Cryptosporidium
Methods and Protocols
Editors: Mead, Jan R., Arrowood, Michael J. (Eds.)
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- Provides step-by-step detail essential for reproducible results
- Contains key implementation advice from the experts
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- About this book
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This book encompasses broad aspects of Cryptosporidium research with established methods that have been improved and expanded over the years as well as recent cutting-edge techniques. Within this collection are numerous molecular methods as well as protocols for genotyping and diagnostics, while also including room for in vitro cell culture techniques to address the issues with growing this difficult organism continuously. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Cryptosporidium: Methods and Protocols serves as an idea guide to the inherent challenges of working with cryptosporidial parasites to provide a foundation for new investigators to build upon.
The chapter “Accessing Cryptosporidium Omic and Isolate Data via CryptoDB.org” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
- Table of contents (22 chapters)
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Cryptosporidium Diagnostic Assays: Microscopy
Pages 1-10
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Cryptosporidium Diagnostic Assays: Molecular Detection
Pages 11-22
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Detection of Cryptosporidium Recovered from Large-Volume Water Samples Using Dead-End Ultrafiltration
Pages 23-41
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Cryptosporidium Oocyst Purification Using Discontinuous Gradient Centrifugation
Pages 43-59
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Measuring Cryptosporidium Serologic Responses by Multiplex Bead Assay
Pages 61-85
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Table of contents (22 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Cryptosporidium
- Book Subtitle
- Methods and Protocols
- Editors
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- Jan R. Mead
- Michael J. Arrowood
- Series Title
- Methods in Molecular Biology
- Series Volume
- 2052
- Copyright
- 2020
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-4939-9748-0
- DOI
- 10.1007/978-1-4939-9748-0
- Hardcover ISBN
- 978-1-4939-9747-3
- Softcover ISBN
- 978-1-4939-9750-3
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XI, 407
- Number of Illustrations
- 22 b/w illustrations, 71 illustrations in colour
- Topics
