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Cell-Based Assays Using iPSCs for Drug Development and Testing

  • Book
  • © 2019

Overview

Editors:
  1. Carl-Fredrik Mandenius

    1. Division of Biotechnology, Department of Physics, Chemistry and Biology (IFM), Linköping University, Linköping, Sweden

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    You can also search for this editor in PubMed   Google Scholar

  2. James A. Ross

    1. Tissue Injury and Repair Group, University of Edinburgh, Edinburgh, UK

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1994)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Cell-Based Assays Using Differentiated Human Induced Pluripotent Cells

    • Zameel Cader, Martin Graf, Mark Burcin, Carl-Fredrik Mandenius, James A. Ross
    Pages 1-14

  3. Differentiation of iPC to Target Cells for Assays

    1. Front Matter

      Pages 15-15
      Download chapter PDF

    2. Monolayer Generation of Vascular Endothelial Cells from Human Pluripotent Stem Cells

      • Klaus Christensen, Filip Roudnicky, Mark Burcin, Christoph Patsch
      Pages 17-29

    3. Generating Human iPSC-Derived Astrocytes with Chemically Defined Medium for In Vitro Disease Modeling

      • Katharina Janssen, Lamiaa Bahnassawy, Claudia Kiefer, Jürgen Korffmann, Georg C. Terstappen, Viktor Lakics et al.
      Pages 31-39

    4. Human Induced Pluripotent Stem Cell-Derived Definitive Endoderm Bulk Culture and Hepatic Differentiation

      • Anwar A. Palakkan, Jyoti Nanda, James A. Ross
      Pages 41-53

    5. Production of Cardiomyocytes from Human Pluripotent Stem Cells by Bioreactor Technologies

      • Caroline Halloin, Michelle Coffee, Felix Manstein, Robert Zweigerdt
      Pages 55-70

    6. Improved Protocol for Cardiac Differentiation and Maturation of Pluripotent Stem Cells

      • Imen Jebeniani, Shunli Ding, Michel Pucéat
      Pages 71-77

    7. Human Pluripotent Stem Cell Expansion in Stirred Tank Bioreactors

      • Felix Manstein, Caroline Halloin, Robert Zweigerdt
      Pages 79-91

    8. A Protocol for One-Step Differentiation of Human Induced Pluripotent Stem Cells into Mature Podocytes

      • Cormac Murphy, Elisabeth Feifel, Paul Jennings, Gerhard Gstraunthaler, Anja Wilmes
      Pages 93-99

    9. Differentiation of hiPS Cells into Definitive Endoderm for High-Throughput Screening

      • Teresa Bluhmki, Stefanie Traub, Eva Schruf, James Garnett, Florian Gantner, Daniel Bischoff et al.
      Pages 101-115

  4. High Throughput Metabolomics and Proteomic Cell-Based Assays

    1. Front Matter

      Pages 117-117
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    2. LC-MS/MS-Based Metabolomics for Cell Cultures

      • Anna Artati, Cornelia Prehn, Jerzy Adamski
      Pages 119-130

    3. Preparation of iPSCs for Targeted Proteomic Analysis

      • Anna Baud, Wendy E. Heywood, Daniel Little, Paul Gissen, Kevin Mills
      Pages 131-139

    4. Measurement of Bile Acids as a Marker of the Functionality of iPSC-Derived Hepatocytes

      • Francesca Mazzacuva, Maëlle Lorvellec, Agostino Cilibrizzi, Kevin Mills, Peter Clayton, Paul Gissen
      Pages 141-147

    5. Mass Spectrometry Measurement of Albumin–Alpha Fetoprotein Ratio as an Indicator of iPSC-Derived Hepatocyte Differentiation

      • Francesca Mazzacuva, Maëlle Lorvellec, Agostino Cilibrizzi, Kevin Mills, Wendy E. Heywood, Peter Clayton et al.
      Pages 149-156

  5. High Content Imaging

    1. Front Matter

      Pages 157-157
      Download chapter PDF

    2. Seeding Induced Pluripotent Stem Cell-Derived Neurons onto 384-Well Plates

      • Daniel Little, Christin Luft, Oliver Pezzini-Picart, Olukunbi Mosaku, Robin Ketteler, Michael J. Devine et al.
      Pages 159-164

    3. High-Content Autophagy Analysis in iPSC-Derived Neurons Using Immunofluorescence

      • Daniel Little, Christin Luft, Olukunbi Mosaku, Robin Ketteler, Michael J. Devine, Paul Gissen
      Pages 165-174

    4. High-Content Analysis of Mitochondrial Function in iPSC-Derived Neurons

      • Daniel Little, Christin Luft, Olukunbi Mosaku, Robin Ketteler, Michael J. Devine, Paul Gissen
      Pages 175-184
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Keywords

About this book

The volume presents a variety of protocols that will be useful to researchers working in the field of disease modeling, drug discovery, and the cell biology of various tissues. Chapters in this book cover topics such as requirements and preconditions for using human induced pluripotent cell lines in assay development; the culture of iPSCs and iPSC-derived cardiomyocytes by bioreactor technologies; distinctions between hepatocytes, vascular endothelial cells, cardiomyocytes, renal podocytes, and neurons; and high content assays of mitochondrial function and autophagy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 Authoritative and cutting-edge, Cell-Based Assay using iPSCs for Drug Development and Testing: Methods and Protocols is a valuable resource for researchers working in this important and developing field of study.


Editors and Affiliations

  • Division of Biotechnology, Department of Physics, Chemistry and Biology (IFM), Linköping University, Linköping, Sweden

    Carl-Fredrik Mandenius

  • Tissue Injury and Repair Group, University of Edinburgh, Edinburgh, UK

    James A. Ross

Bibliographic Information

  • Book Title: Cell-Based Assays Using iPSCs for Drug Development and Testing

  • Editors: Carl-Fredrik Mandenius, James A. Ross

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9477-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9476-2Published: 25 May 2019

  • Softcover ISBN: 978-1-4939-9479-3Published: 14 August 2020

  • eBook ISBN: 978-1-4939-9477-9Published: 23 May 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 266

  • Number of Illustrations: 14 b/w illustrations, 55 illustrations in colour

  • Topics: Pharmacology/Toxicology, Stem Cells

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