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Methods in Molecular Biology

In Vitro Differentiation of T-Cells

Methods and Protocols

Editors: Kaneko, Shin (Ed.)

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts
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eBook 129,99 €
price for Spain (gross)
  • ISBN 978-1-4939-9728-2
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  • Immediate eBook download after purchase
Hardcover 166,39 €
price for Spain (gross)
  • ISBN 978-1-4939-9727-5
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  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
About this book

This book explores the vital importance of T-cell differentiation in areas as wide-ranging as pathological analysis, drug development, and cell therapy of human T-cells. Focusing on human embryonic stem cells and human induced pluripotent stem cells, the chapters explore a variety of in vitro T-cell differentiation protocols as well as useful techniques to develop and evaluate cellular medicines. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, In Vitro Differentiation of T-Cells: Methods and Protocols serves as an ideal guide for researchers seeking to differentiate T-cells from pluripotent stem cells in order to achieve any number of significant goals.

Table of contents (20 chapters)

Table of contents (20 chapters)
  • Flow Cytometry Analysis to Identify Human CD8+ T Cells

    Pages 1-13

    Flynn, Jacqueline (et al.)

  • Flow Cytometry Analysis to Identify Human CD4+ T Cell Subsets

    Pages 15-25

    Flynn, Jacqueline (et al.)

  • Gene Modification and Immunological Analyses for the Development of Immunotherapy Utilizing T Cells Redirected with Antigen-Specific Receptors

    Pages 27-39

    Ochi, Toshiki (et al.)

  • In Vitro Conversion of Activated T Cells into Stem Cell Memory-Like T Cells

    Pages 41-51

    Kondo, Taisuke (et al.)

  • Human iPSC Generation from Antigen-Specific T Cells

    Pages 53-57

    Nishimura, Toshinobu (et al.)

Buy this book

eBook 129,99 €
price for Spain (gross)
  • ISBN 978-1-4939-9728-2
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 166,39 €
price for Spain (gross)
  • ISBN 978-1-4939-9727-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
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Bibliographic Information

Bibliographic Information
Book Title
In Vitro Differentiation of T-Cells
Book Subtitle
Methods and Protocols
Editors
  • Shin Kaneko
Series Title
Methods in Molecular Biology
Series Volume
2048
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9728-2
DOI
10.1007/978-1-4939-9728-2
Hardcover ISBN
978-1-4939-9727-5
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 267
Number of Illustrations
15 b/w illustrations, 45 illustrations in colour
Topics