- Includes cutting-edge techniques
- Provides step-by-step detail essential for reproducible results
- Contains key implementation advice from the experts
Buy this book
- About this book
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This detailed volume guides readers through strategic planning and user-friendly guidelines in order to select the most suitable CRISPR-Cas system and target sites with high activity and specificity. Methods covering CRISPR gRNA design, CRISPR delivery, CRISPR activity quantification (indel quantification), and examples of applying CRISPR gene editing in human pluripotent stem cells, primary cells, gene therapy, and genetic screening are included. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and invaluable, CRISPR Gene Editing: Methods and Protocols will assist undergraduates, graduates, and researchers with detailed guidelines and methods for the vitally important CRISPR gene editing field.
Chapter 3 is available open access under a CC BY 4.0 license via link.springer.com.
- Table of contents (21 chapters)
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CRISPR-gRNA Design
Pages 3-11
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Tracking CRISPR’s Footprints
Pages 13-28
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Rapid Quantitative Evaluation of CRISPR Genome Editing by TIDE and TIDER
Pages 29-44
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Fast and Quantitative Identification of Ex Vivo Precise Genome Targeting-Induced Indel Events by IDAA
Pages 45-66
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Functional Evaluation of CRISPR Activity by the Dual-Fluorescent Surrogate System: C-Check
Pages 67-77
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Table of contents (21 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- CRISPR Gene Editing
- Book Subtitle
- Methods and Protocols
- Editors
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- Yonglun Luo
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1961
- Copyright
- 2019
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-4939-9170-9
- DOI
- 10.1007/978-1-4939-9170-9
- Hardcover ISBN
- 978-1-4939-9169-3
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XI, 362
- Number of Illustrations
- 15 b/w illustrations, 61 illustrations in colour
- Topics
