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Microbial Lipid Production

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Venkatesh Balan

    1. Department of Engineering Technology, Biotechnology Program College of Technology, University of Houston, Houston, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1995)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Microbial Lipid Alternatives to Plant Lipids

    • A. Daniel Jones, Kyria L. Boundy-Mills, G. Florin Barla, Sandeep Kumar, Bryan Ubanwa, Venkatesh Balan
    Pages 1-32

  3. Laboratory Screening Protocol to Identify Novel Oleaginous Yeasts

    • Irnayuli R. Sitepu, Antonio L. Garay, Tomas Cajka , Oliver Fiehn, Kyria L. Boundy-Mills
    Pages 33-50

  4. Fungi (Mold)-Based Lipid Production

    • Yan Yang, Fatemeh Heidari, Bo Hu
    Pages 51-89

  5. Qualitative and Quantitative Estimation of Bacterial Lipid Production

    • Gangatharan Muralitharan, Manickam Gayathri, Sumathy Shunmugam
    Pages 91-101

  6. Rhodococcus and Yarrowia-Based Lipid Production Using Lignin-Containing Industrial Residues

    • Rosemary K. Le, Kristina M. Mahan, Arthur J. Ragauskas
    Pages 103-120

  7. Quantification of Lipid Content in Oleaginous Biomass Using Thermogravimetry

    • Balakrishna Maddi, Agasteswar Vadlamani, Sridhar Viamajala, Sasidhar Varanasi
    Pages 121-129

  8. Extraction and Characterization of Lipids from Macroalgae

    • David R. Nobles Jr., Schonna R. Manning
    Pages 131-140

  9. Genetic Engineering Approaches Used to Increase Lipid Production and Alter Lipid Profile in Microbes

    • Xiao-Ling Tang, Ya-Ping Xue
    Pages 141-150

  10. Extraction Methods Used to Separate Lipids from Microbes

    • Balakrishna Maddi
    Pages 151-159

  11. Novel Microbial Modification Tools to Convert Lipids into Other Value-Added Products

    • Priya Kumari, Farnaz Yusuf, Naseem A. Gaur
    Pages 161-171

  12. Alkaline and Alkaline-Oxidative Pretreatment and Hydrolysis of Herbaceous Biomass for Growth of Oleaginous Microbes

    • Jacob D. Crowe, Muyang Li, Daniel L. Williams, Alex D. Smith, Tongjun Liu, David B. Hodge
    Pages 173-182

  13. Laboratory Conversion of Cultivated Oleaginous Organisms into Biocrude for Biofuel Applications

    • Eboibi Blessing, Umakanta Jena, Senthil Chinnasamy
    Pages 183-193

  14. Life Cycle Analysis of Producing Microbial Lipids and Biodiesel: Comparison with Plant Lipids

    • Tom Bradley, Daniel Maga
    Pages 195-214

  15. Assessment of Fuel Quality Parameters and Selection of Bacteria Using PROMETHEE–GAIA Algorithm

    • Sumathy Shunmugam, Manickam Gayathri, Gangatharan Muralitharan
    Pages 215-227

  16. Production of High-Value Polyunsaturated Fatty Acids Using Microbial Cultures

    • Mingjie Jin, Rui Zhai, Zhaoxian Xu, Zhiqiang Wen
    Pages 229-248

  17. Screening for Oily Yeasts Able to Convert Hydrolysates from Biomass to Biofuels While Maintaining Industrial Process Relevance

    • Patricia J. Slininger, Bruce S. Dien, Joshua C. Quarterman, Stephanie R. Thompson, Cletus P. Kurtzman
    Pages 249-283

  18. Conversion of Microbial Lipids to Biodiesel and Basic Lab Tests for Analysis of Fuel-Quality Parameters

    • Annaliese K. Franz, Cody Yothers
    Pages 285-310

  19. Impact of Culture Conditions on Neutral Lipid Production by Oleaginous Yeast

    • Irene Fakankun, Maryam Mirzaei, David B. Levin
    Pages 311-325

  20. Producing Oleaginous Microorganisms Using Wastewater: Methods and Guidelines for Lab- and Industrial-Scale Production

    • Kayla M. Rude, Tyler J. Barzee, Annaliese K. Franz
    Pages 327-355
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Keywords

About this book

The volume explores methods for isolation and characterization of lipids, and their production using various oleaginous organisms. The protocols presented in the book consider sugars derived from different substrates including chemically pre-treated agricultural residues, industrial residues containing lignin, food wastes, and industrial waste water in an approachable format. The book also discusses applications that use oleaginous organisms to transform substrates into a variety of products including bio-crude, high value fatty acids, biofuels such as biodiesel, neutral lipids, volatile fatty acids, and surfactants. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



Comprehensive and cutting-edge, Microbial Lipid Production: Methods and Protocols is a valuable resource aimed at novice researchers who are working on improving their knowledge and skills in this developing field. 


Editors and Affiliations

  • Department of Engineering Technology, Biotechnology Program College of Technology, University of Houston, Houston, USA

    Venkatesh Balan

Bibliographic Information

  • Book Title: Microbial Lipid Production

  • Book Subtitle: Methods and Protocols

  • Editors: Venkatesh Balan

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9484-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9483-0Published: 31 May 2019

  • Softcover ISBN: 978-1-4939-9486-1Published: 14 August 2020

  • eBook ISBN: 978-1-4939-9484-7Published: 30 May 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 422

  • Number of Illustrations: 17 b/w illustrations, 41 illustrations in colour

  • Topics: Microbiology, Lipidology

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