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Bone Research Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Aymen I. Idris

    1. Department of Oncology and Metabolism, University of Sheffield, Sheffield, UK

    View editor publications

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1914)

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Table of contents (35 protocols)

  1. Front Matter

    Pages i-xv
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  2. In Vitro Bone Cell Cultures

    1. Front Matter

      Pages 1-1
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    2. Isolation and Generation of Osteoclasts

      • Nadia Rucci, Alberta Zallone, Anna Teti
      Pages 3-19

    3. Isolation and Generation of Osteoblasts

      • Inês P. Perpétuo, Lucie E. Bourne, Isabel R. Orriss
      Pages 21-38

    4. Isolation, Purification, Generation, and Culture of Osteocytes

      • Jonathan H. Gooi, Ling Yeong Chia, Christina Vrahnas, Natalie A. Sims
      Pages 39-51

    5. Isolation, Differentiation, and Characterization of Human Bone Marrow Stem Cells In Vitro and In Vivo

      • Janos Kanczler, Rahul S. Tare, Patrick Stumpf, Timothy J. Noble, Cameron Black, Richard O. C. Oreffo
      Pages 53-70

    6. 2D and 3D In Vitro Co-Culture for Cancer and Bone Cell Interaction Studies

      • Silvia Marino, Ryan T. Bishop, Daniëlle de Ridder, Jesus Delgado-Calle, Michaela R. Reagan
      Pages 71-98

    7. Using Cell and Organ Culture Models to Analyze Responses of Bone Cells to Mechanical Stimulation

      • Soraia P. Caetano-Silva, Astrid Novicky, Behzad Javaheri, Simon C. F. Rawlinson, Andrew A. Pitsillides
      Pages 99-128

  3. Biochemical and Molecular Analysis of Bone Cells

    1. Front Matter

      Pages 129-129
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    2. Analysis of Signaling Pathways by Western Blotting and Immunoprecipitation

      • Silvia Marino, Aymen I. Idris
      Pages 131-143

    3. Analysis of Transcriptional Regulation in Bone Cells

      • Huilin Jin, Antonia Sophocleous, Asim Azfer, Stuart H. Ralston
      Pages 145-167

    4. Analysis of mRNA, miRNA, and DNA in Bone Cells by RT-qPCR and In Situ Hybridization

      • Brice Moukengue, Jérôme Amiaud, Camille Jacques, Céline Charrier, Benjamin Ory, Francois Lamoureux
      Pages 169-196

  4. Ex Vivo Models

    1. Front Matter

      Pages 197-197
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    2. The Ex Vivo Organ Culture of Bone

      • Katherine A. Staines, Genevieve Brown, Colin Farquharson
      Pages 199-215

    3. Ex-Vivo Model Systems of Cancer-Bone Cell Interactions

      • Erdjan Salih
      Pages 217-240

    4. Mechanical Properties of Bone Ex Vivo

      • Simon R. Goodyear, Richard M. Aspden
      Pages 241-257

  5. In Vivo Bone Models

    1. Front Matter

      Pages 259-259
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    2. Ovariectomy/Orchiectomy in Rodents

      • Antonia Sophocleous, Aymen I. Idris
      Pages 261-267

    3. In Vivo Models of Rheumatoid Arthritis

      • Bettina Grötsch, Aline Bozec, Georg Schett
      Pages 269-280

    4. Osteoarthritis Mouse Model of Destabilization of the Medial Meniscus

      • Antonia Sophocleous, Carmen Huesa
      Pages 281-293
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Keywords

About this book

The third edition of this book provides a large number of laboratory protocols that are intended for basic and clinical bone researchers. Chapters are organized into five parts covering methods for isolation, ex vivo models of bone organ and cell co-culture system, biochemical procedures, animal models of bone remodeling, and rodent models on Rheumatoid arthritis and osteoarthritis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge Bone Research Protocols: Methods and Protocols, Third Edition aims to extensive list of protocols for anyone interested in bone research.


Editors and Affiliations

  • Department of Oncology and Metabolism, University of Sheffield, Sheffield, UK

    Aymen I. Idris

Bibliographic Information

  • Book Title: Bone Research Protocols

  • Editors: Aymen I. Idris

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8997-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-8996-6Published: 08 February 2019

  • eBook ISBN: 978-1-4939-8997-3Published: 07 February 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XV, 670

  • Number of Illustrations: 51 b/w illustrations, 121 illustrations in colour

  • Topics: Molecular Medicine, Orthopedics

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