Methods in Molecular Biology

RNA Interference and Cancer Therapy

Methods and Protocols

Editors: Dinesh Kumar, Lekha (Ed.)

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts
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About this book

This comprehensive volume collects a repertoire of techniques for the adoption and exploitation of RNA interference (RNAi) as a fertile strategy to develop the bio-drugs of future in the vital field of cancer research. Exploring the immense ‘bench to bedside’ potential of RNAi as a therapeutic molecule, the book focuses many of its chapters on nanoparticle-based targeted deliveries, including aptamers, as well as techniques for using RNAi technology to combat various cancers in preclinical studies. Each of the 26 chapters is authored by the pioneers in the respective areas and thus provides a practical and empirical account of the techniques that could be used as benchmark tools in the area of cancer research. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and essential, RNA Interference and Cancer Therapy: Methods and Protocols provides the necessary guidance for both novice and professional researchers who intend to discover and innovate newer means of using RNAi technology to combat cancer, one of the greatest scourges of human health.

Table of contents (26 chapters)

Table of contents (26 chapters)
  • siRNA Library Screening to Identify Complementary Therapeutic Pairs in Triple-Negative Breast Cancer Cells

    Pages 1-19

    Thapa, Bindu (et al.)

  • In Vitro Evaluation of Candidate Gene Targets for Cancer Therapy

    Pages 21-30

    Tan, Xing Fei (et al.)

  • siRNA-Based Drug Targeting Human Bcl-xL Against Cancers

    Pages 31-40

    Takei, Yoshifumi

  • Relative Quantification of siRNA Strand Loading into Ago2 for Design of Highly Active siRNAs

    Pages 41-56

    Angart, Phillip A. (et al.)

  • VEGFA Gene Silencing in CXCR4-Expressing Cells via siRNA Delivery by Means of Targeted Peptide Carrier

    Pages 57-68

    Egorova, Anna A. (et al.)

Buy this book

eBook n/a
  • ISBN 978-1-4939-9220-1
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
Hardcover n/a
  • ISBN 978-1-4939-9219-5
  • Free shipping for individuals worldwide
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Bibliographic Information

Bibliographic Information
Book Title
RNA Interference and Cancer Therapy
Book Subtitle
Methods and Protocols
Editors
  • Lekha Dinesh Kumar
Series Title
Methods in Molecular Biology
Series Volume
1974
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9220-1
DOI
10.1007/978-1-4939-9220-1
Hardcover ISBN
978-1-4939-9219-5
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIII, 416
Number of Illustrations
28 b/w illustrations, 84 illustrations in colour
Topics