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Springer Protocols Handbooks

Fundamental Approaches to Screen Abnormalities in Drosophila

Editors: Mishra, Monalisa (Ed.)

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  • Explains in detail a range of methods used to test altered behavioral phenotypes in various developmental stages of Drosophila
  • Discusses protocols for the biochemical estimation of reactive oxygen species, metabolites, and stress enzymes
  • Provides various histological fixation methods for detecting the structural arrangement in the flight muscle, eye, gut, and hemolymph of Drosophila under various microscopes
  • Includes protocols for assessing the cytogenotoxicity, fat detection and metabolic activity in flies using fluorescent microscope and microplate reader
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eBook 139,09 €
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  • ISBN 978-1-4939-9756-5
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Hardcover 176,79 €
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  • ISBN 978-1-4939-9755-8
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About this book

This volume includes in-depth, hands-on protocols for detecting various developmental defects in Drosophila. It provides cutting-edge methods for maintaining Drosophila under laboratory conditions to perform various experiments, and for dissecting various imaginal discs of the larvae. Further, biochemical protocols for estimating the levels of different metabolites, reactive oxygen species, and fat-sensitive pathways are discussed, and various staining and behavior techniques for fat detection are provided.

The book explains how various fluorescent dyes and the comet assay can be used to identify DNA damage, and elaborates on the analysis of the eye, antennae, imaginal disc, gut and muscle damage under bright field and fluorescent microscopes. It covers the analysis of hemolymph using Giemsa staining; determining the functionality of channel proteins, eye and mechanosensory organs in adults and larvae using various behavioral assays; and metal detection and structural analysis for various tissues using SEM. In closing, it addresses the analysis of Drosophila’s eye and head via paraffin section; measurement of reactive oxygen species from various tissues via FACS; and the CRISPER technique for gene editing and analysis of micro RNA mutations.

About the authors

Dr. Monalisa Mishra is an Assistant Professor at the Department of Life Sciences, National Institute of Technology (NIT) in Rourkela, India. She completed her Ph.D. at the University of Bremen, Germany, prior to postdoctoral studies at the School of Medicine, University of San Diego, California, USA; Department of Biological Sciences, Indiana University, Bloomington, USA; and Max Plank Institute of Cell Biology and Genetics, Dresden, Germany.

Further, she previously served as an Assistant Professor at the Department of Biological Sciences, BITS in Pilani, India from 2012-2014. Currently, her lab at the NIT Rourkela is investigating various factors that can affect the development of Drosophila’s sensory organs. The lab’s findings have been published in many prominent international journals.


Table of contents (19 chapters)

Table of contents (19 chapters)
  • Formulation of Drosophila Food for Various Feeding Studies

    Pages 1-13

    Priyadarsini, Subhashree (et al.)

  • Isolation of Gut, Imaginal Disc, Fat Body, Ovary and Testes in Various Developmental Stages of Drosophila

    Pages 15-30

    Nayak, Nibedita (et al.)

  • Hemolymph Analysis of Drosophila melanogaster by Giemsa Staining

    Pages 31-38

    Bag, Janmejaya (et al.)

  • Histological Analysis of the Drosophila Head with Special Reference to the Eye and Brain

    Pages 39-49

    Karmakar, Puja (et al.)

  • Methodology to Detect the Abnormality of Drosophila Gut by Various Staining Techniques

    Pages 51-64

    Priyadarsini, Subhashree (et al.)

Buy this book

eBook 139,09 €
price for Spain (gross)
  • ISBN 978-1-4939-9756-5
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 176,79 €
price for Spain (gross)
  • ISBN 978-1-4939-9755-8
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
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Bibliographic Information

Bibliographic Information
Book Title
Fundamental Approaches to Screen Abnormalities in Drosophila
Editors
  • Monalisa Mishra
Series Title
Springer Protocols Handbooks
Copyright
2020
Publisher
Springer US
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9756-5
DOI
10.1007/978-1-4939-9756-5
Hardcover ISBN
978-1-4939-9755-8
Series ISSN
1949-2448
Edition Number
1
Number of Pages
VII, 265
Number of Illustrations
5 b/w illustrations, 111 illustrations in colour
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