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Chimera Research

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Insoo Hyun

    1. Department of Bioethics, Case Western Reserve University, School of Medicine, Cleveland, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Alejandro De Los Angeles

    1. Department of Psychiatry, Yale University School of Medicine, New Haven, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2005)

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About this book

This volume addresses challenging new questions surrounding stem cell-based chimera research. This book is organized into three parts: Part One provides readers with a summary of different human donor cell types. The chapters in this section discuss ways to evaluate new types of pluripotent stem cells; the derivation of naïve and primed pluripotent stem cells from mouse preimplantation embryos; and the ethical and regulatory complexities of informed consent for the procurement of somatic cells. Part Two discusses methods for generating chimeras. The chapters here look at chick models and human-chick organizer grafts; generating human-pig interspecies chimeras; and techniques for transplanting mouse neural stem cells into a mouse disease model for stroke. Part Three concludes the book with a look at ongoing ethical controversies and new scientific directions. Chapters in this part cover the ethics of crossing the xenobarrier; animal welfare; experimentation with spermatogonial stem cells; and cautious approaches to human-monkey chimera studies to further understand complex human brain disorders. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Chimera Research: Methods and Protocols is a valuable resource for scientists interesting in using chimeras as a research tool while also taking into consideration their complex ethical scopes. 



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Table of contents (15 protocols)

  1. Front Matter

    Pages i-x
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  2. Human Donor Cell Types: Potency and Position

    1. Front Matter

      Pages 1-1
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    2. Frontiers of Pluripotency

      • Alejandro De Los Angeles
      Pages 3-27

    3. Highly Efficient Derivation of Pluripotent Stem Cells from Mouse Preimplantation and Postimplantation Embryos in Serum-Free Conditions

      • Alejandro De Los Angeles, Daiji Okamura, Jun Wu
      Pages 29-36

    4. Derivation of Hematopoietic Stem and Progenitor Cells from Human Pluripotent Stem Cells

      • Ryohichi Sugimura
      Pages 37-41

    5. Cancer Stem Cells: Concepts, Challenges, and Opportunities for Cancer Therapy

      • May Yin Lee, Rajshekhar R. Giraddi, Wai Leong Tam
      Pages 43-66

    6. Informed Consent Issues for Cell Donors

      • Insoo Hyun
      Pages 67-74

  3. Non-Human Hosts: Species and Developmental Stages

    1. Front Matter

      Pages 75-75
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    2. Chick Models and Human-Chick Organizer Grafts

      • Iain Martyn, Tatiane Y. Kanno, Ali H. Brivanlou
      Pages 77-89

    3. The Engraftment of Lentiviral Vector-Transduced Human CD34+ Cells into Humanized Mice

      • Yoon-Sang Kim, Matthew Wielgosz, Byoung Ryu
      Pages 91-100

    4. Pig Chimeric Model with Human Pluripotent Stem Cells

      • Cuiqing Zhong, Jun Wu, Juan Carlos Izpisua Belmonte
      Pages 101-124

    5. Embryonic Chimeras with Human Pluripotent Stem Cells

      • Alejandro De Los Angeles, Masahiro Sakurai, Jun Wu
      Pages 125-151

    6. Neural Stem Cell Transplantation into a Mouse Model of Stroke

      • Alejandro De Los Angeles
      Pages 153-163

    7. Ethical Standards for Chimera Research Oversight

      • Insoo Hyun
      Pages 165-171

  4. New Directions and Controversies

    1. Front Matter

      Pages 173-173
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    2. Ethical Considerations in Crossing the Xenobarrier

      • Sebastian Porsdam Mann, Rosa Sun, Göran Hermerén
      Pages 175-193

    3. Neurological Chimeras and the Moral Staircase

      • Daniel Counihan
      Pages 195-203

    4. Isolation, Cryopreservation, and Transplantation of Spermatogonial Stem Cells

      • Nilam Sinha, Eoin C. Whelan, Ralph L. Brinster
      Pages 205-220

    5. Human-Monkey Chimeras for Modeling Human Disease: Opportunities and Challenges

      • Alejandro De Los Angeles, Insoo Hyun, Stephen R. Latham, John D. Elsworth, D. Eugene Redmond Jr.
      Pages 221-231

  5. Back Matter

    Pages 233-234
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Editors and Affiliations

  • Department of Bioethics, Case Western Reserve University, School of Medicine, Cleveland, USA

    Insoo Hyun

  • Department of Psychiatry, Yale University School of Medicine, New Haven, USA

    Alejandro De Los Angeles

Bibliographic Information

  • Book Title: Chimera Research

  • Book Subtitle: Methods and Protocols

  • Editors: Insoo Hyun, Alejandro De Los Angeles

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9524-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9523-3Published: 08 June 2019

  • Softcover ISBN: 978-1-4939-9526-4Published: 14 August 2020

  • eBook ISBN: 978-1-4939-9524-0Published: 07 June 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 234

  • Number of Illustrations: 5 b/w illustrations, 23 illustrations in colour

  • Topics: Stem Cells, Human Genetics

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