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Nanotechnology for Nucleic Acid Delivery

Methods and Protocols

  • Includes cutting-edge techniques
  • Provides step-by-step details essential for reproducible results
  • Contains key advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1943)

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-xv
  2. Synthesis of Bioreducible Polycations with Controlled Topologies

    • Ye-Zi You, Jun-Jie Yan, Fei Yu, Zhi-Qiang Yu, David Oupicky
    Pages 27-38
  3. Histidine-Rich Cationic Cell-Penetrating Peptides for Plasmid DNA and siRNA Delivery

    • Antoine Kichler, A. James Mason, Arnaud Marquette, Burkhard Bechinger
    Pages 39-59
  4. Synthesis of Polyethylenimine-Based Nanocarriers for Systemic Tumor Targeting of Nucleic Acids

    • Wolfgang Rödl, Alexander Taschauer, David Schaffert, Ernst Wagner, Manfred Ogris
    Pages 83-99
  5. Cationic Photopolymerized Polydiacetylenic (PDA) Micelles for siRNA Delivery

    • Manon Ripoll, Patrick Neuberg, Jean-Serge Remy, Antoine Kichler
    Pages 101-122
  6. Layer-by-Layer Assembled Nanoparticles for siRNA Delivery

    • Michaela Guter, Miriam Breunig
    Pages 153-160
  7. Layer-By-Layer Film Engineering for Sequential Gene Delivery

    • Lingxiao Xie, Yi Zou, Sean Carroll, Maria Muniz, Guangzhao Mao
    Pages 161-176
  8. Surface- and Hydrogel-Mediated Delivery of Nucleic Acid Nanoparticles

    • Angela K. Pannier, Tyler Kozisek, Tatiana Segura
    Pages 177-197
  9. In Situ AFM Analysis Investigating Disassembly of DNA Nanoparticles and Nanofilms

    • Yi Zou, Lei Wan, Jenifer Blacklock, Lingxiao Xie, Sean Carroll, David Oupicky et al.
    Pages 199-209
  10. Lyophilization of Synthetic Gene Carriers

    • Julia Christina Kasper, Sarah Hedtrich, Wolfgang Friess
    Pages 211-225
  11. Enhancing Nucleic Acid Delivery with Ultrasound and Microbubbles

    • Heleen Dewitte, Silke Roovers, Stefaan C. De Smedt, Ine Lentacker
    Pages 241-251
  12. Magnetic and Acoustically Active Microbubbles Loaded with Nucleic Acids for Gene Delivery

    • Dialechti Vlaskou, Olga Mykhaylyk, Christian Plank
    Pages 253-290
  13. Lactate Dehydrogenase Assay for Assessment of Polycation Cytotoxicity

    • Ladan Parhamifar, Helene Andersen, S. Moein Moghimi
    Pages 291-299

About this book

This detailed second edition volume expands upon the prior edition by addressing newly emerged technologies as well as improved existing protocols in nucleic acid delivery via nanotechnology. The book addresses topics ranging from chemical synthesis of macromolecules and bioconjugates, novel and established nanoformulations, characterization of these nanoformulations for biophysical, biological and toxicological aspects, and also protocols dealing with application and imaging of such carrier systems in vivo. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective chapters, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Nanotechnology for Nucleic Acid Delivery: Methods and Protocols, Second Edition serves as an ideal guide to researchers seeking to use this vital area of study in their own lab work or to progress knowledge within the field itself.

Editors and Affiliations

  • Laboratory of MacroMolecular Cancer Therapeutics (MMCT), Department of Pharmaceutical Chemistry, Center of Pharmaceutical Sciences, Faculty of Life Sciences, University of Vienna, Vienna, Austria

    Manfred Ogris, Haider Sami

Bibliographic Information

  • Book Title: Nanotechnology for Nucleic Acid Delivery

  • Book Subtitle: Methods and Protocols

  • Editors: Manfred Ogris, Haider Sami

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9092-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9091-7Published: 06 March 2019

  • eBook ISBN: 978-1-4939-9092-4Published: 05 March 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XV, 406

  • Number of Illustrations: 36 b/w illustrations, 71 illustrations in colour

  • Topics: Human Genetics, Nanotechnology, Biotechnology, Pharmacology/Toxicology

Buy it now

Buying options

eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access