Electrophoretic Separation of Proteins
Methods and Protocols
Editors: Kurien, Biji T., Scofield, R. Hal (Eds.)
Free Preview- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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- About this book
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This volume expands upon Protein Electrophoresis (2012) and provides readers with easy-to-follow and reproducible methods to study electrophoresis. The chapters in this book cover topics such as the Cydex Blue assay; cellulose-acetate electrophoresis of hemoglobin; cationic electrophoresis; tricine-SDS-Page; identification of proteins on archived 2-D gels; cell surface protein biotinylation of SDS-PAGE analysis; and artifacts and common errors in protein gel electrophoresis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and thorough, Electrophoretic Separation of Proteins: Methods and Protocols is a valuable resource for researchers who are interested in learning and experimenting with this field.
- Table of contents (45 chapters)
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How It All Began: A Personal History of Gel Electrophoresis
Pages 1-21
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Introduction to Protein Electrophoresis
Pages 23-29
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Measuring Protein Concentration with Absorbance, Lowry, Bradford Coomassie Blue, or the Smith Bicinchoninic Acid Assay Before Electrophoresis
Pages 31-39
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Concentrating Proteins by Salt, Polyethylene Glycol, Solvent, SDS Precipitation, Three-Phase Partitioning, Dialysis, Centrifugation, Ultrafiltration, Lyophilization, Affinity Chromatography, Immunoprecipitation or Increased Temperature for Protein Isolation, Drug Interaction, and Proteomic and Peptidomic Evaluation
Pages 41-59
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Lysis Buffer Choices Are Key Considerations to Ensure Effective Sample Solubilization for Protein Electrophoresis
Pages 61-72
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Table of contents (45 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Electrophoretic Separation of Proteins
- Book Subtitle
- Methods and Protocols
- Editors
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- Biji T. Kurien
- R. Hal Scofield
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1855
- Copyright
- 2019
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-4939-8793-1
- DOI
- 10.1007/978-1-4939-8793-1
- Hardcover ISBN
- 978-1-4939-8792-4
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XV, 523
- Number of Illustrations
- 51 b/w illustrations, 61 illustrations in colour
- Topics
