Skip to main content
  • Book
  • © 2019

Microcalorimetry of Biological Molecules

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains keynotes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1964)

Buy it now

Buying options

eBook USD 149.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

This is a preview of subscription content, log in via an institution to check for access.

Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi
  2. Differential Scanning Calorimetry

    1. Front Matter

      Pages 1-1
    2. Value of DSC in Characterization and Optimization of Protein Stability

      • Katherine Bowers, Natalia Markova
      Pages 33-44
    3. Plasmatic Signature of Disease by Differential Scanning Calorimetry (DSC)

      • Philipp O. Tsvetkov, François Devred
      Pages 45-57
  3. Isothermal Titration Calorimetry

    1. Front Matter

      Pages 59-59
    2. Intrinsic Thermodynamics of Protein-Ligand Binding by Isothermal Titration Calorimetry as Aid to Drug Design

      • Vaida Paketurytė, Asta Zubrienė, John E. Ladbury, Daumantas Matulis
      Pages 61-74
    3. Isothermal Titration Calorimetry Measurements of Riboswitch-Ligand Interactions

      • Christopher P. Jones, Grzegorz Piszczek, Adrian R. Ferré-D’Amaré
      Pages 75-87
    4. ITC Studies of Ribosome/Antibiotics Interactions

      • Emma Schenckbecher, Benoît Meyer, Eric Ennifar
      Pages 89-98
    5. ITC Measurement for High-Affinity Aptamers Binding to Their Target Proteins

      • Ryo Amano, Tomohisa Furukawa, Taiichi Sakamoto
      Pages 119-128
    6. Thermodynamics of Molecular Machines Using Incremental ITC

      • Benoît Meyer, Cyrielle da Veiga, Philippe Dumas, Eric Ennifar
      Pages 129-140
    7. Characterization of Microtubule-Associated Proteins (MAPs) and Tubulin Interactions by Isothermal Titration Calorimetry (ITC)

      • Philipp O. Tsvetkov, Romain La Rocca, Soazig Malesinski, François Devred
      Pages 151-165
  4. Advanced Data Processing and Kinetics

    1. Front Matter

      Pages 167-167
    2. Tinkering with Binding Polynomials in Isothermal Titration Calorimetry

      • Rafael Claveria-Gimeno, Sonia Vega, Olga Abian, Adrian Velazquez-Campoy
      Pages 185-213
    3. Thermodynamic and Kinetic Analysis of Isothermal Titration Calorimetry Experiments by Using KinITC in AFFINImeter

      • Eva Muñoz, Juan Sabín, Javier Rial, Daniel Pérez, Eric Ennifar, Philippe Dumas et al.
      Pages 225-239

About this book

This volume provides methods on microcalorimetry approaches to investigate complex biological molecular systems. Chapters guide readers through Differential Scanning Calorimetry (DSC), Isothermal Titration Calorimetry (ITC), and advanced data processing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Microcalorimetry of Biological Molecules: Methods and Protocols aims to ensure successful results in the further study of this vital field.



Editors and Affiliations

  • Institut de Biologie Moléculaire et Cellulaire, Université de Strasbourg, CNRS, Strasbourg, France

    Eric Ennifar

Bibliographic Information

  • Book Title: Microcalorimetry of Biological Molecules

  • Book Subtitle: Methods and Protocols

  • Editors: Eric Ennifar

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9179-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9178-5Published: 01 April 2019

  • eBook ISBN: 978-1-4939-9179-2Published: 30 March 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 268

  • Number of Illustrations: 20 b/w illustrations, 40 illustrations in colour

  • Topics: Biochemistry, general

Buy it now

Buying options

eBook USD 149.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access