- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Buy this book
- About this book
-
This volume presents the latest collection of immunophenotypic techniques and applications used in research and clinical settings. Chapters in this book cover topics such as constructions of high dimensions fluorescence and mass cytometry panels; fluorescence barcoding; using dried or lyophilized reagents; and immunophenotypic examples of specific cell types. The book concludes with a discussion on the critical roles of quality control and immunophenotyping in the clinical environment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Immunophenotyping: Methods and Protocols is a valuable resource for any researchers, clinician, or scientist interested in learning more about this evolving field.
- Table of contents (18 chapters)
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High-Dimensional Immunophenotyping with Fluorescence-Based Cytometry: A Practical Guidebook
Pages 1-29
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Immunophenotyping by Mass Cytometry
Pages 31-51
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Fluorescent Cell Barcoding for Immunophenotyping
Pages 53-68
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Immunophenotyping Using Dried and Lyophilized Reagents
Pages 69-79
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Guidelines for Gating Flow Cytometry Data for Immunological Assays
Pages 81-104
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Table of contents (18 chapters)
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Bibliographic Information
- Bibliographic Information
-
- Book Title
- Immunophenotyping
- Book Subtitle
- Methods and Protocols
- Editors
-
- J. Philip McCoy, Jr.
- Series Title
- Methods in Molecular Biology
- Series Volume
- 2032
- Copyright
- 2019
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-4939-9650-6
- DOI
- 10.1007/978-1-4939-9650-6
- Hardcover ISBN
- 978-1-4939-9649-0
- Softcover ISBN
- 978-1-4939-9652-0
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- X, 356
- Number of Illustrations
- 15 b/w illustrations, 79 illustrations in colour
- Topics
