field.
- Table of contents (16 chapters)
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Single-Cell Metabolomics by Mass Spectrometry
Pages 1-8
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Identification of Metabolites in Single Cells by Ion Mobility Separation and Mass Spectrometry
Pages 9-18
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Analysis of Lipids in Single Cells and Organelles Using Nanomanipulation-Coupled Mass Spectrometry
Pages 19-30
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Development of Pico-ESI-MS for Single-Cell Metabolomics Analysis
Pages 31-59
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Single-Probe Mass Spectrometry Analysis of Metabolites in Single Cells
Pages 61-71
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Table of contents (16 chapters)
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Bibliographic Information
- Bibliographic Information
-
- Book Title
- Single Cell Metabolism
- Book Subtitle
- Methods and Protocols
- Editors
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- Bindesh Shrestha
- Series Title
- Methods in Molecular Biology
- Series Volume
- 2064
- Copyright
- 2020
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-4939-9831-9
- DOI
- 10.1007/978-1-4939-9831-9
- Hardcover ISBN
- 978-1-4939-9829-6
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XI, 226
- Number of Illustrations
- 10 b/w illustrations, 64 illustrations in colour
- Topics
*immediately available upon purchase as print book shipments may be delayed due to the COVID-19 crisis. ebook access is temporary and does not include ownership of the ebook. Only valid for books with an ebook version. Springer Reference Works are not included.
