Allergy

1. Front Matter

   Pages i-x

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2. Understanding of the Molecular Mechanisms of Allergy

   • Meinir G. Jones

   Pages 1-15

3. T Cell: Primary Culture from Peripheral Blood

   • Monika Raulf

   Pages 17-31

4. Measurement of Allergen-Specific Inhibitory Antibody Activity

   • Mohamed H. Shamji, James N. Francis

   Pages 33-43

5. Immunoelectrophoresis for the Characterization of Allergen Extracts

   • Gitte Nordskov Hansen, Jørgen Nedergaard Larsen

   Pages 45-62

6. Standardization of Allergen Extracts

   • Jørgen Nedergaard Larsen, Sten Dreborg

   Pages 63-76

7. Quantifying Human Innate Cytokine and Chemokine Responses Ex Vivo via Pattern Recognition Receptor Stimulation

   • Natascha Fitch, Stephanie J. Marshall, William P. Stefura, Rishma Chooniedass, Allan B. Becker, Kent T. HayGlass

   Pages 77-89

8. Improved Methods for Quantifying Human Chemokine and Cytokine Biomarker Responses: Ultrasensitive ELISA and Meso Scale Electrochemiluminescence Assays

   • William P. Stefura, Caroline Graham, Larisa Lotoski, Kent T. HayGlass

   Pages 91-114

9. Development of Mold Antigen-Specific Enzyme-Linked Immunosorbent Assays (ELISA) to Quantify Airborne Antigen Exposure

   • Eva Zahradnik, Monika Raulf

   Pages 115-130

10. Monocyte-Derived Dendritic Cells as Antigen-Presenting Cells in T-Cell Proliferation and Cytokine Production

    • Sun-Sang J. Sung

    Pages 131-141

11. The Use of Dual-Cell-Tracker Dye Staining for the Identification and Characterization of Peanut-Specific T-Cell Subsets

    • Cecilia Dunsterville, Alick C. Stephens, Gideon Lack, Victor Turcanu

    Pages 143-152

12. The Use of pMHCII Tetramer Technology for the Identification and Characterization of CD4+ T Cells

    • Erik Wambre

    Pages 153-164

13. Isolation and Characterization of Lymphocytes from Human Mucosal Biopsies

    • Nadine E. G. Upton, Thomas S. Hayday, Louisa K. James

    Pages 165-174

14. Development of a Dipstick Assay for Soy Allergens: Inexpensive Detection to Control Allergen Exposure

    • Daniel Álvarez-Simón, Susana Gómez-Ollés

    Pages 175-184

15. Novel Method for the Purification of House Dust Mite Allergen Der p 1 and Its Use in Structure-Based Chemical Design of Novel Inhibitors

    • Jihui Zhang, Clive Robinson

    Pages 185-205

16. Measurement of Specific IgG Anti-Fel d 1 Antibodies

    • Meinir G. Jones

    Pages 207-214

17. Measurement of Specific IgG4 Anti-mouse Urine Antibodies

    • Jennifer Canizales

    Pages 215-221

18. Preparation of Samples for a Mass Spectrometry-Based Method to Identify Allergenic Proteins

    • Mary Brian

    Pages 223-237

19. Back Matter

    Pages 239-240

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This second edition volume expands on the previous edition with new insights into the molecular mechanisms involved in allergy through the use of various protocols that cover disciplines like allergy, immunology, cell biology, and histology. The chapters in this book cover topics such as characterization of allergens using western blotting and mass spectrometry, collecting and sampling airborne allergens, IgG antibodies, identification and characterization of allergen specific T cells, and the role of antigen presenting cells in T cell proliferation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Cutting-edge and thorough, Allergy: Methods and Protocols, Second Edition is a valuable resource for researchers interested in beginning or continuing their research in the study of allergies. 

• antigen
• chemokine
• T cell
• cell biology
• cytokine
• immunology
• immunotherapy

“This well‐edited book with contributions by recognized experts is an important resource for bench researchers in this field. … The expertly written, detailed laboratory protocols with illustrations and specific notes should serve as a ready reference enabling investigators to achieve successful completion of the various determinations.” (Donald F. German, Doody's Book Reviews, November 08, 2019)

• Core Facilities, St. George’s University of London, London, UK

  Penny Lympany

• Department of Occupational and Environmental Medicine, Imperial College London, London, UK

  Meinir G. Jones

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