Methods in Molecular Biology

Electrophoretic Separation of Proteins

Methods and Protocols

Editors: Kurien, Biji T., Scofield, R. Hal (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook 178,49 €
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  • ISBN 978-1-4939-8793-1
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Hardcover 228,79 €
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About this book

This volume expands upon Protein Electrophoresis (2012) and provides readers with easy-to-follow and reproducible methods to study electrophoresis. The chapters in this book cover topics such as the Cydex Blue assay; cellulose-acetate electrophoresis of hemoglobin; cationic electrophoresis; tricine-SDS-Page; identification of proteins on archived 2-D gels; cell surface protein biotinylation of SDS-PAGE analysis; and artifacts and common errors in protein gel electrophoresis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and thorough, Electrophoretic Separation of Proteins: Methods and Protocols is a valuable resource for researchers who are interested in learning and experimenting with this field.

Table of contents (45 chapters)

Table of contents (45 chapters)
  • How It All Began: A Personal History of Gel Electrophoresis

    Smithies, Oliver

    Pages 1-21

  • Introduction to Protein Electrophoresis

    Srinivas, Pothur R.

    Pages 23-29

  • Measuring Protein Concentration with Absorbance, Lowry, Bradford Coomassie Blue, or the Smith Bicinchoninic Acid Assay Before Electrophoresis

    Goldring, J. P. Dean

    Pages 31-39

  • Concentrating Proteins by Salt, Polyethylene Glycol, Solvent, SDS Precipitation, Three-Phase Partitioning, Dialysis, Centrifugation, Ultrafiltration, Lyophilization, Affinity Chromatography, Immunoprecipitation or Increased Temperature for Protein Isolation, Drug Interaction, and Proteomic and Peptidomic Evaluation

    Goldring, J. P. Dean

    Pages 41-59

  • Lysis Buffer Choices Are Key Considerations to Ensure Effective Sample Solubilization for Protein Electrophoresis

    Miskiewicz, Ewa I. (et al.)

    Pages 61-72

Buy this book

eBook 178,49 €
price for Spain (gross)
  • ISBN 978-1-4939-8793-1
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 228,79 €
price for Spain (gross)
  • ISBN 978-1-4939-8792-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
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Bibliographic Information

Bibliographic Information
Book Title
Electrophoretic Separation of Proteins
Book Subtitle
Methods and Protocols
Editors
  • Biji T. Kurien
  • R. Hal Scofield
Series Title
Methods in Molecular Biology
Series Volume
1855
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-8793-1
DOI
10.1007/978-1-4939-8793-1
Hardcover ISBN
978-1-4939-8792-4
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XV, 523
Number of Illustrations
51 b/w illustrations, 61 illustrations in colour
Topics