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Protein Acetylation

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Robert M. Brosh, Jr.

    1. NIH Biomedical Research Center, National Institute on Aging, National Institutes of Health, Baltimore, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key note and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1983)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xiii
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  2. Detection, Quantification, and Isolation of Acetylated Protein Targets

    1. Front Matter

      Pages 1-1
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    2. High-Resolution Mass Spectrometry to Identify and Quantify Acetylation Protein Targets

      • Birgit Schilling, Jesse G. Meyer, Lei Wei, Melanie Ott, Eric Verdin
      Pages 3-16

    3. Isolation of Proteins on Nascent Chromatin and Characterization by Quantitative Mass Spectrometry

      • Paula A. Agudelo Garcia, Miranda Gardner, Michael A. Freitas, Mark R. Parthun
      Pages 17-27

    4. Detection and Quantitation of Acetylated Histones on Replicating DNA Using In Situ Proximity Ligation Assay and Click-It Chemistry

      • Pavlo Lazarchuk, Sunetra Roy, Katharina Schlacher, Julia Sidorova
      Pages 29-45

  3. Lysine Acetylation

    1. Front Matter

      Pages 47-47
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    2. Quantification of In Vitro Protein Lysine Acetylation by Reversed Phase HPLC

      • Catherine W. Njeri, Onyekachi E. Ononye, Lata Balakrishnan
      Pages 49-56

    3. Measurement and Analysis of Lysine Acetylation by KAT Complexes In Vitro and In Vivo

      • Anahita Lashgari, Jean-Philippe Lambert, Jacques Côté
      Pages 57-77

    4. Site-Specific Lysine Acetylation Stoichiometry Across Subcellular Compartments

      • Anastasia J. Lindahl, Alexis J. Lawton, Josue Baeza, James A. Dowell, John M. Denu
      Pages 79-106

    5. Lysine Acetylation of Proteins and Its Characterization in Human Systems

      • David K. Orren, Amrita Machwe
      Pages 107-130

  4. Sirtuin Targets of Acetylation

    1. Front Matter

      Pages 131-131
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    2. Molecular and Cellular Characterization of SIRT1 Allosteric Activators

      • Michael B. Schultz, Conrad Rinaldi, Yuancheng Lu, João A. Amorim, David A. Sinclair
      Pages 133-149

    3. Assessment of SIRT2 Inhibitors in Mouse Models of Cancer

      • Yashira L. Negrón Abril, Irma Fernández, Robert S. Weiss
      Pages 151-171

  5. Modulation of DNA Repair by Acetylation

    1. Front Matter

      Pages 173-173
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    2. Using Yeast as a Model Organism to Study the Functional Roles of Histone Acetylation in DNA Excision Repair

      • Amelia J. Hodges, Steven A. Roberts, John J. Wyrick
      Pages 175-190

    3. Biochemical and Cellular Assays to Assess the Effects of Acetylation on Base Excision Repair Enzymes

      • Shrabasti Roychoudhury, Suravi Pramanik, Hannah L. Harris, Kishor K. Bhakat
      Pages 191-206

    4. Analysis of DNA Processing Enzyme FEN1 and Its Regulation by Protein Lysine Acetylation

      • Onyekachi E. Ononye, Catherine W. Njeri, Lata Balakrishnan
      Pages 207-224

    5. Examining the Role of HDACs in DNA Double-Strand Break Repair in Neurons

      • Ping-Chieh Pao, Jay Penney, Li-Huei Tsai
      Pages 225-234

  6. DNA Interactive Proteins and Processing Enzymes Affected by Acetylation

    1. Front Matter

      Pages 235-235
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    2. Experimental Approaches to Investigate the Role of Helicase Acetylation in Regulating R-Loop Stability

      • Chenlin Song, Ingrid Grummt
      Pages 237-253
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Keywords

About this book

The book focuses on the regulation of protein function and biological activity by the post-translational modification known as acetylation. Chapters detail a wide range of topics in protein acetylation dealing with pathways relevant to cellular homeostasis, gene expression, and DNA metabolism. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Protein Acetylation: Methods and Protocols aims to be an extremely valuable resource to novices and experts alike whose research takes them in the direction of characterizing regulatory switches in protein acetylation events, and how they are manifested in significant ways in diverse mechanisms biological systems.



Editors and Affiliations

  • NIH Biomedical Research Center, National Institute on Aging, National Institutes of Health, Baltimore, USA

    Robert M. Brosh, Jr.

Bibliographic Information

  • Book Title: Protein Acetylation

  • Book Subtitle: Methods and Protocols

  • Editors: Robert M. Brosh, Jr.

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9434-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9433-5Published: 14 May 2019

  • Softcover ISBN: 978-1-4939-9436-6Published: 14 August 2020

  • eBook ISBN: 978-1-4939-9434-2Published: 13 May 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 310

  • Number of Illustrations: 12 b/w illustrations, 40 illustrations in colour

  • Topics: Protein Science, Gene Expression, Posttranslational Modification

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