Non-Natural Nucleic Acids

1. Front Matter

   Pages i-xi

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2. Synthesis and Enzymatic Characterization of Sugar-Modified Nucleoside Triphosphate Analogs

   • Stella Diafa, Damien Evéquoz, Christian J. Leumann, Marcel Hollenstein

   Pages 1-13

3. Synthesis of Site-Specific Crown Ether Adducts to DNA Abasic Sites: 8-Oxo-7,8-Dihydro-2′-Deoxyguanosine and 2′-Deoxycytidine

   • Na An, Aaron M. Fleming, Nicole C. Rosecrans, Yi Liao, Cynthia J. Burrows

   Pages 15-25

4. Synthesis of a Fluorescent Cytidine TNA Triphosphate Analogue

   • Hui Mei, John Chaput

   Pages 27-37

5. Synthesis of Base-Modified dNTPs Through Cross-Coupling Reactions and Their Polymerase Incorporation to DNA

   • Petra Ménová, Hana Cahová, Milan Vrábel, Michal Hocek

   Pages 39-57

6. 2′-C,4′-C-Ethyleneoxy-Bridged 2′-Deoxyribonucleic Acids (EoDNAs) with Thymine Nucleobases: Synthesis, Duplex-Forming Ability, and Enzymatic Stability

   • Takashi Osawa, Satoshi Obika, Yoshiyuki Hari

   Pages 59-89

7. Synthesis Protocols for Simple Uncharged Glycol Carbamate Nucleic Acids

   • Tanaya Bose, Vaijayanti A. Kumar

   Pages 91-106

8. Synthesis of Nucleobase-Functionalized Morpholino Monomers

   • Bappaditya Nandi, Sankha Pattanayak, Sibasish Paul, Jayanta Kundu, Surajit Sinha

   Pages 107-130

9. Synthesis and Application of LKγT Peptide Nucleic Acids

   • Nathaniel Shank, Kara M. George Rosenker, Ethan A. Englund, Andrew V. Dix, Elizabeth E. Rastede, Daniel H. Appella

   Pages 131-145

10. Aminoglycoside Functionalization as a Tool for Targeting Nucleic Acids

    • Derrick Watkins, Krishnagopal Maiti, Dev P. Arya

    Pages 147-162

11. Preparation and Purification of Oligodeoxynucleotide Duplexes Containing a Site-Specific, Reduced, Chemically Stable Covalent Interstrand Cross-Link Between a Guanine Residue and an Abasic Site

    • Maryam Imani Nejad, Xu Guo, Kurt Housh, Christopher Nel, Zhiyu Yang, Nathan E. Price et al.

    Pages 163-175

12. Copper-Catalyzed Alkyne-Azide Cycloaddition on the Solid Phase for the Preparation of Fully Click-Modified Nucleic Acids

    • Malte Rosenthal, Franziska Pfeiffer, Günter Mayer

    Pages 177-183

13. Labeling Peptide Nucleic Acids with Indium-111

    • Igor G. Panyutin

    Pages 185-191

14. Site-Specific Labeling of DNA via PCR with an Expanded Genetic Alphabet

    • Michael P. Ledbetter, Denis A. Malyshev, Floyd E. Romesberg

    Pages 193-212

15. Flexible Nucleic Acids (FNAs) as Informational Molecules: Enzymatic Polymerization of fNTPs on DNA Templates and Nonenzymatic Oligomerization of RNA on FNA Templates

    • Maryline Chemama, Christopher Switzer

    Pages 213-236

16. Artificial Nucleosides as Diagnostic Probes to Measure Translesion DNA Synthesis

    • Jung-Suk Choi, Anthony Berdis

    Pages 237-249

17. FRET Assay for Ligands Targeting the Bacterial A-Site RNA

    • Renatus W. Sinkeldam, Yitzhak Tor

    Pages 251-260

18. The Use of Serinol Nucleic Acids as Ultrasensitive Molecular Beacons

    • Keiji Murayama, Hiromu Kashida, Hiroyuki Asanuma

    Pages 261-279

19. Oligonucleotide Primers with G8AE-Clamp Modifications for RT-qPCR Detection of the Low-Copy dsRNA

    • Timofei S. Zatsepin, Anna M. Varizhuk, Vladimir G. Dedkov, German A. Shipulin, Andrey V. Aralov

    Pages 281-297

20. Determining Steady-State Kinetics of DNA Polymerase Nucleotide Incorporation

    • Hailey L. Gahlon, Shana J. Sturla

    Pages 299-311

This volume provides relevant synthetic strategies, incorporation, and applications of non-natural nucleic acids. Chapters detail monomer synthesis, oligomer synthesis/construction, and applications allowing researchers to explore and determine which methodology or methodologies are relevant to their needs.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Non-Natural Nucleic Acids: Methods and Protocols aims to serve as a guide for researchers exploring their own inquiries and to provide a springboard for new endeavors.

• Non-natural DNA mimics
• Nanoscaffolds
• Bioorganic chemistry
• Site-specific labeling
• DNA

• Department of Chemistry and Biochemistry, Georgia Southern University, Savannah, USA

  Nathaniel Shank

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