Trinucleotide Repeats

1. Front Matter

   Pages i-xi

   PDF

2. Experimenting with Trinucleotide Repeats: Facts and Technical Issues

   • Guy-Franck Richard

   Pages 1-10

3. Fast Assays to Detect Interruptions in CTG.CAG Repeat Expansions

   • Stéphanie Tomé, Geneviève Gourdon

   Pages 11-23

4. Tracking Expansions of Stable and Threshold Length Trinucleotide Repeat Tracts In Vivo and In Vitro Using Saccharomyces cerevisiae

   • Gregory M. Williams, Athena K. Petrides, Lata Balakrishnan, Jennifer A. Surtees

   Pages 25-68

5. Quantifying Replication Fork Progression at CTG Repeats by 2D Gel Electrophoresis

   • David Viterbo, Guy-Franck Richard

   Pages 69-81

6. Genetic Assays to Study Repeat Fragility in Saccharomyces cerevisiae

   • Erica J. Polleys, Catherine H. Freudenreich

   Pages 83-101

7. Genetic Screens to Study GAA/TTC and Inverted Repeat Instability in Saccharomyces cerevisiae

   • Wenying Guo, Kirill S. Lobachev

   Pages 103-112

8. Monitoring Double-Strand Break Repair of Trinucleotide Repeats Using a Yeast Fluorescent Reporter Assay

   • Lucie Poggi, Bruno Dumas, Guy-Franck Richard

   Pages 113-120

9. Analysis of Trinucleotide Repeat Stability by Integration at a Chromosomal Ectopic Site

   • Rujuta Yashodhan Gadgil, S. Dean Rider Jr., Todd Lewis, Joanna Barthelemy, Michael Leffak

   Pages 121-136

10. Experimental System to Study Instability of (CGG)_n Repeats in Cultured Mammalian Cells

    • Artem V. Kononenko, Thomas Ebersole, Sergei M. Mirkin

    Pages 137-150

11. Assessing Triplet Repeat Expansions in Human SVG-A Cell Culture

    • Gregory M. Williams, Robert S. Lahue

    Pages 151-172

12. Isolation and Analysis of the CGG-Repeat Size in Male and Female Gametes from a Fragile X Mouse Model

    • Xiaonan Zhao, Huiyan Lu, Pradeep K. Dagur, Karen Usdin

    Pages 173-186

13. In Vitro Synthesis and RNA Structure Probing of CUG Triplet Repeat RNA

    • Remco T. P. van Cruchten, Derick G. Wansink

    Pages 187-202

14. FISH Protocol for Myotonic Dystrophy Type 1 Cells

    • Arnaud F. Klein, Ludovic Arandel, Joelle Marie, Denis Furling

    Pages 203-215

15. Real Time Videomicroscopy and Semiautomated Analysis of Brain Cell Culture Models of Trinucleotide Repeat Expansion Diseases

    • Sandra O. Braz, Diana M. Dinca, Geneviève Gourdon, Mário Gomes-Pereira

    Pages 217-240

16. Primary Cultures of Pure Embryonic Dorsal Root Ganglia Sensory Neurons as a New Cellular Model for Friedreich’s Ataxia

    • Olivier Griso, Hélène Puccio

    Pages 241-253

17. GFP Reporters to Monitor Instability and Expression of Expanded CAG/CTG Repeats

    • Cinzia Cinesi, Bin Yang, Vincent Dion

    Pages 255-268

18. Gene Therapy for Huntington’s Disease Using Targeted Endonucleases

    • Magdalena Dabrowska, Marta Olejniczak

    Pages 269-284

19. Back Matter

    Pages 285-286

    PDF

This detailed book contains techniques to explore the unusual properties of the peculiar microsatellites known as trinucleotide repeats.  Beginning with technical challenges raised by secondary structure-forming repeats and their propensity to contract and expand over time, the book continues with genetic screens in Saccharomyces cerevisiae, experimental systems to study trinucleotide repeat instability in human cells, dedicated experimental systems in appropriate cell types, as well as gene therapy approaches using the CRISPR-Cas family of endonucleases.  Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Trinucleotide Repeats: Methods and Protocols serves as avaluable aid to experts and newcomers alike who seek to investigate this fascinating and ever-expanding field of study.

• Genetic screens
• Pathologies
• Saccharomyces cerevisiae
• Human cells
• Experimental systems
• Gene therapy
• CRISPR-Cas
• Human disorders
• Genetic mutations

• Department Genomes & Genetics, Institut Pasteur, Paris, France

  Guy-Franck Richard

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