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Kidney Organogenesis

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Seppo Vainio

    1. Biocenter Oulu, Laboratory of Developmental Biology, Oulu University, Oulu, Finland

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1926)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
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  2. Generation of Mosaic Embryonic Kidneys and Culture of the Kidney Tissues Ex Vivo

    1. Front Matter

      Pages 1-1
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    2. Generating Genetic Mosaic Mouse Embryos or Organoids for Studies of Kidney Development

      • Frank Costantini
      Pages 3-21

    3. Mouse Ex Vivo Kidney Culture Methods

      • Anneliis Ihermann-Hella, Satu Kuure
      Pages 23-30

    4. Serum-Free Organ Culture of the Embryonic Mouse Ureter

      • Filipa M. Lopes, Adrian S. Woolf
      Pages 31-38

    5. Experimental Tubulogenesis Induction Model in the Mouse

      • Aleksandra Rak-Raszewska
      Pages 39-51

  3. Embryonic Kidney Progenitor Cell and 3D Kidney Model Cell Like Based Cultures

    1. Front Matter

      Pages 53-53
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    2. Dissociation of Tissues for Single-Cell Analysis

      • Andrew S. Potter, S. Steven Potter
      Pages 55-62

    3. Long-Term Culture of Nephron Progenitor Cells Ex Vivo

      • Aaron C. Brown, Ashwani K. Gupta, Leif Oxburgh
      Pages 63-75

    4. 3D Cell Culture Models of Epithelial Tissues

      • Kai Zhang, Aki Manninen
      Pages 77-84

  4. Programming the Kidney Cell Fates in Human Pluripotent Cells

    1. Front Matter

      Pages 85-85
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    2. Generation of Three-Dimensional Nephrons from Mouse and Human Pluripotent Stem Cells

      • Yasuhiro Yoshimura, Atsuhiro Taguchi, Ryuichi Nishinakamura
      Pages 87-102

    3. Generating Multiple Kidney Progenitors and Cell Types from Human Pluripotent Stem Cells

      • Krithika Hariharan, Petra Reinke, Andreas Kurtz
      Pages 103-115

    4. Protocol to Generate Ureteric Bud Structures from Human iPS Cells

      • Shin-Ichi Mae, Makoto Ryosaka, Kenji Osafune
      Pages 117-123

  5. Protocols to Target the Functional Players in Kidney Ontogenesis

    1. Front Matter

      Pages 125-125
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    2. Investigating Aspects of Renal Physiology and Pharmacology in Organ and Organoid Culture

      • Melanie L. Lawrence, Mona Elhendawi, Jamie A. Davies
      Pages 127-142

    3. Virus as Renal Functional Genetic Tools

      • Abhishek Sharma, Sandhanakrishnan Cattavarayane
      Pages 143-149

    4. Gene Editing in 3D Cultured Nephron Progenitor Cell Lines

      • Zhongwei Li, Toshikazu Araoka, Juan Carlos Izpisua Belmonte
      Pages 151-159

  6. 4D and 3D Ex Vivo Imaging of Embryonic Kidney Decellularization

    1. Front Matter

      Pages 161-161
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    2. Time-Lapse Technologies and 4D Imaging of Kidney Development

      • Ulla Saarela, Ilya Skovorodkin
      Pages 163-167
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Keywords

About this book

This book details protocols on classical methods and very modern ex vivo mammalian developmental kidney model systems. Chapters focus on generation of mosaic 

embryonic kidneys, culture of the kidney tissues ex vivo, embryonic kidney progenitor cell, 3Dkidney model cell line based cultures, programming the kidney cell 
fates in human pluripotent cells, protocols to target the functional players in kidney ontogenesis, 4D and 3D ex vivo imaging, drosphila, and computational approaches to kidney ontogenesis. 
Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Kidney Organogenesis: Methods and Protocols aims to ensure successful results in thefurther study of this vital field.


Editors and Affiliations

  • Biocenter Oulu, Laboratory of Developmental Biology, Oulu University, Oulu, Finland

    Seppo Vainio

Bibliographic Information

  • Book Title: Kidney Organogenesis

  • Book Subtitle: Methods and Protocols

  • Editors: Seppo Vainio

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9021-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9020-7Published: 13 February 2019

  • eBook ISBN: 978-1-4939-9021-4Published: 11 February 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 248

  • Number of Illustrations: 13 b/w illustrations, 44 illustrations in colour

  • Topics: Molecular Medicine, Cell Culture, Nephrology

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